Restriction map for
pFN21AE2081
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmI
BsoBI BspDI BspEI BspLU11I BsrBI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MfeI MluI
MroI MspA1I MunI NaeI NarI
NcoI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacII SalI
SapI SbfI ScaI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 28 enzymes) #
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AccI 807 GTCTAC GT^[AC][GT]AC
AccII 1621 CGCG CG^CG
BalI 335 TGGCCA TGG^CCA
BcnI 149 CCGGG CC^[GC]GG
BglII 982 AGATCT A^GATCT
BsmBI 963 GAGACG CGTCTC(1/5)
BspMI 57 ACCTGC ACCTGC(4/8)
BsrDI 39 CATTGC GCAATG(2/0)
BstAPI 1089 GCAAGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1621 CGCG CG^CG
CfoI 1622 GCGC GCG^C
EaeI 335 TGGCCA [CT]^GGCC[GA]
EcoT14I 1012 CCAAGG C^C[AT][AT]GG
HapII 149 CCGG C^CGG
HgaI 1619 GACGC GACGC(5/10)
HhaI 1622 GCGC GCG^C
HinP1I 1622 GCGC G^CGC
HpaII 149 CCGG C^CGG
MluNI 335 TGGCCA TGG^CCA
MscI 335 TGGCCA TGG^CCA
MspI 149 CCGG C^CGG
MvnI 1621 CGCG CG^CG
NciI 149 CCGGG CC^[GC]GG
NheI 484 GCTAGC G^CTAGC
NlaIV 145 GGGACC GG[ACGT]^[ACGT]CC
NspI 106 ACATGC [GA]CATG^[CT]
SmlI 618 CTCAAG C^T[CT][GA]AG
StyI 1012 CCAAGG C^C[AT][AT]GG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AciI 499 GCGG CCGC(-3/-1)
2258 GCGG
Asp700I 1025 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1316 GAAGAAATTC
AvaII 146 GGACC G^G[AT]CC
1009 GGACC
BbvI 481 GCAGC GCAGC(8/12)
1751 GCAGC
BciVI 351 GGATAC GTATCC(6/5)
509 GGATAC
BsaJI 1012 CCAAGG C^C[ACGT][ACGT]GG
2235 CCCTGG
BsiYI 338 CCAACAGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1040 CCAGATCAAGG
BslI 338 CCAACAGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1040 CCAGATCAAGG
Bsp1407I 130 TGTACA T^GTACA
2196 TGTACA
BspHI 1479 TCATGA T^CATGA
2223 TCATGA
BsrGI 130 TGTACA T^GTACA
2196 TGTACA
BsrI 804 CCAGT ACTGG(1/-1)
1192 CCAGT
BstF5I 1451 GGATG GGATG(2/0)
2130 GGATG
Cac8I 484 GCTAGC GC[ACGT]^[ACGT]GC
948 GCCTGC
Fnu4HI 481 GCAGC GC^[ACGT]GC
1751 GCAGC
FokI 1451 GGATG GGATG(9/13)
2130 GGATG
HaeIII 336 GGCC GG^CC
1365 GGCC
ItaI 481 GCAGC GC^[ACGT]GC
1751 GCAGC
MaeII 18 ACGT A^CGT
1062 ACGT
MwoI 1089 GCAAGAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1751 GCAGCAGAAGC
RcaI 1479 TCATGA T^CATGA
2223 TCATGA
SspBI 130 TGTACA T^GTACA
2196 TGTACA
TaqI 24 TCGA T^CGA
1023 TCGA
TseI 481 GCAGC G^C[AT]GC
1751 GCAGC
TspRI 237 CAGTG CA[GC]TG[ACGT][ACGT]^
2150 CAGTG
TthHB8I 24 TCGA T^CGA
1023 TCGA
XmnI 1025 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1316 GAAGAAATTC
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# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI BanII BbsI BclI BfaI
BmyI BpuAI BsaBI BseRI BsiHKAI
BsmAI BsmFI Bsp1286I BssKI BstNI
BstYI Cfr13I DdeI DpnII EarI
EcoRII FbaI HinfI HphI Ksp632I
MaeI MaeIII MamI MboI MboII
MflI MnlI MseI MslI MvaI
NdeII NlaIII PleI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI TfiI
Tru9I Tsp45I Tsp509I XhoII