##################################
# NO CUT ENZYMES (  175 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BanI        
BbeI        BbrPI       BbsI        BcgI        BciVI       
BclI        BfaI        BfrI        BglI        BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
BsoBI       BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HinfI       
HpaI        KasI        KpnI        KspI        MaeI        
MaeII       MaeIII      MamI        MfeI        MluI        
MroI        MslI        MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        TaqI        TfiI        
Tsp45I      TspRI       Tth111I     TthHB8I     XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AlwNI          76  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
BalI           72  TGGCCA               TGG^CCA
BamHI         179  GGATCC               G^GATCC
BbvCI         419  CCTCAGC              CCTCAGC(-5/-2)
BcnI          372  CCCGG                CC^[GC]GG
BglII         122  AGATCT               A^GATCT
BpmI          241  CTGGAG               CTGGAG(16/14)
BsmAI         214  GAGAC                GTCTC(1/5)
BsmFI         163  GTCCC                GGGAC(10/14)
Bsp1407I      266  TGTACA               T^GTACA
BsrGI         266  TGTACA               T^GTACA
BsrI          391  CCAGT                ACTGG(1/-1)
BstF5I        246  GGATG                GGATG(2/0)
BstXI         167  CCATTTTCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I          74  GCCAGC               GC[ACGT]^[ACGT]GC
DsaI           14  CCATGG               C^C[GA][CT]GG
EaeI           72  TGGCCA               [CT]^GGCC[GA]
EcoNI         136  CCTAACAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FokI          246  GGATG                GGATG(9/13)
HapII         373  CCGG                 C^CGG
HpaII         373  CCGG                 C^CGG
MluNI          72  TGGCCA               TGG^CCA
MscI           72  TGGCCA               TGG^CCA
MseI           35  TTAA                 T^TAA
MspI          373  CCGG                 C^CGG
NciI          372  CCCGG                CC^[GC]GG
NcoI           14  CCATGG               C^CATGG
NspI          205  GCATGT               [GA]CATG^[CT]
PflMI          75  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         161  AGGTCCC              [GA]G^G[AT]CC[CT]
SfcI          235  CTACAG               C^T[GA][CT]AG
SmlI          334  CTTGAG               C^T[CT][GA]AG
SspBI         266  TGTACA               T^GTACA
Tru9I          35  TTAA                 T^TAA
Tsp509I        33  AATT                 ^AATT
Van91I         75  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AvaII         150  GGACC                G^G[AT]CC
              162  GGTCC               
BanII         379  GAGCCC               G[GA]GC[CT]^C
              415  GAGCCC              
BmyI          379  GAGCCC               G[AGT]GC[ACT]^C
              415  GAGCCC              
BsgI          126  CTGCAC               GTGCAG(16/14)
              113  GTGCAG              
Bsp1286I      379  GAGCCC               G[AGT]GC[ACT]^C
              415  GAGCCC              
DdeI          315  CTCAG                C^T[ACGT]AG
              420  CTCAG               
DraII         161  AGGTCCC              [GA]G^G[ACGT]CC[CT]
              225  GGGGCCT             
EarI          220  GAAGAG               CTCTTC(1/4)
              280  GAAGAG              
EcoO109I      161  AGGTCCC              [GA]G^G[ACGT]CC[CT]
              225  GGGGCCT             
Ksp632I       220  GAAGAG               CTCTTC(1/4)
              280  GAAGAG              
NlaIII         15  CATG                 CATG^
              206  CATG                
PvuII          76  CAGCTG               CAG^CTG
              238  CAGCTG              


#########################################
# 3 OR MORE CUT ENZYMES (   35 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BbvI        
BsaJI       BsiYI       BslI        BssKI       BstNI       
BstYI       Cfr13I      DpnII       EcoRII      EcoT14I     
Fnu4HI      HaeIII      HphI        ItaI        MboI        
MboII       MflI        MnlI        MspA1I      MvaI        
MwoI        NdeII       NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       StyI        TseI        XhoII