Restriction map for
pFN21AE4572
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# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaLI AscI Asp718I AspEI
AspI AviII AvrII BbrPI BbsI
BfaI BfrI BglI BlnI BpuAI
BsaBI BsaI BseAI BseRI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
Bsu36I Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoT22I FauI FseI FspI
HpaI KpnI KspI MaeI MamI
MfeI MluI MroI MunI NaeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI StuI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccII 139 CGCG CG^CG
AcyI 2143 GGCGCC G[GA]^CG[CT]C
ApaI 1560 GGGCCC GGGCC^C
Asp700I 889 GAATTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1933 CCCGGG C^[CT]CG[GA]G
BbeI 2143 GGCGCC GGCGC^C
BcgI 1003 CGATCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1484 GTATCC GTATCC(6/5)
BclI 630 TGATCA T^GATCA
BglII 1388 AGATCT A^GATCT
BsaHI 2143 GGCGCC G[GA]^CG[CT]C
BsoBI 1933 CCCGGG C^[CT]CG[GA]G
BstUI 139 CGCG CG^CG
BstXI 1720 CCACATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 1782 TTTAAA TTT^AAA
EcoRI 700 GAATTC G^AATTC
FbaI 630 TGATCA T^GATCA
HgaI 739 GACGC GACGC(5/10)
Hin1I 2143 GGCGCC G[GA]^CG[CT]C
HincII 1735 GTTGAC GT[CT]^[GA]AC
HindII 1735 GTTGAC GT[CT]^[GA]AC
HindIII 1921 AAGCTT A^AGCTT
KasI 2143 GGCGCC G^GCGCC
MvnI 139 CGCG CG^CG
NarI 2143 GGCGCC GG^CGCC
NdeI 1811 CATATG CA^TATG
PflMI 214 CCATACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1499 GGGACCT [GA]G^G[AT]CC[CT]
PstI 1504 CTGCAG CTGCA^G
SapI 2127 GCTCTTC GCTCTTC(1/4)
SfoI 2143 GGCGCC GGC^GCC
SmaI 1933 CCCGGG CCC^GGG
Van91I 214 CCATACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2060 CCAAGGACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1933 CCCGGG C^CCGGG
XmaI 1933 CCCGGG C^CCGGG
XmnI 889 GAATTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AflIII 494 ACATGT A^C[GA][CT]GT
1722 ACATGT
AseI 394 ATTAAT AT^TAAT
1318 ATTAAT
AsnI 394 ATTAAT AT^TAAT
1318 ATTAAT
BalI 1894 TGGCCA TGG^CCA
2057 TGGCCA
BlpI 964 GCTGAGC GC^T[ACGT]AGC
1927 GCTGAGC
Bpu1102I 964 GCTGAGC GC^T[ACGT]AGC
1927 GCTGAGC
BsaAI 1468 TACGTG [CT]AC^GT[GA]
1821 TACGTG
BsaWI 334 ACCGGA [AT]^CCGG[AT]
704 TCCGGT
BsgI 88 CTGCAC GTGCAG(16/14)
2138 GTGCAG
BspHI 227 TCATGA T^CATGA
980 TCATGA
BspLU11I 494 ACATGT A^CATGT
1722 ACATGT
BstAPI 1165 GCAAATTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2210 GCAAAGACTGC
BstEII 2034 GGTGACC G^GT[ACGT]ACC
2116 GGTGACC
BstPI 2034 GGTGACC G^GT[ACGT]ACC
2116 GGTGACC
CelII 964 GCTGAGC GC^T[ACGT]AGC
1927 GCTGAGC
DraII 1499 GGGACCT [GA]G^G[ACGT]CC[CT]
1559 AGGGCCC
DraIII 12 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
2101 CACTGTGTG
EarI 7 CTCTTC CTCTTC(1/4)
2128 CTCTTC
EcoO109I 1499 GGGACCT [GA]G^G[ACGT]CC[CT]
1559 AGGGCCC
EcoO65I 2034 GGTGACC G^GT[ACGT]ACC
2116 GGTGACC
EcoRV 406 GATATC GAT^ATC
1306 GATATC
HaeII 1906 AGCGCC [GA]GCGC^[CT]
2143 GGCGCC
Ksp632I 7 CTCTTC CTCTTC(1/4)
2128 CTCTTC
MaeII 1469 ACGT A^CGT
1822 ACGT
MluNI 1894 TGGCCA TGG^CCA
2057 TGGCCA
MscI 1894 TGGCCA TGG^CCA
2057 TGGCCA
MspA1I 362 CAGCTG C[AC]G^C[GT]G
2133 CAGCTG
NcoI 1479 CCATGG C^CATGG
1878 CCATGG
PshBI 394 ATTAAT AT^TAAT
1318 ATTAAT
PvuII 362 CAGCTG CAG^CTG
2133 CAGCTG
RcaI 227 TCATGA T^CATGA
980 TCATGA
SphI 1115 GCATGC GCATG^C
1425 GCATGC
SspI 667 AATATT AAT^ATT
1456 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BamHI
BanI BanII BbvCI BbvI BcnI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EaeI EcoNI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII