Restriction map for
pFN21AE5752
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AvrII BamHI BbrPI BciVI
BclI BfaI BlnI BlpI Bpu1102I
BsaAI BseAI BsgI BsiWI BspEI
BspHI BspLU11I BsrBI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I CelII
CpoI DraI DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI Ksp632I
KspI MaeI MfeI MluI MroI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I RcaI RsrII SacII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI XbaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 856 GAATTC [GA]^AATT[CT]
AcyI 1037 GGCGCC G[GA]^CG[CT]C
AflII 319 CTTAAG C^TTAAG
ApoI 856 GAATTC [GA]^AATT[CT]
AspI 123 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 239 TGCGCA TGC^GCA
BanI 1037 GGCGCC G^G[CT][GA]CC
BbeI 1037 GGCGCC GGCGC^C
BcgI 1631 GCAGCTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 319 CTTAAG C^TTAAG
BglII 69 AGATCT A^GATCT
BsaHI 1037 GGCGCC G[GA]^CG[CT]C
BsiEI 473 CGATCG CG[GA][CT]^CG
BspDI 1834 ATCGAT AT^CGAT
BspMI 242 GCAGGT ACCTGC(4/8)
BsrFI 196 ACCGGC [GA]^CCGG[CT]
BstAPI 1263 GCAGTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 196 ACCGGC [GA]^CCGG[CT]
ClaI 1834 ATCGAT AT^CGAT
DrdI 997 GACTCCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1734 CCTCTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 856 GAATTC G^AATTC
FspI 239 TGCGCA TGC^GCA
Hin1I 1037 GGCGCC G[GA]^CG[CT]C
KasI 1037 GGCGCC G^GCGCC
MaeII 1412 ACGT A^CGT
NarI 1037 GGCGCC GG^CGCC
NcoI 152 CCATGG C^CATGG
PaeR7I 679 CTCGAG C^TCGAG
PflFI 123 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 473 CGATCG CGAT^CG
PvuII 1894 CAGCTG CAG^CTG
SacI 662 GAGCTC GAGCT^C
SexAI 1924 ACCAGGT A^CC[AT]GGT
SfoI 1037 GGCGCC GGC^GCC
SmaI 2047 CCCGGG CCC^GGG
TfiI 1252 GATTC G^A[AT]TC
Tsp509I 857 AATT ^AATT
Tth111I 123 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 679 CTCGAG C^TCGAG
XmaCI 2047 CCCGGG C^CCGGG
XmaI 2047 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 1006 GTCTAC GT^[AC][GT]AC
1147 GTAGAC
Alw44I 262 GTGCAC G^TGCAC
1414 GTGCAC
AlwNI 564 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1810 CAGATCCTG
ApaLI 262 GTGCAC G^TGCAC
1414 GTGCAC
AvaI 679 CTCGAG C^[CT]CG[GA]G
2047 CCCGGG
BbvCI 93 CCTCAGC CCTCAGC(-5/-2)
1620 CCTCAGC
BsaI 157 GAGACC GGTCTC(1/5)
1102 GAGACC
BsaWI 282 ACCGGA [AT]^CCGG[AT]
1313 TCCGGT
BsmBI 1981 CGTCTC CGTCTC(1/5)
1300 GAGACG
BsoBI 679 CTCGAG C^[CT]CG[GA]G
2047 CCCGGG
Bsp1407I 923 TGTACA T^GTACA
1556 TGTACA
BsrDI 344 GCAATG GCAATG(2/0)
1190 GCAATG
BsrGI 923 TGTACA T^GTACA
1556 TGTACA
BstEII 1284 GGTCACC G^GT[ACGT]ACC
1602 GGTCACC
BstPI 1284 GGTCACC G^GT[ACGT]ACC
1602 GGTCACC
BstYI 69 AGATCT [GA]^GATC[CT]
1811 AGATCC
EcoO65I 1284 GGTCACC G^GT[ACGT]ACC
1602 GGTCACC
FauI 1305 GCGGG CCCGC(4/6)
1855 GCGGG
HaeII 394 AGCGCC [GA]GCGC^[CT]
1037 GGCGCC
HgaI 1302 GACGC GACGC(5/10)
1980 GCGTC
MaeIII 1285 GTCAC ^GT[ACGT]AC
1603 GTCAC
MflI 69 AGATCT [GA]^GATC[CT]
1811 AGATCC
MspA1I 507 CCGCTG C[AC]G^C[GT]G
1894 CAGCTG
NspI 258 GCATGT [GA]CATG^[CT]
1268 ACATGC
PflMI 1027 CCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1608 CCAGTTCGTGG
SspBI 923 TGTACA T^GTACA
1556 TGTACA
Tsp45I 1285 GTCAC ^GT[GC]AC
1603 GTCAC
Van91I 1027 CCAGAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1608 CCAGTTCGTGG
XcmI 1781 CCAAGAAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2001 CCAGGTGAAGGGTGG
XhoII 69 AGATCT [GA]^GATC[CT]
1811 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BalI BanII BbsI
BbvI BcnI BglI BmyI BpmI
BpuAI BsaBI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MamI MboI
MboII MluNI MnlI MscI MseI
MslI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI Tru9I TseI TspRI TthHB8I