Restriction map for
pFN21AE3046
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccII AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI BspDI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HaeII HaeIII HhaI Hin1I HinP1I
HincII HindII HpaI HphI KasI
KpnI Ksp632I KspI MamI MboII
MluI MluNI MscI MslI MspA1I
MvnI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshBI PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I StuI
StyI SwaI TfiI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccIII 515 TCCGGA T^CCGGA
AciI 498 GCGG CCGC(-3/-1)
AclI 542 AACGTT AA^CGTT
AflIII 151 ACATGT A^C[GA][CT]GT
AvaII 47 GGTCC G^G[AT]CC
BanII 389 GGGCTC G[GA]GC[CT]^C
BcnI 50 CCCGG CC^[GC]GG
BfaI 10 CTAG C^TAG
BglII 308 AGATCT A^GATCT
BmyI 389 GGGCTC G[AGT]GC[ACT]^C
BsaI 37 GGTCTC GGTCTC(1/5)
BsaJI 228 CCAGGG C^C[ACGT][ACGT]GG
BsaWI 515 TCCGGA [AT]^CCGG[AT]
BseAI 515 TCCGGA T^CCGGA
Bsp1286I 389 GGGCTC G[AGT]GC[ACT]^C
Bsp1407I 148 TGTACA T^GTACA
BspEI 515 TCCGGA T^CCGGA
BspLU11I 151 ACATGT A^CATGT
BsrGI 148 TGTACA T^GTACA
BsrI 382 ACTGG ACTGG(1/-1)
BstNI 228 CCAGG CC^[AT]GG
BstYI 308 AGATCT [GA]^GATC[CT]
Cac8I 9 GCTAGC GC[ACGT]^[ACGT]GC
Cfr13I 47 GGTCC G^G[ACGT]CC
DdeI 349 CTGAG C^T[ACGT]AG
DraII 46 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 46 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRII 228 CCAGG ^CC[AT]GG
FauI 498 GCGGG CCCGC(4/6)
HgaI 67 GCGTC GACGC(5/10)
HindIII 181 AAGCTT A^AGCTT
MaeI 10 CTAG C^TAG
MaeII 543 ACGT A^CGT
MfeI 371 CAATTG C^AATTG
MflI 308 AGATCT [GA]^GATC[CT]
MroI 515 TCCGGA T^CCGGA
MunI 371 CAATTG C^AATTG
MvaI 228 CCAGG CC^[AT]GG
MwoI 58 GCAGCCACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 50 CCCGG CC^[GC]GG
NheI 9 GCTAGC G^CTAGC
NspI 151 ACATGT [GA]CATG^[CT]
PflMI 305 CCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 46 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 31 GACGATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 542 AACGTT AA^CGTT
PstI 482 CTGCAG CTGCA^G
Sau96I 47 GGTCC G^G[ACGT]CC
SfaNI 265 GATGC GCATC(5/9)
SfcI 482 CTGCAG C^T[GA][CT]AG
SspBI 148 TGTACA T^GTACA
SspI 472 AATATT AAT^ATT
TaqI 76 TCGA T^CGA
Tsp45I 29 GTGAC ^GT[GC]AC
TthHB8I 76 TCGA T^CGA
Van91I 305 CCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 308 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcsI 238 AAATTT [GA]^AATT[CT]
453 GAATTT
AfaI 149 GTAC GT^AC
270 GTAC
AlwI 172 GATCC GGATC(4/5)
463 GATCC
ApoI 238 AAATTT [GA]^AATT[CT]
453 GAATTT
BanI 279 GGTGCC G^G[CT][GA]CC
314 GGCACC
BbvI 58 GCAGC GCAGC(8/12)
481 GCTGC
BsmFI 500 GGGAC GGGAC(10/14)
48 GTCCC
BssKI 50 CCCGG ^CC[ACGT]GG
228 CCAGG
BstF5I 411 GGATG GGATG(2/0)
528 GGATG
Fnu4HI 58 GCAGC GC^[ACGT]GC
481 GCTGC
FokI 411 GGATG GGATG(9/13)
528 GGATG
HapII 51 CCGG C^CGG
516 CCGG
HinfI 73 GACTC G^A[ACGT]TC
78 GACTC
HpaII 51 CCGG C^CGG
516 CCGG
ItaI 58 GCAGC GC^[ACGT]GC
481 GCTGC
MspI 51 CCGG C^CGG
516 CCGG
NlaIII 152 CATG CATG^
276 CATG
PleI 73 GACTC GAGTC(4/5)
78 GACTC
RsaI 149 GTAC GT^AC
270 GTAC
ScrFI 50 CCCGG CC^[ACGT]GG
228 CCAGG
TseI 58 GCAGC G^C[AT]GC
481 GCTGC
TspRI 381 CACTG CA[GC]TG[ACGT][ACGT]^
467 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
AluI BsiYI BslI BsmAI DpnII
MaeIII MboI MnlI MseI NdeII
NlaIV Sau3AI Tru9I Tsp509I