Restriction map for
pFN21AE1883
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# NO CUT ENZYMES ( 138 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BalI BbeI BbrPI BbsI BbvCI
BciVI BclI BcnI BfrI BglI
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaI BseRI BsiEI
BsiWI Bsp1407I BspDI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EaeI EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV FauI FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HpaI
KasI KpnI KspI MaeII MfeI
MluI MluNI MscI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NspV PacI
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacII SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I XbaI XcmI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccIII 449 TCCGGA T^CCGGA
AcsI 1002 AAATTT [GA]^AATT[CT]
AfaI 750 GTAC GT^AC
AflIII 980 ACATGT A^C[GA][CT]GT
AhdI 7 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 1002 AAATTT [GA]^AATT[CT]
AspEI 7 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 888 GGTGCC G^G[CT][GA]CC
BanII 816 GAGCTC G[GA]GC[CT]^C
BcgI 1123 GCAAGATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 124 CTAG C^TAG
BglII 549 AGATCT A^GATCT
BsaBI 876 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 449 TCCGGA T^CCGGA
BsgI 901 GTGCAG GTGCAG(16/14)
BsmBI 333 CGTCTC CGTCTC(1/5)
BsmFI 543 GTCCC GGGAC(10/14)
BsmI 387 GAATGC GAATGC(1/-1)
BspEI 449 TCCGGA T^CCGGA
BspHI 177 TCATGA T^CATGA
BspLU11I 980 ACATGT A^CATGT
BsrDI 1119 CATTGC GCAATG(2/0)
DdeI 812 CTGAG C^T[ACGT]AG
Eam1105I 7 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 55 CTCTTC CTCTTC(1/4)
EcoT22I 497 ATGCAT ATGCA^T
HincII 723 GTCGAC GT[CT]^[GA]AC
HindII 723 GTCGAC GT[CT]^[GA]AC
HindIII 1091 AAGCTT A^AGCTT
Ksp632I 55 CTCTTC CTCTTC(1/4)
MaeI 124 CTAG C^TAG
MamI 876 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 449 TCCGGA T^CCGGA
NsiI 497 ATGCAT ATGCA^T
PaeR7I 115 CTCGAG C^TCGAG
PflMI 35 CCATGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 9 CTGCAG CTGCA^G
RcaI 177 TCATGA T^CATGA
RsaI 750 GTAC GT^AC
SacI 816 GAGCTC GAGCT^C
SalI 723 GTCGAC G^TCGAC
SmlI 115 CTCGAG C^T[CT][GA]AG
SphI 791 GCATGC GCATG^C
SspI 306 AATATT AAT^ATT
StuI 172 AGGCCT AGG^CCT
Van91I 35 CCATGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 115 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AccI 723 GTCGAC GT^[AC][GT]AC
1150 GTAGAC
AlwNI 473 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1190 CAGGTGCTG
AspHI 565 GTGCTC G[AT]GC[AT]^C
816 GAGCTC
AvaI 115 CTCGAG C^[CT]CG[GA]G
631 CCCGAG
BamHI 140 GGATCC G^GATCC
468 GGATCC
BsaWI 449 TCCGGA [AT]^CCGG[AT]
713 TCCGGT
BsiHKAI 565 GTGCTC G[AT]GC[AT]^C
816 GAGCTC
BsmAI 111 GTCTC GTCTC(1/5)
334 GTCTC
BsoBI 115 CTCGAG C^[CT]CG[GA]G
631 CCCGAG
BspMI 11 GCAGGT ACCTGC(4/8)
903 GCAGGT
BstAPI 1000 GCAAATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1102 GCAACAACTGC
HapII 450 CCGG C^CGG
714 CCGG
HgaI 75 GACGC GACGC(5/10)
211 GCGTC
HinfI 487 GATTC G^A[ACGT]TC
988 GATTC
HpaII 450 CCGG C^CGG
714 CCGG
MspI 450 CCGG C^CGG
714 CCGG
NcoI 201 CCATGG C^CATGG
269 CCATGG
NspI 791 GCATGC [GA]CATG^[CT]
980 ACATGT
TfiI 487 GATTC G^A[AT]TC
988 GATTC
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BmyI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DpnII DsaI EcoRII
EcoT14I Fnu4HI FokI HaeIII HphI
ItaI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII