Restriction map for
pFN21AE2220
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# NO CUT ENZYMES ( 173 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspLU11I BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI Ksp632I KspI MaeI MaeIII
MamI MfeI MluI MluNI MroI
MscI MseI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI TfiI Tru9I Tsp45I Tsp509I
Tth111I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AlwI 360 GATCC GGATC(4/5)
AlwNI 90 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
BanI 458 GGTGCC G^G[CT][GA]CC
BbrPI 373 CACGTG CAC^GTG
BpmI 379 CTGGAG CTGGAG(16/14)
BsaAI 373 CACGTG [CT]AC^GT[GA]
BsmFI 143 GTCCC GGGAC(10/14)
BspHI 302 TCATGA T^CATGA
BsrDI 201 GCAATG GCAATG(2/0)
BsrI 467 ACTGG ACTGG(1/-1)
BstAPI 426 GCAATACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 104 CCATCCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 359 AGATCC [GA]^GATC[CT]
Cac8I 490 GCAGGC GC[ACGT]^[ACGT]GC
DsaI 476 CCATGG C^C[GA][CT]GG
HgaI 141 GCGTC GACGC(5/10)
HincII 158 GTCAAC GT[CT]^[GA]AC
HindII 158 GTCAAC GT[CT]^[GA]AC
HinfI 220 GACTC G^A[ACGT]TC
HphI 11 TCACC GGTGA(8/7)
MaeII 374 ACGT A^CGT
MflI 359 AGATCC [GA]^GATC[CT]
MslI 105 CATCCGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 93 CCGCTG C[AC]G^C[GT]G
NcoI 476 CCATGG C^CATGG
PflMI 372 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 220 GACTC GAGTC(4/5)
PmaCI 373 CACGTG CAC^GTG
PmlI 373 CACGTG CAC^GTG
PpuMI 392 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 76 CTGCAG CTGCA^G
RcaI 302 TCATGA T^CATGA
SfcI 76 CTGCAG C^T[GA][CT]AG
StuI 297 AGGCCT AGG^CCT
TspRI 60 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 372 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 359 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AvaII 339 GGACC G^G[AT]CC
393 GGTCC
BbsI 286 GAAGAC GAAGAC(2/6)
319 GAAGAC
BcnI 108 CCGGG CC^[GC]GG
417 CCGGG
BpuAI 286 GAAGAC GAAGAC(2/6)
319 GAAGAC
BsmAI 239 GTCTC GTCTC(1/5)
256 GTCTC
BspMI 74 ACCTGC ACCTGC(4/8)
431 ACCTGC
BsrBI 228 CCGCTC CCGCTC(-3/-3)
117 GAGCGG
DpnII 9 GATC ^GATC
360 GATC
EcoT14I 153 CCAAGG C^C[AT][AT]GG
476 CCATGG
HapII 108 CCGG C^CGG
417 CCGG
HpaII 108 CCGG C^CGG
417 CCGG
MboI 9 GATC ^GATC
360 GATC
MspI 108 CCGG C^CGG
417 CCGG
NciI 108 CCGGG CC^[GC]GG
417 CCGGG
NdeII 9 GATC ^GATC
360 GATC
NlaIII 303 CATG CATG^
477 CATG
Sau3AI 9 GATC ^GATC
360 GATC
StyI 153 CCAAGG C^C[AT][AT]GG
476 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 30 enzymes) #
#########################################
AciI AfaI AluI BbvCI BbvI
BsaJI BsiYI BslI BssKI BstF5I
BstNI Cfr13I DdeI DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII ItaI
MboII MnlI MvaI MwoI NlaIV
RsaI Sau96I ScrFI SfaNI TseI