Restriction map for
pFN21AE3753
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AluI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaHI BsaI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstXI BstZ17I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI Ksp632I KspI MboII
MfeI MluI MroI MslI MunI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SphI Sse8387I
SspBI StuI SwaI TaqI Tsp45I
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 176 GAATTC [GA]^AATT[CT]
AlwI 286 GGATC GGATC(4/5)
AlwNI 284 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 355 CCTAAGG CC^T[ACGT]AGG
ApoI 176 GAATTC [GA]^AATT[CT]
BalI 572 TGGCCA TGG^CCA
BpmI 438 CTGGAG CTGGAG(16/14)
BsaBI 58 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 566 ACCGGA [AT]^CCGG[AT]
BsmAI 242 GAGAC GTCTC(1/5)
BsmFI 276 GGGAC GGGAC(10/14)
BsrI 437 ACTGG ACTGG(1/-1)
BssKI 283 CCAGG ^CC[ACGT]GG
BstEII 360 GGTTACC G^GT[ACGT]ACC
BstF5I 569 GGATG GGATG(2/0)
BstNI 283 CCAGG CC^[AT]GG
BstPI 360 GGTTACC G^GT[ACGT]ACC
BstYI 286 GGATCT [GA]^GATC[CT]
Bsu36I 355 CCTAAGG CC^T[ACGT]AGG
DraII 276 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 572 TGGCCA [CT]^GGCC[GA]
Eco81I 355 CCTAAGG CC^T[ACGT]AGG
EcoO109I 276 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 360 GGTTACC G^GT[ACGT]ACC
EcoRI 176 GAATTC G^AATTC
EcoRII 283 CCAGG ^CC[AT]GG
EcoT14I 402 CCAAGG C^C[AT][AT]GG
FokI 569 GGATG GGATG(9/13)
HaeIII 573 GGCC GG^CC
HphI 331 TCACC GGTGA(8/7)
MaeII 69 ACGT A^CGT
MaeIII 361 GTTAC ^GT[ACGT]AC
MamI 58 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 286 GGATCT [GA]^GATC[CT]
MluNI 572 TGGCCA TGG^CCA
MscI 572 TGGCCA TGG^CCA
MspA1I 146 CCGCTG C[AC]G^C[GT]G
MvaI 283 CCAGG CC^[AT]GG
PflMI 575 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 441 GAGTC GAGTC(4/5)
PpuMI 276 GGGACCC [GA]G^G[AT]CC[CT]
ScrFI 283 CCAGG CC^[ACGT]GG
SfaNI 527 GCATC GCATC(5/9)
SfcI 222 CTGTAG C^T[GA][CT]AG
SspI 391 AATATT AAT^ATT
StyI 402 CCAAGG C^C[AT][AT]GG
TfiI 377 GAATC G^A[AT]TC
Van91I 575 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 286 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
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AciI 146 CCGC CCGC(-3/-1)
591 GCGG
AfaI 540 GTAC GT^AC
594 GTAC
Asp700I 73 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
100 GAACAAGTTC
BbvI 524 GCAGC GCAGC(8/12)
148 GCTGC
BfaI 41 CTAG C^TAG
164 CTAG
BsaJI 282 CCCAGG C^C[ACGT][ACGT]GG
402 CCAAGG
BseRI 212 CTCCTC GAGGAG(10/8)
469 CTCCTC
DdeI 8 CTGAG C^T[ACGT]AG
356 CTAAG
Fnu4HI 148 GCTGC GC^[ACGT]GC
524 GCAGC
HapII 445 CCGG C^CGG
567 CCGG
HincII 159 GTCAAC GT[CT]^[GA]AC
264 GTCAAC
HindII 159 GTCAAC GT[CT]^[GA]AC
264 GTCAAC
HinfI 377 GAATC G^A[ACGT]TC
441 GAGTC
HpaII 445 CCGG C^CGG
567 CCGG
ItaI 148 GCTGC GC^[ACGT]GC
524 GCAGC
MaeI 41 CTAG C^TAG
164 CTAG
MspI 445 CCGG C^CGG
567 CCGG
NlaIII 543 CATG CATG^
562 CATG
RsaI 540 GTAC GT^AC
594 GTAC
SpeI 40 ACTAGT A^CTAGT
163 ACTAGT
TseI 148 GCTGC G^C[AT]GC
524 GCAGC
Tsp509I 155 AATT ^AATT
177 AATT
XmnI 73 GAAAGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
100 GAACAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AvaII BsiYI BslI Cfr13I DpnII
MboI MnlI MseI NdeII NlaIV
Sau3AI Sau96I Tru9I TspRI