Restriction map for
pFN21AE1997
##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI Asp718I AspEI AvaI AviII
AvrII BciVI BfrI BglII BlnI
BsiEI BsiWI BsmBI BsoBI BspDI
BspLU11I BsrBI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FseI FspI
HincII HindII HpaI KpnI Ksp632I
KspI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmeI
PpuMI Psp1406I PvuI RsrII SacI
SacII SalI SapI SexAI SfuI
SmaI SmlI SpeI SphI SspI
StuI SwaI Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 2274 GACGTC GACGT^C
AccI 1140 GTCTAC GT^[AC][GT]AC
AccII 114 CGCG CG^CG
AccIII 1256 TCCGGA T^CCGGA
ApaI 79 GGGCCC GGGCC^C
AseI 1246 ATTAAT AT^TAAT
AsnI 1246 ATTAAT AT^TAAT
Asp700I 976 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 2110 GTGCTC G[AT]GC[AT]^C
AspI 1862 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 278 TGGCCA TGG^CCA
BamHI 260 GGATCC G^GATCC
BbrPI 229 CACGTG CAC^GTG
BbvCI 594 CCTCAGC CCTCAGC(-5/-2)
BcgI 47 CGATGCTGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1521 TGATCA T^GATCA
BlpI 1095 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1095 GCTGAGC GC^T[ACGT]AGC
BsaWI 1256 TCCGGA [AT]^CCGG[AT]
BseAI 1256 TCCGGA T^CCGGA
BsgI 232 GTGCAG GTGCAG(16/14)
BsiHKAI 2110 GTGCTC G[AT]GC[AT]^C
BsmI 586 GCATTC GAATGC(1/-1)
BspEI 1256 TCCGGA T^CCGGA
BspHI 1085 TCATGA T^CATGA
BssSI 1159 CACGAG CACGAG(-5/-1)
BstAPI 1003 GCAATCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 114 CGCG CG^CG
CelII 1095 GCTGAGC GC^T[ACGT]AGC
DraII 78 GGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 1556 GACATTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1233 CCTGACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 78 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 537 GAATTC G^AATTC
FbaI 1521 TGATCA T^GATCA
MluNI 278 TGGCCA TGG^CCA
MroI 1256 TCCGGA T^CCGGA
MscI 278 TGGCCA TGG^CCA
MvnI 114 CGCG CG^CG
NcoI 125 CCATGG C^CATGG
NspI 1686 ACATGC [GA]CATG^[CT]
PflFI 1862 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 229 CACGTG CAC^GTG
PmlI 229 CACGTG CAC^GTG
PshAI 1236 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1246 ATTAAT AT^TAAT
PstI 67 CTGCAG CTGCA^G
RcaI 1085 TCATGA T^CATGA
SbfI 66 CCTGCAGG CCTGCA^GG
SfiI 270 GGCCTCCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1915 CGCCGGCG C[GA]^CCGG[CT]G
SnaBI 1036 TACGTA TAC^GTA
Sse8387I 66 CCTGCAGG CCTGCA^GG
Tth111I 1862 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 976 GAAATGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 537 GAATTC [GA]^AATT[CT]
1799 AAATTC
AlwNI 616 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
1703 CAGAGACTG
ApoI 537 GAATTC [GA]^AATT[CT]
1799 AAATTC
BanII 79 GGGCCC G[GA]GC[CT]^C
1214 GAGCCC
BbeI 33 GGCGCC GGCGC^C
88 GGCGCC
BcnI 172 CCCGG CC^[GC]GG
2099 CCGGG
BfaI 149 CTAG C^TAG
361 CTAG
BglI 271 GCCTCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2367 GCCACCACGGC
BsaAI 229 CACGTG [CT]AC^GT[GA]
1036 TACGTA
BsaI 100 GAGACC GGTCTC(1/5)
799 GAGACC
Bsp1407I 758 TGTACA T^GTACA
947 TGTACA
BspMI 620 ACCTGC ACCTGC(4/8)
69 GCAGGT
BsrGI 758 TGTACA T^GTACA
947 TGTACA
BstXI 443 CCATCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1691 CCATTGAGATGG
BstYI 236 AGATCC [GA]^GATC[CT]
260 GGATCC
EaeI 278 TGGCCA [CT]^GGCC[GA]
2217 TGGCCG
EcoT14I 125 CCATGG C^C[AT][AT]GG
1898 CCAAGG
HgaI 180 GACGC GACGC(5/10)
2089 GACGC
KasI 33 GGCGCC G^GCGCC
88 GGCGCC
MaeI 149 CTAG C^TAG
361 CTAG
MflI 236 AGATCC [GA]^GATC[CT]
260 GGATCC
MspA1I 644 CAGCTG C[AC]G^C[GT]G
971 CAGCTG
NarI 33 GGCGCC GG^CGCC
88 GGCGCC
NciI 172 CCCGG CC^[GC]GG
2099 CCGGG
PvuII 644 CAGCTG CAG^CTG
971 CAGCTG
ScaI 1079 AGTACT AGT^ACT
1720 AGTACT
SfcI 67 CTGCAG C^T[GA][CT]AG
2287 CTACAG
SfoI 33 GGCGCC GGC^GCC
88 GGCGCC
SspBI 758 TGTACA T^GTACA
947 TGTACA
StyI 125 CCATGG C^C[AT][AT]GG
1898 CCAAGG
Tsp45I 1565 GTCAC ^GT[GC]AC
1777 GTCAC
XcmI 7 CCACTGGGCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2371 CCACGGCTGGTCTGG
XhoII 236 AGATCC [GA]^GATC[CT]
260 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AvaII BanI BbsI BbvI BmyI
BpmI BpuAI BsaBI BsaHI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrFI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DsaI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI MaeII
MaeIII MamI MboI MboII MnlI
MseI MslI MspI MvaI MwoI
NaeI NdeII NgoMIV NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I