Restriction map for
pFN21AE9517
##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI AlwNI AocI
ApoI AscI AseI AsnI Asp718I
AspEI AspI AvrII BamHI BbvCI
BfrI BlnI BsaBI BsaI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII Eam1105I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FseI HincII HindII
HindIII HinfI HpaI KpnI MaeIII
MamI MfeI MluI MroI MunI
NdeI NheI NruI NsiI NspV
PacI PflFI PinAI PleI PmeI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SalI SapI SbfI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Tsp45I Tsp509I Tth111I
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AflIII 491 ACACGT A^C[GA][CT]GT
AlwI 1640 GGATC GGATC(4/5)
Aor51HI 950 AGCGCT AGC^GCT
ApaI 510 GGGCCC GGGCC^C
Asp700I 405 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1406 TGCGCA TGC^GCA
BbrPI 907 CACGTG CAC^GTG
BciVI 1346 GTATCC GTATCC(6/5)
BclI 1592 TGATCA T^GATCA
BfaI 925 CTAG C^TAG
BglII 473 AGATCT A^GATCT
BlpI 1218 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1218 GCTGAGC GC^T[ACGT]AGC
BsaAI 907 CACGTG [CT]AC^GT[GA]
BssHII 375 GCGCGC G^CGCGC
BssSI 1111 CACGAG CACGAG(-5/-1)
CelII 1218 GCTGAGC GC^T[ACGT]AGC
Eco47III 950 AGCGCT AGC^GCT
EcoNI 921 CCTTCTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1193 CCATGG C^C[AT][AT]GG
FbaI 1592 TGATCA T^GATCA
FspI 1406 TGCGCA TGC^GCA
KspI 80 CCGCGG CCGC^GG
MaeI 925 CTAG C^TAG
MseI 734 TTAA T^TAA
NcoI 1193 CCATGG C^CATGG
NotI 16 GCGGCCGC GC^GGCCGC
NspI 833 ACATGC [GA]CATG^[CT]
PaeR7I 241 CTCGAG C^TCGAG
PflMI 1052 CCATCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 907 CACGTG CAC^GTG
PmlI 907 CACGTG CAC^GTG
SacII 80 CCGCGG CCGC^GG
ScaI 1136 AGTACT AGT^ACT
SexAI 824 ACCTGGT A^CC[AT]GGT
SmaI 582 CCCGGG CCC^GGG
StuI 1478 AGGCCT AGG^CCT
StyI 1193 CCATGG C^C[AT][AT]GG
Tru9I 734 TTAA T^TAA
Van91I 1052 CCATCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 924 TCTAGA T^CTAGA
XhoI 241 CTCGAG C^TCGAG
XmaCI 582 CCCGGG C^CCGGG
XmaI 582 CCCGGG C^CCGGG
XmnI 405 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 1204 GTAGAC GT^[AC][GT]AC
1291 GTCTAC
BalI 1043 TGGCCA TGG^CCA
1190 TGGCCA
BanII 510 GGGCCC G[GA]GC[CT]^C
1569 GAGCCC
BbsI 1417 GTCTTC GAAGAC(2/6)
1585 GTCTTC
BcgI 170 GCACGGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
329 GCACCGTGCTCG
BpmI 1245 CTCCAG CTGGAG(16/14)
1390 CTGGAG
BpuAI 1417 GTCTTC GAAGAC(2/6)
1585 GTCTTC
BseRI 739 GAGGAG GAGGAG(10/8)
742 GAGGAG
BsgI 1005 CTGCAC GTGCAG(16/14)
274 GTGCAG
BsiEI 17 CGGCCG CG[GA][CT]^CG
1091 CGGCCG
BsmAI 1711 GAGAC GTCTC(1/5)
1071 GTCTC
BstXI 203 CCAACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1448 CCATCGTCCTGG
BstYI 473 AGATCT [GA]^GATC[CT]
1640 GGATCT
DrdI 1576 GACTTCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1656 GACCCTGCAGTC
EagI 17 CGGCCG C^GGCCG
1091 CGGCCG
EclXI 17 CGGCCG C^GGCCG
1091 CGGCCG
Eco52I 17 CGGCCG C^GGCCG
1091 CGGCCG
MflI 473 AGATCT [GA]^GATC[CT]
1640 GGATCT
MluNI 1043 TGGCCA TGG^CCA
1190 TGGCCA
MscI 1043 TGGCCA TGG^CCA
1190 TGGCCA
MslI 120 CATCTCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1353 CACCATCATG
PpuMI 5 GGGACCC [GA]G^G[AT]CC[CT]
1211 GGGACCT
PstI 1513 CTGCAG CTGCA^G
1660 CTGCAG
SfcI 1513 CTGCAG C^T[GA][CT]AG
1660 CTGCAG
SfiI 1116 GGCCATCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1149 GGCCGCGTGGGCC
SmlI 241 CTCGAG C^T[CT][GA]AG
640 CTCAAG
XhoII 473 AGATCT [GA]^GATC[CT]
1640 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I ApaLI AspHI AvaI AvaII
BanI BbeI BbvI BcnI BglI
BmyI BsaHI BsaJI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspHI
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI KasI Ksp632I
MaeII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NdeII NgoMIV NlaIII
NlaIV RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfoI TaqI TseI
TspRI TthHB8I XcmI