Restriction map for
pFN21AE2613
##################################
# NO CUT ENZYMES ( 132 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BbeI BbrPI BcgI BciVI BclI
BfaI BfrI BlnI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmI
BspDI BspEI BspHI BspLU11I BsrBI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I ClaI
CpoI DraI DraII DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV FbaI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI HphI
KasI KspI MaeI MfeI MluI
MroI MseI MunI MvnI NarI
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SpeI
Sse8387I SspI StuI SwaI TfiI
Tru9I Tsp509I Tth111I XbaI XcmI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 1100 GGTACC G^GTACC
Asp718I 1100 GGTACC G^GTACC
AspHI 157 GAGCTC G[AT]GC[AT]^C
AvaII 3 GGACC G^G[AT]CC
BalI 386 TGGCCA TGG^CCA
BamHI 101 GGATCC G^GATCC
BglII 539 AGATCT A^GATCT
BsaAI 241 TACGTA [CT]AC^GT[GA]
BsaBI 511 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 780 CTGCAC GTGCAG(16/14)
BsiHKAI 157 GAGCTC G[AT]GC[AT]^C
BsmBI 869 CGTCTC CGTCTC(1/5)
Bsp1407I 302 TGTACA T^GTACA
BspMI 1042 ACCTGC ACCTGC(4/8)
BsrFI 49 GCCGGC [GA]^CCGG[CT]
BsrGI 302 TGTACA T^GTACA
BstAPI 175 GCAGACATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 353 CATCC GGATG(2/0)
CfoI 1084 GCGC GCG^C
Cfr10I 49 GCCGGC [GA]^CCGG[CT]
DrdI 178 GACATTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 506 CCATGG C^C[GA][CT]GG
EarI 992 CTCTTC CTCTTC(1/4)
EcoNI 482 CCTTGGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 267 ATGCAT ATGCA^T
FauI 874 CCCGC CCCGC(4/6)
FokI 353 CATCC GGATG(9/13)
HhaI 1084 GCGC GCG^C
HinP1I 1084 GCGC G^CGC
KpnI 1100 GGTACC GGTAC^C
Ksp632I 992 CTCTTC CTCTTC(1/4)
MaeIII 918 GTCAC ^GT[ACGT]AC
MamI 511 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 386 TGGCCA TGG^CCA
MscI 386 TGGCCA TGG^CCA
NaeI 49 GCCGGC GCC^GGC
NcoI 506 CCATGG C^CATGG
NdeI 713 CATATG CA^TATG
NgoMIV 49 GCCGGC G^CCGGC
NsiI 267 ATGCAT ATGCA^T
PvuII 888 CAGCTG CAG^CTG
SacI 157 GAGCTC GAGCT^C
SfcI 144 CTGTAG C^T[GA][CT]AG
SmaI 257 CCCGGG CCC^GGG
SmlI 1207 CTCAAG C^T[CT][GA]AG
SnaBI 241 TACGTA TAC^GTA
SphI 265 GCATGC GCATG^C
SspBI 302 TGTACA T^GTACA
TaqI 188 TCGA T^CGA
Tsp45I 918 GTCAC ^GT[GC]AC
TthHB8I 188 TCGA T^CGA
XmaCI 257 CCCGGG C^CCGGG
XmaI 257 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AlwI 102 GATCC GGATC(4/5)
101 GGATC
AvaI 257 CCCGGG C^[CT]CG[GA]G
533 CTCGGG
BbsI 427 GTCTTC GAAGAC(2/6)
932 GTCTTC
BbvCI 1159 CCTCAGC CCTCAGC(-5/-2)
1165 CCTCAGC
BglI 642 GCCGACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
893 GCCTGGAAGGC
BlpI 552 GCTGAGC GC^T[ACGT]AGC
577 GCTGAGC
Bpu1102I 552 GCTGAGC GC^T[ACGT]AGC
577 GCTGAGC
BpuAI 427 GTCTTC GAAGAC(2/6)
932 GTCTTC
BsoBI 257 CCCGGG C^[CT]CG[GA]G
533 CTCGGG
BsrI 131 ACTGG ACTGG(1/-1)
389 CCAGT
BstYI 101 GGATCC [GA]^GATC[CT]
539 AGATCT
CelII 552 GCTGAGC GC^T[ACGT]AGC
577 GCTGAGC
MaeII 67 ACGT A^CGT
242 ACGT
MflI 101 GGATCC [GA]^GATC[CT]
539 AGATCT
MslI 378 CATGATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1105 CATACCTATG
MspA1I 676 CAGCGG C[AC]G^C[GT]G
888 CAGCTG
NspI 265 GCATGC [GA]CATG^[CT]
345 GCATGT
XhoII 101 GGATCC [GA]^GATC[CT]
539 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AfaI AluI AlwNI BanI
BanII BbvI BcnI BmyI BpmI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BssKI BstNI
BstXI Cac8I Cfr13I DdeI DpnII
EaeI EcoRII EcoT14I Fnu4HI HaeIII
HapII HinfI HpaII ItaI MboI
MboII MnlI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TseI TspRI Van91I