Restriction map for
pFN21AE5243
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# NO CUT ENZYMES ( 186 enzymes) #
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AatII Acc65I AccI AccIII AciI
AclI AcyI AflII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsiYI BslI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DdeI DraI
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I FauI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI Ksp632I KspI MaeII MamI
MfeI MflI MluNI MroI MscI
MspA1I MunI MwoI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccII 94 CGCG CG^CG
AflIII 93 ACGCGT A^C[GA][CT]GT
AluI 546 AGCT AG^CT
BbvI 155 GCAGC GCAGC(8/12)
BfaI 548 CTAG C^TAG
BmyI 377 GGGCAC G[AGT]GC[ACT]^C
BpmI 82 CTGGAG CTGGAG(16/14)
Bsp1286I 377 GGGCAC G[AGT]GC[ACT]^C
BsrDI 506 GCAATG GCAATG(2/0)
BstUI 94 CGCG CG^CG
Cac8I 496 GCTGGC GC[ACGT]^[ACGT]GC
DraII 587 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 380 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 587 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 233 ATGCAT ATGCA^T
Fnu4HI 155 GCAGC GC^[ACGT]GC
HgaI 92 GACGC GACGC(5/10)
HinfI 469 GAATC G^A[ACGT]TC
HphI 280 GGTGA GGTGA(8/7)
ItaI 155 GCAGC GC^[ACGT]GC
MaeI 548 CTAG C^TAG
MaeIII 97 GTGAC ^GT[ACGT]AC
MboII 393 GAAGA GAAGA(8/7)
MluI 93 ACGCGT A^CGCGT
MslI 236 CATCCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 94 CGCG CG^CG
NsiI 233 ATGCAT ATGCA^T
NspI 492 ACATGC [GA]CATG^[CT]
PpuMI 587 GGGACCC [GA]G^G[AT]CC[CT]
TaqI 357 TCGA T^CGA
TfiI 469 GAATC G^A[AT]TC
TseI 155 GCAGC G^C[AT]GC
Tsp45I 97 GTGAC ^GT[GC]AC
TthHB8I 357 TCGA T^CGA
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 301 GAATTT [GA]^AATT[CT]
330 AAATTT
AfaI 422 GTAC GT^AC
428 GTAC
ApoI 301 GAATTT [GA]^AATT[CT]
330 AAATTT
AvaII 436 GGACC G^G[AT]CC
588 GGACC
BcnI 46 CCCGG CC^[GC]GG
513 CCGGG
BsmFI 516 GGGAC GGGAC(10/14)
587 GGGAC
BstNI 81 CCTGG CC^[AT]GG
202 CCAGG
DpnII 128 GATC ^GATC
183 GATC
EaeI 114 CGGCCA [CT]^GGCC[GA]
510 TGGCCG
EcoRII 81 CCTGG ^CC[AT]GG
202 CCAGG
HapII 47 CCGG C^CGG
513 CCGG
HincII 10 GTCAAC GT[CT]^[GA]AC
219 GTTGAC
HindII 10 GTCAAC GT[CT]^[GA]AC
219 GTTGAC
HpaII 47 CCGG C^CGG
513 CCGG
MboI 128 GATC ^GATC
183 GATC
MspI 47 CCGG C^CGG
513 CCGG
MvaI 81 CCTGG CC^[AT]GG
202 CCAGG
NciI 46 CCCGG CC^[GC]GG
513 CCGGG
NdeII 128 GATC ^GATC
183 GATC
NlaIV 587 GGGACC GG[ACGT]^[ACGT]CC
588 GGACCC
RsaI 422 GTAC GT^AC
428 GTAC
Sau3AI 128 GATC ^GATC
183 GATC
SfaNI 158 GCATC GCATC(5/9)
235 GCATC
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# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
BsaJI BsrI BssKI BstF5I Cfr13I
FokI HaeIII MnlI MseI NlaIII
Sau96I ScrFI Tru9I Tsp509I TspRI