Restriction map for
pFN21AE6527
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI DraI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I MaeI MaeIII MamI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI SacI
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI TfiI
Tru9I Tsp45I Tsp509I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AluI 404 AGCT AG^CT
AlwI 266 GGATC GGATC(4/5)
AspI 220 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 153 CTCGGG C^[CT]CG[GA]G
BbsI 332 GTCTTC GAAGAC(2/6)
BbvCI 213 CCTCAGC CCTCAGC(-5/-2)
BcnI 27 CCGGG CC^[GC]GG
BpuAI 332 GTCTTC GAAGAC(2/6)
BseRI 501 GAGGAG GAGGAG(10/8)
BsmFI 446 GGGAC GGGAC(10/14)
BsoBI 153 CTCGGG C^[CT]CG[GA]G
BsrBI 228 CCGCTC CCGCTC(-3/-3)
BssHII 106 GCGCGC G^CGCGC
BstAPI 426 GCAGTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 106 GCGCGC GC[ACGT]^[ACGT]GC
CpoI 224 CGGTCCG CG^G[AT]CCG
DraII 392 GGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 177 CCGCGG C^C[GA][CT]GG
EcoNI 15 CCTCTACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 392 GGGTCCT [GA]G^G[ACGT]CC[CT]
HaeII 455 GGCGCT [GA]GCGC^[CT]
HapII 27 CCGG C^CGG
HpaII 27 CCGG C^CGG
HphI 11 TCACC GGTGA(8/7)
KspI 177 CCGCGG CCGC^GG
MaeII 374 ACGT A^CGT
MspI 27 CCGG C^CGG
NciI 27 CCGGG CC^[GC]GG
PflFI 220 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 392 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 76 CTGCAG CTGCA^G
RsrII 224 CGGTCCG CG^G[AT]CCG
SacII 177 CCGCGG CCGC^GG
ScaI 164 AGTACT AGT^ACT
SmlI 87 CTTGAG C^T[CT][GA]AG
Tth111I 220 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 17 enzymes) #
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AfaI 165 GTAC GT^AC
429 GTAC
AlwNI 117 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
427 CAGTACCTG
BanII 384 GGGCTC G[GA]GC[CT]^C
492 GGGCTC
BmyI 384 GGGCTC G[AGT]GC[ACT]^C
492 GGGCTC
BpmI 160 CTCCAG CTGGAG(16/14)
379 CTGGAG
BsmI 52 GAATGC GAATGC(1/-1)
480 GAATGC
Bsp1286I 384 GGGCTC G[AGT]GC[ACT]^C
492 GGGCTC
BspMI 74 ACCTGC ACCTGC(4/8)
431 ACCTGC
BsrI 467 ACTGG ACTGG(1/-1)
162 CCAGT
DpnII 9 GATC ^GATC
267 GATC
MboI 9 GATC ^GATC
267 GATC
MboII 6 GAAGA GAAGA(8/7)
333 TCTTC
NdeII 9 GATC ^GATC
267 GATC
RsaI 165 GTAC GT^AC
429 GTAC
Sau3AI 9 GATC ^GATC
267 GATC
SfaNI 129 GATGC GCATC(5/9)
438 GATGC
TspRI 202 CAGTG CA[GC]TG[ACGT][ACGT]^
315 CACTG
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AccII AciI AvaII BbvI BsaJI
BsiYI BslI BssKI BstF5I BstNI
BstUI CfoI Cfr13I DdeI EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI ItaI MnlI MspA1I MvaI
MvnI MwoI NlaIV PleI Sau96I
ScrFI SfcI TseI