Restriction map for
pFN21AE1950
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AcyI AflII AgeI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspHI AspI AvaI AviII
AvrII BalI BanI BanII BbeI
BbrPI BbvCI BcgI BclI BcnI
BfrI BglI BglII BlnI BlpI
BmyI BpmI Bpu1102I BsaHI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsoBI Bsp1286I BspEI
BspLU11I BspMI BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I CpoI DraII DraIII DrdI
EaeI EagI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoT22I FauI
FbaI FseI FspI HaeII HapII
HgaI Hin1I HincII HindII HpaI
HpaII KasI KpnI KspI MfeI
MluI MluNI MroI MscI MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AclI 1411 AACGTT AA^CGTT
AcsI 453 GAATTC [GA]^AATT[CT]
AflIII 1377 ACACGT A^C[GA][CT]GT
AhdI 482 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 936 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 453 GAATTC [GA]^AATT[CT]
Asp700I 331 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 482 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 895 GGACC G^G[AT]CC
BamHI 1240 GGATCC G^GATCC
BciVI 1395 GGATAC GTATCC(6/5)
BsaBI 1048 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 203 GAGACC GGTCTC(1/5)
BsgI 1299 CTGCAC GTGCAG(16/14)
BsmI 1370 GAATGC GAATGC(1/-1)
Bsp1407I 66 TGTACA T^GTACA
BspDI 496 ATCGAT AT^CGAT
BspHI 48 TCATGA T^CATGA
BsrGI 66 TGTACA T^GTACA
BssKI 1133 CCTGG ^CC[ACGT]GG
BstNI 1133 CCTGG CC^[AT]GG
BstXI 1035 CCATATTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1240 GGATCC [GA]^GATC[CT]
CfoI 435 GCGC GCG^C
ClaI 496 ATCGAT AT^CGAT
DraI 415 TTTAAA TTT^AAA
Eam1105I 482 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1306 CCTACCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 453 GAATTC G^AATTC
EcoRII 1133 CCTGG ^CC[AT]GG
EcoRV 1165 GATATC GAT^ATC
HhaI 435 GCGC GCG^C
HinP1I 435 GCGC G^CGC
HindIII 1460 AAGCTT A^AGCTT
MamI 1048 GATAATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1240 GGATCC [GA]^GATC[CT]
MspA1I 152 CAGCTG C[AC]G^C[GT]G
MvaI 1133 CCTGG CC^[AT]GG
NcoI 373 CCATGG C^CATGG
NspI 688 ACATGC [GA]CATG^[CT]
Psp1406I 1411 AACGTT AA^CGTT
PvuII 152 CAGCTG CAG^CTG
RcaI 48 TCATGA T^CATGA
ScrFI 1133 CCTGG CC^[ACGT]GG
SnaBI 1448 TACGTA TAC^GTA
SspBI 66 TGTACA T^GTACA
XcmI 791 CCAACTTTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1240 GGATCC [GA]^GATC[CT]
XmnI 331 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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BbsI 352 GAAGAC GAAGAC(2/6)
1421 GTCTTC
BpuAI 352 GAAGAC GAAGAC(2/6)
1421 GTCTTC
BsaAI 1378 CACGTA [CT]AC^GT[GA]
1448 TACGTA
Cac8I 1139 GCTTGC GC[ACGT]^[ACGT]GC
1273 GCTTGC
Cfr13I 895 GGACC G^G[ACGT]CC
1010 GGGCC
DsaI 207 CCGTGG C^C[GA][CT]GG
373 CCATGG
EarI 859 GAAGAG CTCTTC(1/4)
1248 GAAGAG
EcoT14I 373 CCATGG C^C[AT][AT]GG
1355 CCAAGG
Ksp632I 859 GAAGAG CTCTTC(1/4)
1248 GAAGAG
MslI 228 CATTTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
823 CATGATAATG
PflMI 976 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1013 CCACTGAATGG
PleI 683 GAGTC GAGTC(4/5)
864 GAGTC
PstI 155 CTGCAG CTGCA^G
1430 CTGCAG
Sau96I 895 GGACC G^G[ACGT]CC
1010 GGGCC
SfcI 155 CTGCAG C^T[GA][CT]AG
1430 CTGCAG
StyI 373 CCATGG C^C[AT][AT]GG
1355 CCAAGG
TaqI 497 TCGA T^CGA
971 TCGA
TfiI 97 GAATC G^A[AT]TC
541 GATTC
TthHB8I 497 TCGA T^CGA
971 TCGA
Van91I 976 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1013 CCACTGAATGG
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# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AfaI AluI AlwI AseI AsnI
BbvI BfaI BsaJI BsiYI BslI
BsmAI BsrDI BsrI BstF5I DdeI
DpnII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MwoI
NdeII NlaIII NlaIV PshBI RsaI
Sau3AI SfaNI SmlI Tru9I TseI
Tsp45I Tsp509I TspRI