Restriction map for
pFN21AE1769
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# NO CUT ENZYMES ( 169 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BanI BbeI
BbrPI BbvCI BcgI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiWI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII CfoI ClaI
CpoI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI HaeII HhaI
HinP1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MluI MluNI MroI
MscI MunI MvnI MwoI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AcyI 409 GGCGTC G[GA]^CG[CT]C
ApaI 298 GGGCCC GGGCC^C
BamHI 741 GGATCC G^GATCC
BanII 298 GGGCCC G[GA]GC[CT]^C
BbsI 37 GAAGAC GAAGAC(2/6)
BclI 2 TGATCA T^GATCA
BfaI 211 CTAG C^TAG
BmyI 298 GGGCCC G[AGT]GC[ACT]^C
BpuAI 37 GAAGAC GAAGAC(2/6)
BsaHI 409 GGCGTC G[GA]^CG[CT]C
BsiYI 301 CCCAACTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 301 CCCAACTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 347 GTCTC GTCTC(1/5)
BsmBI 346 CGTCTC CGTCTC(1/5)
Bsp1286I 298 GGGCCC G[AGT]GC[ACT]^C
BspHI 671 TCATGA T^CATGA
BsrDI 736 GCAATG GCAATG(2/0)
BsrFI 103 GCCGGT [GA]^CCGG[CT]
Cfr10I 103 GCCGGT [GA]^CCGG[CT]
DraI 436 TTTAAA TTT^AAA
DraII 297 AGGGCCC [GA]G^G[ACGT]CC[CT]
DsaI 606 CCATGG C^C[GA][CT]GG
EaeI 186 CGGCCA [CT]^GGCC[GA]
EcoO109I 297 AGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 606 CCATGG C^C[AT][AT]GG
FbaI 2 TGATCA T^GATCA
HapII 104 CCGG C^CGG
HgaI 410 GCGTC GACGC(5/10)
Hin1I 409 GGCGTC G[GA]^CG[CT]C
HpaII 104 CCGG C^CGG
MaeI 211 CTAG C^TAG
MslI 238 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 707 CAGCTG C[AC]G^C[GT]G
MspI 104 CCGG C^CGG
NcoI 606 CCATGG C^CATGG
NdeI 84 CATATG CA^TATG
PleI 497 GACTC GAGTC(4/5)
PvuII 707 CAGCTG CAG^CTG
RcaI 671 TCATGA T^CATGA
ScaI 778 AGTACT AGT^ACT
StyI 606 CCATGG C^C[AT][AT]GG
Tsp45I 703 GTGAC ^GT[GC]AC
TspRI 701 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 166 GCGG CCGC(-3/-1)
185 GCGG
BciVI 277 GGATAC GTATCC(6/5)
748 GTATCC
BpmI 443 CTCCAG CTGGAG(16/14)
710 CTGGAG
BstYI 420 AGATCC [GA]^GATC[CT]
741 GGATCC
Cfr13I 298 GGGCC G^G[ACGT]CC
299 GGCCC
HaeIII 187 GGCC GG^CC
299 GGCC
HphI 146 TCACC GGTGA(8/7)
519 TCACC
MaeIII 593 GTTAC ^GT[ACGT]AC
703 GTGAC
MflI 420 AGATCC [GA]^GATC[CT]
741 GGATCC
MnlI 214 GAGG CCTC(7/6)
713 GAGG
MseI 10 TTAA T^TAA
437 TTAA
NlaIV 298 GGGCCC GG[ACGT]^[ACGT]CC
741 GGATCC
Sau96I 298 GGGCC G^G[ACGT]CC
299 GGCCC
SfaNI 370 GATGC GCATC(5/9)
694 GATGC
SmlI 44 CTCAAG C^T[CT][GA]AG
499 CTCAAG
TfiI 56 GATTC G^A[AT]TC
341 GAATC
Tru9I 10 TTAA T^TAA
437 TTAA
XhoII 420 AGATCC [GA]^GATC[CT]
741 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BsaJI BsrI BssKI BstF5I
BstNI DdeI DpnII EcoRII Fnu4HI
FokI HinfI ItaI MboI MboII
MvaI NdeII NlaIII RsaI Sau3AI
ScrFI TseI Tsp509I