Restriction map for
pFN21AE5429
##################################
# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BbrPI BbsI BbvCI BcgI BciVI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BseAI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraII
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV FseI
FspI HindIII HpaI KpnI KspI
MamI MluI MluNI MroI MscI
NciI NdeI NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 497 GTCGAC GT^[AC][GT]AC
AccII 972 CGCG CG^CG
AlwNI 976 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
BamHI 868 GGATCC G^GATCC
BanI 6 GGCGCC G^G[CT][GA]CC
BanII 671 GGGCTC G[GA]GC[CT]^C
BbeI 6 GGCGCC GGCGC^C
BclI 956 TGATCA T^GATCA
BpmI 889 CTGGAG CTGGAG(16/14)
BsaI 909 GGTCTC GGTCTC(1/5)
BsaWI 186 TCCGGT [AT]^CCGG[AT]
BsmFI 750 GGGAC GGGAC(10/14)
BsmI 838 GCATTC GAATGC(1/-1)
BspMI 23 ACCTGC ACCTGC(4/8)
BsrDI 590 GCAATG GCAATG(2/0)
BsrFI 162 GCCGGC [GA]^CCGG[CT]
BssHII 971 GCGCGC G^CGCGC
BstUI 972 CGCG CG^CG
BstXI 784 CCAGAAATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 162 GCCGGC [GA]^CCGG[CT]
Cfr13I 309 GGCCC G^G[ACGT]CC
DdeI 194 CTAAG C^T[ACGT]AG
EcoT22I 896 ATGCAT ATGCA^T
FauI 311 CCCGC CCCGC(4/6)
FbaI 956 TGATCA T^GATCA
HaeII 6 GGCGCC [GA]GCGC^[CT]
HgaI 17 GCGTC GACGC(5/10)
HinfI 279 GATTC G^A[ACGT]TC
KasI 6 GGCGCC G^GCGCC
MfeI 650 CAATTG C^AATTG
MslI 715 CATCAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 304 CAGCTG C[AC]G^C[GT]G
MunI 650 CAATTG C^AATTG
MvnI 972 CGCG CG^CG
NaeI 162 GCCGGC GCC^GGC
NarI 6 GGCGCC GG^CGCC
NgoMIV 162 GCCGGC G^CCGGC
NsiI 896 ATGCAT ATGCA^T
PshAI 903 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 167 CTGCAG CTGCA^G
PvuII 304 CAGCTG CAG^CTG
SalI 497 GTCGAC G^TCGAC
Sau96I 309 GGCCC G^G[ACGT]CC
SfoI 6 GGCGCC GGC^GCC
TfiI 279 GATTC G^A[AT]TC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcyI 6 GGCGCC G[GA]^CG[CT]C
16 GGCGTC
AfaI 235 GTAC GT^AC
455 GTAC
AspHI 472 GTGCTC G[AT]GC[AT]^C
943 GAGCAC
BbvI 655 GCAGC GCAGC(8/12)
166 GCTGC
BfaI 424 CTAG C^TAG
916 CTAG
BsaHI 6 GGCGCC G[GA]^CG[CT]C
16 GGCGTC
BsaJI 558 CCATGG C^C[ACGT][ACGT]GG
821 CCATGG
BseRI 322 CTCCTC GAGGAG(10/8)
826 GAGGAG
BsiHKAI 472 GTGCTC G[AT]GC[AT]^C
943 GAGCAC
BsiYI 238 CCAGACTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
321 CCTCCTCTTGG
BslI 238 CCAGACTGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
321 CCTCCTCTTGG
BsrI 242 ACTGG ACTGG(1/-1)
11 CCAGT
BssKI 629 CCAGG ^CC[ACGT]GG
888 CCTGG
BstNI 629 CCAGG CC^[AT]GG
888 CCTGG
DraI 342 TTTAAA TTT^AAA
880 TTTAAA
DsaI 558 CCATGG C^C[GA][CT]GG
821 CCATGG
EcoRII 629 CCAGG ^CC[AT]GG
888 CCTGG
EcoT14I 558 CCATGG C^C[AT][AT]GG
821 CCATGG
HaeIII 309 GGCC GG^CC
1002 GGCC
HapII 163 CCGG C^CGG
187 CCGG
Hin1I 6 GGCGCC G[GA]^CG[CT]C
16 GGCGTC
HpaII 163 CCGG C^CGG
187 CCGG
HphI 61 TCACC GGTGA(8/7)
418 TCACC
MaeI 424 CTAG C^TAG
916 CTAG
MaeIII 220 GTGAC ^GT[ACGT]AC
535 GTCAC
MspI 163 CCGG C^CGG
187 CCGG
MvaI 629 CCAGG CC^[AT]GG
888 CCTGG
NcoI 558 CCATGG C^CATGG
821 CCATGG
RsaI 235 GTAC GT^AC
455 GTAC
ScrFI 629 CCAGG CC^[ACGT]GG
888 CCTGG
SfcI 167 CTGCAG C^T[GA][CT]AG
845 CTATAG
StyI 558 CCATGG C^C[AT][AT]GG
821 CCATGG
TseI 166 GCTGC G^C[AT]GC
655 GCAGC
Tsp45I 220 GTGAC ^GT[GC]AC
535 GTCAC
TspRI 12 CAGTG CA[GC]TG[ACGT][ACGT]^
810 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BmyI BsmAI Bsp1286I BstF5I BstYI
Cac8I CfoI DpnII EarI Fnu4HI
FokI HhaI HinP1I HincII HindII
ItaI Ksp632I MaeII MboI MboII
MflI MnlI MseI MwoI NdeII
NlaIII NlaIV Sau3AI SfaNI TaqI
Tru9I Tsp509I TthHB8I XhoII