Restriction map for
pFN21AE3051
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# NO CUT ENZYMES ( 191 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BanI
BanII BbeI BbrPI BbvCI BbvI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoT22I FauI FbaI Fnu4HI FseI
FspI HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII HphI
ItaI KasI KpnI Ksp632I KspI
MaeII MamI MfeI MluI MroI
MslI MspA1I MspI MunI MvnI
MwoI NaeI NarI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TseI Tsp45I Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AciI 169 GCGG CCGC(-3/-1)
AcsI 152 GAATTC [GA]^AATT[CT]
ApoI 152 GAATTC [GA]^AATT[CT]
BalI 548 TGGCCA TGG^CCA
BamHI 262 GGATCC G^GATCC
BbsI 48 GTCTTC GAAGAC(2/6)
BfaI 250 CTAG C^TAG
BpmI 257 CTCCAG CTGGAG(16/14)
BpuAI 48 GTCTTC GAAGAC(2/6)
BsaJI 491 CCTTGG C^C[ACGT][ACGT]GG
BsmFI 209 GTCCC GGGAC(10/14)
BssKI 259 CCAGG ^CC[ACGT]GG
BstF5I 6 GGATG GGATG(2/0)
BstNI 259 CCAGG CC^[AT]GG
BstYI 262 GGATCC [GA]^GATC[CT]
CfoI 143 GCGC GCG^C
DdeI 512 CTCAG C^T[ACGT]AG
DraII 252 AGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 548 TGGCCA [CT]^GGCC[GA]
EcoO109I 252 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 152 GAATTC G^AATTC
EcoRII 259 CCAGG ^CC[AT]GG
EcoRV 295 GATATC GAT^ATC
EcoT14I 491 CCTTGG C^C[AT][AT]GG
FokI 6 GGATG GGATG(9/13)
HaeII 142 AGCGCC [GA]GCGC^[CT]
HhaI 143 GCGC GCG^C
HinP1I 143 GCGC G^CGC
MaeI 250 CTAG C^TAG
MaeIII 337 GTTAC ^GT[ACGT]AC
MflI 262 GGATCC [GA]^GATC[CT]
MluNI 548 TGGCCA TGG^CCA
MscI 548 TGGCCA TGG^CCA
MseI 371 TTAA T^TAA
MvaI 259 CCAGG CC^[AT]GG
PflMI 551 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 417 GAGTC GAGTC(4/5)
ScrFI 259 CCAGG CC^[ACGT]GG
SspI 367 AATATT AAT^ATT
StyI 491 CCTTGG C^C[AT][AT]GG
Tru9I 371 TTAA T^TAA
Van91I 551 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 262 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 14 enzymes) #
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AluI 31 AGCT AG^CT
164 AGCT
AlwI 263 GATCC GGATC(4/5)
262 GGATC
AvaII 208 GGTCC G^G[AT]CC
560 GGACC
Cac8I 22 GCCAGC GC[ACGT]^[ACGT]GC
165 GCTGGC
DpnII 239 GATC ^GATC
263 GATC
MboI 239 GATC ^GATC
263 GATC
NdeII 239 GATC ^GATC
263 GATC
NlaIII 111 CATG CATG^
538 CATG
NlaIV 208 GGTCCC GG[ACGT]^[ACGT]CC
262 GGATCC
Sau3AI 239 GATC ^GATC
263 GATC
TaqI 16 TCGA T^CGA
293 TCGA
TfiI 13 GATTC G^A[AT]TC
353 GAATC
TspRI 201 CAGTG CA[GC]TG[ACGT][ACGT]^
556 CAGTG
TthHB8I 16 TCGA T^CGA
293 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
#########################################
BseRI BsiYI BslI Cfr13I HaeIII
HinfI MboII MnlI Sau96I Tsp509I