Restriction map for
pFN21AE6254
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AgeI AhdI
Alw44I AlwI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaI BsaWI BseAI BseRI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstUI BstXI BstYI BstZ17I CelII
ClaI CpoI DpnII DraI DraIII
DrdI Eam1105I Eco47III EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HindIII HinfI HpaI KpnI KspI
MaeI MamI MboI MfeI MflI
MluI MluNI MroI MscI MunI
MvnI NcoI NdeI NdeII NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI Sau3AI ScaI SexAI
SfuI SgrAI SmlI SnaBI SpeI
SspBI SspI StuI SwaI TfiI
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XhoII
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AcyI 94 GGCGCC G[GA]^CG[CT]C
AflIII 14 ACGTGT A^C[GA][CT]GT
AocI 803 CCTCAGG CC^T[ACGT]AGG
Asp700I 744 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 428 TGCGCA TGC^GCA
BanI 94 GGCGCC G^G[CT][GA]CC
BbeI 94 GGCGCC GGCGC^C
BbsI 106 GTCTTC GAAGAC(2/6)
BpmI 649 CTGGAG CTGGAG(16/14)
BpuAI 106 GTCTTC GAAGAC(2/6)
BsaAI 655 TACGTG [CT]AC^GT[GA]
BsaHI 94 GGCGCC G[GA]^CG[CT]C
BsiEI 569 CGGCCG CG[GA][CT]^CG
BsmAI 282 GTCTC GTCTC(1/5)
BsrFI 792 GCCGGC [GA]^CCGG[CT]
Bsu36I 803 CCTCAGG CC^T[ACGT]AGG
Cfr10I 792 GCCGGC [GA]^CCGG[CT]
DsaI 444 CCACGG C^C[GA][CT]GG
EagI 569 CGGCCG C^GGCCG
EclXI 569 CGGCCG C^GGCCG
Eco52I 569 CGGCCG C^GGCCG
Eco81I 803 CCTCAGG CC^T[ACGT]AGG
FspI 428 TGCGCA TGC^GCA
HgaI 129 GACGC GACGC(5/10)
Hin1I 94 GGCGCC G[GA]^CG[CT]C
KasI 94 GGCGCC G^GCGCC
MseI 11 TTAA T^TAA
NaeI 792 GCCGGC GCC^GGC
NarI 94 GGCGCC GG^CGCC
NgoMIV 792 GCCGGC G^CCGGC
NspI 326 GCATGC [GA]CATG^[CT]
PshAI 129 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 255 CCTGCAGG CCTGCA^GG
SfaNI 348 GATGC GCATC(5/9)
SfoI 94 GGCGCC GGC^GCC
SmaI 113 CCCGGG CCC^GGG
SphI 326 GCATGC GCATG^C
Sse8387I 255 CCTGCAGG CCTGCA^GG
TaqI 137 TCGA T^CGA
Tru9I 11 TTAA T^TAA
TthHB8I 137 TCGA T^CGA
XmaCI 113 CCCGGG C^CCGGG
XmaI 113 CCCGGG C^CCGGG
XmnI 744 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AlwNI 280 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
316 CAGGGGCTG
AvaII 693 GGTCC G^G[AT]CC
833 GGTCC
BstAPI 279 GCAGTCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
384 GCACCTGCTGC
BstEII 216 GGTCACC G^GT[ACGT]ACC
549 GGTCACC
BstF5I 434 CATCC GGATG(2/0)
689 GGATG
BstPI 216 GGTCACC G^GT[ACGT]ACC
549 GGTCACC
DdeI 373 CTCAG C^T[ACGT]AG
804 CTCAG
EarI 190 CTCTTC CTCTTC(1/4)
199 CTCTTC
EcoNI 48 CCTCTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
679 CCTCTGACAGG
EcoO65I 216 GGTCACC G^GT[ACGT]ACC
549 GGTCACC
EcoT14I 74 CCAAGG C^C[AT][AT]GG
221 CCTTGG
FokI 434 CATCC GGATG(9/13)
689 GGATG
HaeII 94 GGCGCC [GA]GCGC^[CT]
450 GGCGCT
Ksp632I 190 CTCTTC CTCTTC(1/4)
199 CTCTTC
MslI 342 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
432 CACATCCGTG
MspA1I 525 CAGCGG C[AC]G^C[GT]G
533 CCGCTG
SacI 139 GAGCTC GAGCT^C
370 GAGCTC
SapI 189 GCTCTTC GCTCTTC(1/4)
198 GCTCTTC
StyI 74 CCAAGG C^C[AT][AT]GG
221 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AfaI AluI ApaI AspHI
AvaI BanII BbvI BcnI BglI
BmyI BsaJI BsgI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstNI Cac8I CfoI
Cfr13I DraII EaeI EcoO109I EcoRII
FauI Fnu4HI HaeIII HapII HhaI
HinP1I HpaII HphI ItaI MaeII
MaeIII MboII MnlI MspI MvaI
MwoI NciI NlaIII NlaIV PstI
RsaI Sau96I ScrFI SfcI SfiI
TseI Tsp45I TspRI