Restriction map for
pFN21AE1973
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BanI BanII BbeI BbrPI
BbvCI BbvI BcgI BciVI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BseAI BsgI BsiEI BsiWI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI FauI Fnu4HI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI ItaI KasI KpnI KspI
MamI MfeI MluI MroI MunI
MvnI NaeI NarI NciI NcoI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI Sse8387I SspBI StuI SwaI
TseI Tth111I XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AflIII 192 ACATGT A^C[GA][CT]GT
AspHI 1174 GAGCAC G[AT]GC[AT]^C
AvaI 219 CTCGAG C^[CT]CG[GA]G
AvaII 655 GGACC G^G[AT]CC
BamHI 771 GGATCC G^GATCC
BclI 992 TGATCA T^GATCA
BmyI 1174 GAGCAC G[AGT]GC[ACT]^C
BsiHKAI 1174 GAGCAC G[AT]GC[AT]^C
BsmAI 1586 GAGAC GTCTC(1/5)
BsmBI 1586 GAGACG CGTCTC(1/5)
BsmFI 74 GTCCC GGGAC(10/14)
BsmI 1150 GAATGC GAATGC(1/-1)
BsoBI 219 CTCGAG C^[CT]CG[GA]G
Bsp1286I 1174 GAGCAC G[AGT]GC[ACT]^C
BspLU11I 192 ACATGT A^CATGT
BsrDI 118 CATTGC GCAATG(2/0)
BssSI 123 CTCGTG CACGAG(-5/-1)
BstXI 1202 CCAGGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 655 GGACC G^G[ACGT]CC
EcoRV 961 GATATC GAT^ATC
EcoT14I 208 CCAAGG C^C[AT][AT]GG
EcoT22I 1236 ATGCAT ATGCA^T
FbaI 992 TGATCA T^GATCA
MspA1I 351 CAGCTG C[AC]G^C[GT]G
NdeI 1121 CATATG CA^TATG
NsiI 1236 ATGCAT ATGCA^T
PaeR7I 219 CTCGAG C^TCGAG
PflMI 158 CCAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 53 CTGCAG CTGCA^G
PvuII 351 CAGCTG CAG^CTG
Sau96I 655 GGACC G^G[ACGT]CC
SfaNI 1126 GATGC GCATC(5/9)
SfcI 53 CTGCAG C^T[GA][CT]AG
SpeI 376 ACTAGT A^CTAGT
SphI 828 GCATGC GCATG^C
SspI 856 AATATT AAT^ATT
StyI 208 CCAAGG C^C[AT][AT]GG
TaqI 220 TCGA T^CGA
TfiI 58 GAATC G^A[AT]TC
Tsp45I 1033 GTGAC ^GT[GC]AC
TthHB8I 220 TCGA T^CGA
Van91I 158 CCAAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 814 CCAGAAGGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 219 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 400 AAATTT [GA]^AATT[CT]
1423 GAATTT
AfaI 65 GTAC GT^AC
168 GTAC
ApoI 400 AAATTT [GA]^AATT[CT]
1423 GAATTT
BalI 256 TGGCCA TGG^CCA
899 TGGCCA
BbsI 1310 GAAGAC GAAGAC(2/6)
976 GTCTTC
BfaI 377 CTAG C^TAG
1030 CTAG
BpuAI 1310 GAAGAC GAAGAC(2/6)
976 GTCTTC
BsaWI 642 TCCGGT [AT]^CCGG[AT]
1593 ACCGGA
BseRI 1171 GAGGAG GAGGAG(10/8)
1265 GAGGAG
BsiYI 158 CCAAAAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1159 CCTGAAAATGG
BslI 158 CCAAAAGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1159 CCTGAAAATGG
BstYI 280 GGATCT [GA]^GATC[CT]
771 GGATCC
DdeI 311 CTCAG C^T[ACGT]AG
1327 CTAAG
EaeI 256 TGGCCA [CT]^GGCC[GA]
899 TGGCCA
HapII 643 CCGG C^CGG
1594 CCGG
HincII 445 GTCAAC GT[CT]^[GA]AC
1057 GTTGAC
HindII 445 GTCAAC GT[CT]^[GA]AC
1057 GTTGAC
HpaII 643 CCGG C^CGG
1594 CCGG
MaeI 377 CTAG C^TAG
1030 CTAG
MflI 280 GGATCT [GA]^GATC[CT]
771 GGATCC
MluNI 256 TGGCCA TGG^CCA
899 TGGCCA
MscI 256 TGGCCA TGG^CCA
899 TGGCCA
MspI 643 CCGG C^CGG
1594 CCGG
MwoI 905 GCACGGGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
949 GCAGACATGGC
NlaIV 99 GGAACC GG[ACGT]^[ACGT]CC
771 GGATCC
NspI 192 ACATGT [GA]CATG^[CT]
828 GCATGC
RsaI 65 GTAC GT^AC
168 GTAC
SmlI 219 CTCGAG C^T[CT][GA]AG
1527 CTCAAG
XhoII 280 GGATCT [GA]^GATC[CT]
771 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AluI AlwI BpmI BsaJI BsrI
BssKI BstF5I BstNI Cac8I DpnII
EarI EcoRII FokI HaeIII HindIII
HinfI HphI Ksp632I MaeII MaeIII
MboI MboII MnlI MseI MslI
MvaI NdeII NlaIII PleI Sau3AI
ScrFI Tru9I Tsp509I TspRI