Restriction map for
pFN21AE3028
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvaII AviII AvrII
BalI BamHI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BlnI BlpI
BpmI Bpu1102I BsaAI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DdeI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoT14I FauI FbaI FseI FspI
HaeII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaI HpaII
KasI KpnI KspI MaeI MaeIII
MfeI MluI MluNI MroI MscI
MspA1I MspI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NspI NspV PacI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tsp45I Tsp509I XbaI
XcmI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 399 CGCG CG^CG
AcyI 241 GGCGTC G[GA]^CG[CT]C
AfaI 207 GTAC GT^AC
AlwI 339 GATCC GGATC(4/5)
AlwNI 137 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 13 GAGCAC G[AT]GC[AT]^C
AspI 103 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 25 CTCGAG C^[CT]CG[GA]G
BbsI 124 GAAGAC GAAGAC(2/6)
BglII 21 AGATCT A^GATCT
BpuAI 124 GAAGAC GAAGAC(2/6)
BsaBI 333 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 241 GGCGTC G[GA]^CG[CT]C
BsaI 80 GAGACC GGTCTC(1/5)
BsiHKAI 13 GAGCAC G[AT]GC[AT]^C
BsmFI 244 GTCCC GGGAC(10/14)
BsoBI 25 CTCGAG C^[CT]CG[GA]G
BstAPI 377 GCACCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 372 CATCC GGATG(2/0)
BstUI 399 CGCG CG^CG
BstXI 230 CCACGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 277 GCCTGC GC[ACGT]^[ACGT]GC
EcoNI 405 CCTGGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 46 GATATC GAT^ATC
EcoT22I 315 ATGCAT ATGCA^T
FokI 372 CATCC GGATG(9/13)
HgaI 242 GCGTC GACGC(5/10)
Hin1I 241 GGCGTC G[GA]^CG[CT]C
MaeII 128 ACGT A^CGT
MamI 333 GATCGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 426 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 399 CGCG CG^CG
NlaIII 314 CATG CATG^
NsiI 315 ATGCAT ATGCA^T
PaeR7I 25 CTCGAG C^TCGAG
PflFI 103 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 203 CCAAGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 417 CTGCAG CTGCA^G
RsaI 207 GTAC GT^AC
SapI 10 GAAGAGC GCTCTTC(1/4)
ScaI 206 AGTACT AGT^ACT
SexAI 68 ACCTGGT A^CC[AT]GGT
SmlI 25 CTCGAG C^T[CT][GA]AG
Tth111I 103 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 203 CCAAGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 25 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 17 enzymes) #
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BanI 148 GGCACC G^G[CT][GA]CC
309 GGTGCC
BmyI 13 GAGCAC G[AGT]GC[ACT]^C
147 GGGCAC
BsaJI 404 CCCTGG C^C[ACGT][ACGT]GG
405 CCTGGG
BsmAI 80 GAGAC GTCTC(1/5)
199 GTCTC
Bsp1286I 13 GAGCAC G[AGT]GC[ACT]^C
147 GGGCAC
BstYI 21 AGATCT [GA]^GATC[CT]
338 AGATCC
EarI 394 CTCTTC CTCTTC(1/4)
10 GAAGAG
HphI 72 GGTGA GGTGA(8/7)
177 GGTGA
Ksp632I 394 CTCTTC CTCTTC(1/4)
10 GAAGAG
MflI 21 AGATCT [GA]^GATC[CT]
338 AGATCC
MseI 95 TTAA T^TAA
320 TTAA
NlaIV 148 GGCACC GG[ACGT]^[ACGT]CC
309 GGTGCC
SfcI 153 CTACAG C^T[GA][CT]AG
417 CTGCAG
TaqI 26 TCGA T^CGA
335 TCGA
Tru9I 95 TTAA T^TAA
320 TTAA
TthHB8I 26 TCGA T^CGA
335 TCGA
XhoII 21 AGATCT [GA]^GATC[CT]
338 AGATCC
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI BbvI BsiYI BslI
BsrI BssKI BstNI Cfr13I DpnII
EcoRII Fnu4HI HaeIII ItaI MboI
MboII MnlI MvaI MwoI NdeII
Sau3AI Sau96I ScrFI SfaNI TseI
TspRI