Restriction map for
pFN21AE2573
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AocI
ApoI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BciVI
BclI BfrI BlnI BsaBI BsaI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BsrDI BsrGI Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eam1105I Eco81I EcoNI
EcoRI EcoRV FbaI FspI HincII
HindII HpaI KpnI KspI MamI
MfeI MluI MroI MseI MunI
NdeI NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TfiI Tru9I XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AflIII 995 ACATGT A^C[GA][CT]GT
Aor51HI 2186 AGCGCT AGC^GCT
AscI 174 GGCGCGCC GG^CGCGCC
AspI 805 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1705 CACGTG CAC^GTG
BbsI 1684 GTCTTC GAAGAC(2/6)
BbvCI 645 CCTCAGC CCTCAGC(-5/-2)
BcgI 800 GCAACGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1484 AGATCT A^GATCT
BpuAI 1684 GTCTTC GAAGAC(2/6)
BsmBI 1519 CGTCTC CGTCTC(1/5)
BsmI 845 GCATTC GAATGC(1/-1)
BspLU11I 995 ACATGT A^CATGT
BssSI 883 CACGAG CACGAG(-5/-1)
BstEII 877 GGTGACC G^GT[ACGT]ACC
BstPI 877 GGTGACC G^GT[ACGT]ACC
EarI 2092 GAAGAG CTCTTC(1/4)
Eco47III 2186 AGCGCT AGC^GCT
EcoO65I 877 GGTGACC G^GT[ACGT]ACC
EcoT14I 1747 CCATGG C^C[AT][AT]GG
FseI 679 GGCCGGCC GGCCGG^CC
HindIII 1013 AAGCTT A^AGCTT
HinfI 139 GACTC G^A[ACGT]TC
Ksp632I 2092 GAAGAG CTCTTC(1/4)
NcoI 1747 CCATGG C^CATGG
PflFI 805 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 139 GACTC GAGTC(4/5)
PmaCI 1705 CACGTG CAC^GTG
PmlI 1705 CACGTG CAC^GTG
SfiI 32 GGCCCGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 2257 CTTGAG C^T[CT][GA]AG
StyI 1747 CCATGG C^C[AT][AT]GG
TaqI 851 TCGA T^CGA
Tsp509I 247 AATT ^AATT
Tth111I 805 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 851 TCGA T^CGA
XbaI 1287 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 235 GGCGCC G[GA]^CG[CT]C
2066 GGCGCC
Alw44I 892 GTGCAC G^TGCAC
991 GTGCAC
AlwNI 349 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1336 CAGCAGCTG
ApaLI 892 GTGCAC G^TGCAC
991 GTGCAC
BalI 639 TGGCCA TGG^CCA
2285 TGGCCA
BbeI 235 GGCGCC GGCGC^C
2066 GGCGCC
BfaI 1288 CTAG C^TAG
1540 CTAG
BsaHI 235 GGCGCC G[GA]^CG[CT]C
2066 GGCGCC
BsiEI 717 CGGCCG CG[GA][CT]^CG
1465 CGGCCG
BsmAI 137 GAGAC GTCTC(1/5)
1520 GTCTC
BspMI 527 ACCTGC ACCTGC(4/8)
548 ACCTGC
BsrBI 203 GAGCGG CCGCTC(-3/-3)
1720 GAGCGG
BssHII 175 GCGCGC G^CGCGC
817 GCGCGC
BstF5I 1145 CATCC GGATG(2/0)
2115 GGATG
BstXI 857 CCACCGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1085 CCAGCCATCTGG
DraIII 973 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1238 CACTGCGTG
EagI 717 CGGCCG C^GGCCG
1465 CGGCCG
EclXI 717 CGGCCG C^GGCCG
1465 CGGCCG
Eco52I 717 CGGCCG C^GGCCG
1465 CGGCCG
EcoT22I 1888 ATGCAT ATGCA^T
1924 ATGCAT
FokI 1145 CATCC GGATG(9/13)
2115 GGATG
Hin1I 235 GGCGCC G[GA]^CG[CT]C
2066 GGCGCC
KasI 235 GGCGCC G^GCGCC
2066 GGCGCC
MaeI 1288 CTAG C^TAG
1540 CTAG
MluNI 639 TGGCCA TGG^CCA
2285 TGGCCA
MscI 639 TGGCCA TGG^CCA
2285 TGGCCA
NarI 235 GGCGCC GG^CGCC
2066 GGCGCC
NsiI 1888 ATGCAT ATGCA^T
1924 ATGCAT
PstI 1592 CTGCAG CTGCA^G
2182 CTGCAG
SfoI 235 GGCGCC GGC^GCC
2066 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
ApaI AspHI AvaI AvaII BanI
BanII BbvI BcnI BglI BlpI
BmyI BpmI Bpu1102I BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstAPI BstNI BstUI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SphI StuI TseI
Tsp45I TspRI Van91I XcmI XhoII
XmaCI XmaI