Restriction map for
pFN21AB6641
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII Acc65I AccII AccIII AciI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbsI
BbvCI BcgI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshBI PstI
PvuI RcaI RsrII SacII SalI
SapI SbfI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 922 GTAGAC GT^[AC][GT]AC
AclI 160 AACGTT AA^CGTT
AlwI 1057 GATCC GGATC(4/5)
AspHI 175 GAGCTC G[AT]GC[AT]^C
BanI 795 GGTGCC G^G[CT][GA]CC
BanII 175 GAGCTC G[GA]GC[CT]^C
BciVI 77 GTATCC GTATCC(6/5)
BmyI 175 GAGCTC G[AGT]GC[ACT]^C
BsiHKAI 175 GAGCTC G[AT]GC[AT]^C
Bsp1286I 175 GAGCTC G[AGT]GC[ACT]^C
BspMI 792 GCAGGT ACCTGC(4/8)
BstYI 1056 AGATCC [GA]^GATC[CT]
DpnII 1057 GATC ^GATC
EarI 194 GAAGAG CTCTTC(1/4)
EcoRI 1249 GAATTC G^AATTC
EcoRV 868 GATATC GAT^ATC
EcoT14I 1198 CCTTGG C^C[AT][AT]GG
EcoT22I 811 ATGCAT ATGCA^T
HapII 342 CCGG C^CGG
HincII 434 GTCAAC GT[CT]^[GA]AC
HindII 434 GTCAAC GT[CT]^[GA]AC
HindIII 305 AAGCTT A^AGCTT
HpaII 342 CCGG C^CGG
HphI 580 GGTGA GGTGA(8/7)
Ksp632I 194 GAAGAG CTCTTC(1/4)
MboI 1057 GATC ^GATC
MflI 1056 AGATCC [GA]^GATC[CT]
MspA1I 387 CAGCTG C[AC]G^C[GT]G
MspI 342 CCGG C^CGG
NdeII 1057 GATC ^GATC
NsiI 811 ATGCAT ATGCA^T
PshAI 213 GACTTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 160 AACGTT AA^CGTT
PvuII 387 CAGCTG CAG^CTG
SacI 175 GAGCTC GAGCT^C
Sau3AI 1057 GATC ^GATC
ScaI 144 AGTACT AGT^ACT
SmlI 441 CTTGAG C^T[CT][GA]AG
SspI 352 AATATT AAT^ATT
StyI 1198 CCTTGG C^C[AT][AT]GG
XhoII 1056 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 906 AAATTC [GA]^AATT[CT]
1249 GAATTC
AfaI 145 GTAC GT^AC
1036 GTAC
AlwNI 823 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
835 CAGAAACTG
ApoI 906 AAATTC [GA]^AATT[CT]
1249 GAATTC
AvaII 446 GGACC G^G[AT]CC
847 GGTCC
BbvI 386 GCAGC GCAGC(8/12)
687 GCAGC
BfaI 131 CTAG C^TAG
589 CTAG
BpmI 954 CTCCAG CTGGAG(16/14)
1187 CTCCAG
BsiYI 107 CCCTTTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
503 CCGTGAGAGGG
BslI 107 CCCTTTCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
503 CCGTGAGAGGG
BsmFI 609 GTCCC GGGAC(10/14)
1092 GTCCC
BsrDI 1181 CATTGC GCAATG(2/0)
287 GCAATG
Cfr13I 446 GGACC G^G[ACGT]CC
847 GGTCC
DraII 445 AGGACCT [GA]G^G[ACGT]CC[CT]
846 AGGTCCT
DsaI 242 CCACGG C^C[GA][CT]GG
691 CCGTGG
EcoO109I 445 AGGACCT [GA]G^G[ACGT]CC[CT]
846 AGGTCCT
Fnu4HI 386 GCAGC GC^[ACGT]GC
687 GCAGC
ItaI 386 GCAGC GC^[ACGT]GC
687 GCAGC
MaeI 131 CTAG C^TAG
589 CTAG
MaeII 120 ACGT A^CGT
161 ACGT
MslI 526 CACCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
678 CACATCAGTG
MwoI 687 GCAGCCGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1041 GCTCTGTTTGC
NlaIV 499 GGAGCC GG[ACGT]^[ACGT]CC
795 GGTGCC
NspI 772 GCATGT [GA]CATG^[CT]
809 ACATGC
PpuMI 445 AGGACCT [GA]G^G[AT]CC[CT]
846 AGGTCCT
RsaI 145 GTAC GT^AC
1036 GTAC
Sau96I 446 GGACC G^G[ACGT]CC
847 GGTCC
SfaNI 825 GATGC GCATC(5/9)
345 GCATC
TseI 386 GCAGC G^C[AT]GC
687 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AluI BsaJI BsgI BsmAI BsrI
BssKI BstF5I BstNI Cac8I DdeI
EcoRII FokI HaeIII HinfI MaeIII
MboII MnlI MseI MvaI NlaIII
PleI ScrFI TfiI Tru9I Tsp45I
Tsp509I TspRI XcmI