Restriction map for
pFN21AB8519
##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
Acc65I AccII AclI AgeI Alw44I
AlwNI Aor51HI ApaI ApaLI AscI
Asp718I AspHI AspI AviII BbeI
BbrPI BbvCI BglII BlpI Bpu1102I
BsaBI BsiHKAI BsiWI BsmBI Bsp1407I
BspDI BsrBI BsrFI BsrGI BssHII
BssSI BstEII BstPI BstUI BstXI
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI Eco47III EcoO65I FauI
FseI FspI HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MluI MvnI NaeI NarI NgoMIV
NotI NruI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AatII 481 GACGTC GACGT^C
AccIII 161 TCCGGA T^CCGGA
AciI 14 CCGC CCGC(-3/-1)
AflIII 1809 ACATGT A^C[GA][CT]GT
AhdI 476 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 2005 ATTAAT AT^TAAT
AsnI 2005 ATTAAT AT^TAAT
AspEI 476 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2645 CCCGAG C^[CT]CG[GA]G
AvrII 1545 CCTAGG C^CTAGG
BalI 2174 TGGCCA TGG^CCA
BamHI 838 GGATCC G^GATCC
BanI 814 GGCACC G^G[CT][GA]CC
BanII 1563 GGGCTC G[GA]GC[CT]^C
BcgI 1406 GCAATGATATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 862 CCGGG CC^[GC]GG
BglI 181 GCCTTAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1545 CCTAGG C^CTAGG
BsaAI 2044 TACGTG [CT]AC^GT[GA]
BsaWI 161 TCCGGA [AT]^CCGG[AT]
BseAI 161 TCCGGA T^CCGGA
BsgI 1463 CTGCAC GTGCAG(16/14)
BsiEI 11 CGGCCG CG[GA][CT]^CG
BsmI 81 GAATGC GAATGC(1/-1)
BsoBI 2645 CCCGAG C^[CT]CG[GA]G
BspEI 161 TCCGGA T^CCGGA
BspLU11I 1809 ACATGT A^CATGT
BspMI 2215 GCAGGT ACCTGC(4/8)
Bst1107I 1749 GTATAC GTA^TAC
BstAPI 2434 GCATATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 838 GGATCC [GA]^GATC[CT]
BstZ17I 1749 GTATAC GTA^TAC
EagI 11 CGGCCG C^GGCCG
Eam1105I 476 GACTGGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 11 CGGCCG C^GGCCG
Eco52I 11 CGGCCG C^GGCCG
EcoRI 1083 GAATTC G^AATTC
EcoT22I 309 ATGCAT ATGCA^T
HaeII 2185 GGCGCT [GA]GCGC^[CT]
MfeI 374 CAATTG C^AATTG
MflI 838 GGATCC [GA]^GATC[CT]
MluNI 2174 TGGCCA TGG^CCA
MroI 161 TCCGGA T^CCGGA
MscI 2174 TGGCCA TGG^CCA
MunI 374 CAATTG C^AATTG
NciI 862 CCGGG CC^[GC]GG
NdeI 2266 CATATG CA^TATG
NheI 766 GCTAGC G^CTAGC
NsiI 309 ATGCAT ATGCA^T
PshBI 2005 ATTAAT AT^TAAT
PstI 1015 CTGCAG CTGCA^G
ScaI 588 AGTACT AGT^ACT
XbaI 423 TCTAGA T^CTAGA
XcmI 326 CCAAACAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 838 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 124 GTAGAC GT^[AC][GT]AC
1749 GTATAC
AocI 676 CCTCAGG CC^T[ACGT]AGG
952 CCTAAGG
Asp700I 20 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2118 GAATCAGTTC
BciVI 1287 GGATAC GTATCC(6/5)
2745 GGATAC
BsaI 2686 GAGACC GGTCTC(1/5)
1324 GGTCTC
BstBI 803 TTCGAA TT^CGAA
1733 TTCGAA
Bsu36I 676 CCTCAGG CC^T[ACGT]AGG
952 CCTAAGG
CfoI 1944 GCGC GCG^C
2186 GCGC
DsaI 1454 CCATGG C^C[GA][CT]GG
1760 CCATGG
EaeI 11 CGGCCG [CT]^GGCC[GA]
2174 TGGCCA
Eco81I 676 CCTCAGG CC^T[ACGT]AGG
952 CCTAAGG
EcoRV 691 GATATC GAT^ATC
1411 GATATC
HgaI 1799 GACGC GACGC(5/10)
2285 GCGTC
HhaI 1944 GCGC GCG^C
2186 GCGC
HinP1I 1944 GCGC G^CGC
2186 GCGC
MslI 1357 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2161 CATGATGATG
NcoI 1454 CCATGG C^CATGG
1760 CCATGG
NspI 551 GCATGT [GA]CATG^[CT]
1809 ACATGT
NspV 803 TTCGAA TT^CGAA
1733 TTCGAA
PvuII 1532 CAGCTG CAG^CTG
2131 CAGCTG
SfuI 803 TTCGAA TT^CGAA
1733 TTCGAA
XmnI 20 GAATTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2118 GAATCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AcsI AcyI AfaI AflII AluI
AlwI ApoI AvaII BbsI BbvI
BclI BfaI BfrI BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BspHI
BsrDI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoNI EcoO109I EcoRII EcoT14I
FbaI Fnu4HI FokI HaeIII HapII
Hin1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PflMI PleI PpuMI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I