Restriction map for
pFN21AE6672
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# NO CUT ENZYMES ( 174 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanII BbeI BbrPI BbsI BcgI
BciVI BcnI BglI BglII BlnI
BlpI BmyI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrGI BssHII
BssKI BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstUI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRII EcoT22I FauI FseI FspI
HaeII Hin1I HincII HindII HindIII
HpaI HphI KasI KpnI Ksp632I
KspI MaeII MamI MluI MluNI
MroI MscI MspA1I MvaI MvnI
NarI NciI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI ScrFI SexAI SfaNI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
TaqI TfiI Tth111I TthHB8I Van91I
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AflII 594 CTTAAG C^TTAAG
AlwNI 154 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
BanI 859 GGTGCC G^G[CT][GA]CC
BbvCI 114 GCTGAGG CCTCAGC(-5/-2)
BclI 501 TGATCA T^GATCA
BfrI 594 CTTAAG C^TTAAG
BpmI 164 CTCCAG CTGGAG(16/14)
BsaJI 322 CCTTGG C^C[ACGT][ACGT]GG
BsgI 37 GTGCAG GTGCAG(16/14)
BsmAI 635 GAGAC GTCTC(1/5)
BsmI 402 GAATGC GAATGC(1/-1)
BspMI 897 ACCTGC ACCTGC(4/8)
BsrDI 45 CATTGC GCAATG(2/0)
BsrFI 24 GCCGGC [GA]^CCGG[CT]
BstYI 156 GGATCT [GA]^GATC[CT]
CfoI 42 GCGC GCG^C
Cfr10I 24 GCCGGC [GA]^CCGG[CT]
Cfr13I 281 GGGCC G^G[ACGT]CC
DdeI 115 CTGAG C^T[ACGT]AG
DraI 831 TTTAAA TTT^AAA
EcoRV 607 GATATC GAT^ATC
EcoT14I 322 CCTTGG C^C[AT][AT]GG
FbaI 501 TGATCA T^GATCA
HapII 25 CCGG C^CGG
HgaI 318 GCGTC GACGC(5/10)
HhaI 42 GCGC GCG^C
HinP1I 42 GCGC G^CGC
HpaII 25 CCGG C^CGG
MaeIII 519 GTCAC ^GT[ACGT]AC
MfeI 641 CAATTG C^AATTG
MflI 156 GGATCT [GA]^GATC[CT]
MslI 94 CACCTAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 25 CCGG C^CGG
MunI 641 CAATTG C^AATTG
NaeI 24 GCCGGC GCC^GGC
NgoMIV 24 GCCGGC G^CCGGC
Sau96I 281 GGGCC G^G[ACGT]CC
SphI 879 GCATGC GCATG^C
SspI 694 AATATT AAT^ATT
StuI 351 AGGCCT AGG^CCT
StyI 322 CCTTGG C^C[AT][AT]GG
SwaI 830 ATTTAAAT ATTT^AAAT
Tsp45I 519 GTCAC ^GT[GC]AC
XbaI 477 TCTAGA T^CTAGA
XcmI 203 CCATCTATTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 156 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 12 enzymes) #
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AciI 49 GCGG CCGC(-3/-1)
561 GCGG
AlwI 651 GATCC GGATC(4/5)
156 GGATC
BspHI 303 TCATGA T^CATGA
870 TCATGA
BstF5I 75 GGATG GGATG(2/0)
563 GGATG
EcoNI 96 CCTAGATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
354 CCTTATGAAGG
FokI 75 GGATG GGATG(9/13)
563 GGATG
HinfI 517 GAGTC G^A[ACGT]TC
637 GACTC
MwoI 283 GCCAAAATAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
715 GCTGGACAAGC
NspI 756 ACATGC [GA]CATG^[CT]
879 GCATGC
PleI 637 GACTC GAGTC(4/5)
517 GAGTC
RcaI 303 TCATGA T^CATGA
870 TCATGA
SmlI 16 CTTGAG C^T[CT][GA]AG
594 CTTAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AcsI AluI ApoI BbvI BfaI
BsiYI BslI BsrI Cac8I DpnII
Fnu4HI HaeIII ItaI MaeI MboI
MboII MnlI MseI NdeII NlaIII
NlaIV Sau3AI Tru9I TseI Tsp509I
TspRI