Restriction map for
pFN21AE9281
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# NO CUT ENZYMES ( 167 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AfaI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstPI Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT14I EcoT22I FauI FbaI FseI
FspI HapII Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MaeII MaeIII MamI MfeI
MluI MluNI MroI MscI MspA1I
MspI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI Tsp45I Tth111I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 372 GTATAC GT^[AC][GT]AC
AccII 346 CGCG CG^CG
AlwNI 548 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 509 GGGCCC GGGCC^C
AspHI 70 GAGCTC G[AT]GC[AT]^C
AvaI 73 CTCGAG C^[CT]CG[GA]G
AvaII 303 GGTCC G^G[AT]CC
BbvI 400 GCAGC GCAGC(8/12)
BcgI 390 CGATGATTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 121 CTAG C^TAG
BsaI 360 GGTCTC GGTCTC(1/5)
BsiHKAI 70 GAGCTC G[AT]GC[AT]^C
BsoBI 73 CTCGAG C^[CT]CG[GA]G
BspMI 183 ACCTGC ACCTGC(4/8)
Bst1107I 372 GTATAC GTA^TAC
BstUI 346 CGCG CG^CG
BstXI 473 CCACAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 263 GGATCT [GA]^GATC[CT]
BstZ17I 372 GTATAC GTA^TAC
EarI 424 GAAGAG CTCTTC(1/4)
EcoRV 161 GATATC GAT^ATC
Fnu4HI 400 GCAGC GC^[ACGT]GC
HaeII 25 AGCGCC [GA]GCGC^[CT]
HgaI 344 GACGC GACGC(5/10)
ItaI 400 GCAGC GC^[ACGT]GC
Ksp632I 424 GAAGAG CTCTTC(1/4)
MaeI 121 CTAG C^TAG
MboII 424 GAAGA GAAGA(8/7)
MflI 263 GGATCT [GA]^GATC[CT]
MseI 527 TTAA T^TAA
MslI 474 CACAAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 346 CGCG CG^CG
NlaIII 147 CATG CATG^
PaeR7I 73 CTCGAG C^TCGAG
PflMI 103 CCAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 70 GAGCTC GAGCT^C
SfaNI 331 GATGC GCATC(5/9)
SfcI 419 CTGTAG C^T[GA][CT]AG
TaqI 74 TCGA T^CGA
Tru9I 527 TTAA T^TAA
TseI 400 GCAGC G^C[AT]GC
TthHB8I 74 TCGA T^CGA
Van91I 103 CCAGTGTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 470 CCACCACAAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 73 CTCGAG C^TCGAG
XhoII 263 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 27 enzymes) #
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AciI 10 GCGG CCGC(-3/-1)
19 GCGG
AcsI 201 AAATTC [GA]^AATT[CT]
218 GAATTT
AlwI 51 GGATC GGATC(4/5)
263 GGATC
ApoI 201 AAATTC [GA]^AATT[CT]
218 GAATTT
BanII 70 GAGCTC G[GA]GC[CT]^C
509 GGGCCC
BmyI 70 GAGCTC G[AGT]GC[ACT]^C
509 GGGCCC
BpmI 557 CTCCAG CTGGAG(16/14)
83 CTGGAG
BsiYI 103 CCAGTGTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
499 CCAGTTGCAGG
BslI 103 CCAGTGTATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
499 CCAGTTGCAGG
BsmBI 298 GAGACG CGTCTC(1/5)
342 GAGACG
Bsp1286I 70 GAGCTC G[AGT]GC[ACT]^C
509 GGGCCC
BstF5I 454 GGATG GGATG(2/0)
516 GGATG
Cac8I 459 GCAAGC GC[ACGT]^[ACGT]GC
552 GCCTGC
CfoI 26 GCGC GCG^C
347 GCGC
DdeI 296 CTGAG C^T[ACGT]AG
325 CTTAG
DpnII 52 GATC ^GATC
264 GATC
FokI 454 GGATG GGATG(9/13)
516 GGATG
HaeIII 78 GGCC GG^CC
510 GGCC
HhaI 26 GCGC GCG^C
347 GCGC
HinP1I 26 GCGC G^CGC
347 GCGC
HinfI 239 GAATC G^A[ACGT]TC
466 GATTC
HphI 12 GGTGA GGTGA(8/7)
285 TCACC
MboI 52 GATC ^GATC
264 GATC
NdeII 52 GATC ^GATC
264 GATC
Sau3AI 52 GATC ^GATC
264 GATC
SmlI 73 CTCGAG C^T[CT][GA]AG
365 CTCAAG
TfiI 239 GAATC G^A[AT]TC
466 GATTC
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AluI BsaJI BsmAI BsrI BssKI
BstNI Cfr13I DraII EcoO109I EcoRII
MnlI MvaI MwoI NlaIV Sau96I
ScrFI Tsp509I TspRI