##################################
# NO CUT ENZYMES (  195 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AcsI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BsgI        BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     Bsu36I      
CelII       CfoI        Cfr10I      ClaI        CpoI        
DraI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
HgaI        HhaI        HinP1I      HincII      HindII      
HindIII     HinfI       HpaI        HphI        KasI        
KpnI        Ksp632I     KspI        MaeI        MaeIII      
MamI        MboII       MfeI        MluI        MluNI       
MroI        MscI        MseI        MslI        MspA1I      
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
TaqI        TfiI        Tru9I       Tsp45I      Tsp509I     
TspRI       Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   37 enzymes) #
###################################

AatII         184  GACGTC               GACGT^C
AccII         133  CGCG                 CG^CG
AcyI          184  GACGTC               G[GA]^CG[CT]C
AluI           60  AGCT                 AG^CT
BbvCI           9  GCTGAGG              CCTCAGC(-5/-2)
BbvI           61  GCTGC                GCAGC(8/12)
BcnI          209  CCCGG                CC^[GC]GG
BsaHI         184  GACGTC               G[GA]^CG[CT]C
BseRI         165  GAGGAG               GAGGAG(10/8)
BsiEI         159  CGGTCG               CG[GA][CT]^CG
BspMI         217  ACCTGC               ACCTGC(4/8)
BsrI          128  CCAGT                ACTGG(1/-1)
BstF5I        255  GGATG                GGATG(2/0)
BstUI         133  CGCG                 CG^CG
BstYI          92  AGATCC               [GA]^GATC[CT]
DdeI           10  CTGAG                C^T[ACGT]AG
DraII          13  AGGGCCT              [GA]G^G[ACGT]CC[CT]
DsaI           86  CCATGG               C^C[GA][CT]GG
EcoO109I       13  AGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I        86  CCATGG               C^C[AT][AT]GG
FokI          255  GGATG                GGATG(9/13)
HapII         210  CCGG                 C^CGG
Hin1I         184  GACGTC               G[GA]^CG[CT]C
HpaII         210  CCGG                 C^CGG
MaeII         185  ACGT                 A^CGT
MflI           92  AGATCC               [GA]^GATC[CT]
MspI          210  CCGG                 C^CGG
MvnI          133  CGCG                 CG^CG
NciI          209  CCCGG                CC^[GC]GG
NcoI           86  CCATGG               C^CATGG
NlaIII         87  CATG                 CATG^
NlaIV          20  GGCTCC               GG[ACGT]^[ACGT]CC
SfaNI          36  GATGC                GCATC(5/9)
StuI          142  AGGCCT               AGG^CCT
StyI           86  CCATGG               C^C[AT][AT]GG
TseI           61  GCTGC                G^C[AT]GC
XhoII          92  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AfaI           71  GTAC                 GT^AC
              123  GTAC                
AlwI           93  GATCC                GGATC(4/5)
              137  GGATC               
Cac8I          29  GCCTGC               GC[ACGT]^[ACGT]GC
              200  GCCTGC              
DpnII          93  GATC                 ^GATC
              138  GATC                
FauI           54  GCGGG                CCCGC(4/6)
              134  GCGGG               
Fnu4HI         61  GCTGC                GC^[ACGT]GC
              152  GCCGC               
ItaI           61  GCTGC                GC^[ACGT]GC
              152  GCCGC               
MboI           93  GATC                 ^GATC
              138  GATC                
NdeII          93  GATC                 ^GATC
              138  GATC                
RsaI           71  GTAC                 GT^AC
              123  GTAC                
Sau3AI         93  GATC                 ^GATC
              138  GATC                


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

AciI        BsaJI       BsiYI       BslI        BsmFI       
BssKI       BstNI       Cfr13I      EcoRII      HaeIII      
MnlI        MvaI        MwoI        Sau96I      ScrFI