Restriction map for
pFN21AE6573
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI AocI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
Cfr10I CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI HincII HindII HindIII HinfI
HpaI KpnI Ksp632I KspI MamI
MfeI MflI MluI MluNI MroI
MscI MslI MunI MvnI NaeI
NcoI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspI StuI SwaI TfiI Tsp45I
Tsp509I TspRI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AcyI 487 GGCGCC G[GA]^CG[CT]C
Alw44I 355 GTGCAC G^TGCAC
AlwI 11 GGATC GGATC(4/5)
AlwNI 392 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 580 AGCGCT AGC^GCT
ApaI 504 GGGCCC GGGCC^C
ApaLI 355 GTGCAC G^TGCAC
AspHI 355 GTGCAC G[AT]GC[AT]^C
AvaI 501 CTCGGG C^[CT]CG[GA]G
BanII 504 GGGCCC G[GA]GC[CT]^C
BbeI 487 GGCGCC GGCGC^C
BcnI 517 CCGGG CC^[GC]GG
BfaI 584 CTAG C^TAG
BglI 388 GCCACAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 487 GGCGCC G[GA]^CG[CT]C
BsiHKAI 355 GTGCAC G[AT]GC[AT]^C
BsmBI 482 GAGACG CGTCTC(1/5)
BsmFI 417 GGGAC GGGAC(10/14)
BsoBI 501 CTCGGG C^[CT]CG[GA]G
BspDI 307 ATCGAT AT^CGAT
BsrI 122 ACTGG ACTGG(1/-1)
BstXI 20 CCAGGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 307 ATCGAT AT^CGAT
DpnII 12 GATC ^GATC
Eco47III 580 AGCGCT AGC^GCT
EcoT14I 413 CCAAGG C^C[AT][AT]GG
HapII 517 CCGG C^CGG
Hin1I 487 GGCGCC G[GA]^CG[CT]C
HpaII 517 CCGG C^CGG
KasI 487 GGCGCC G^GCGCC
MaeI 584 CTAG C^TAG
MaeII 380 ACGT A^CGT
MaeIII 341 GTTAC ^GT[ACGT]AC
MboI 12 GATC ^GATC
MseI 95 TTAA T^TAA
MspI 517 CCGG C^CGG
NarI 487 GGCGCC GG^CGCC
NciI 517 CCGGG CC^[GC]GG
NdeII 12 GATC ^GATC
NheI 583 GCTAGC G^CTAGC
NspI 455 GCATGC [GA]CATG^[CT]
PpuMI 461 AGGACCT [GA]G^G[AT]CC[CT]
Sau3AI 12 GATC ^GATC
SfaNI 346 GATGC GCATC(5/9)
SfoI 487 GGCGCC GGC^GCC
SphI 455 GCATGC GCATG^C
StyI 413 CCAAGG C^C[AT][AT]GG
TaqI 308 TCGA T^CGA
Tru9I 95 TTAA T^TAA
TthHB8I 308 TCGA T^CGA
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# TWO CUT ENZYMES ( 22 enzymes) #
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AluI 448 AGCT AG^CT
589 AGCT
AvaII 321 GGTCC G^G[AT]CC
462 GGACC
BmyI 355 GTGCAC G[AGT]GC[ACT]^C
504 GGGCCC
BpmI 18 CTCCAG CTGGAG(16/14)
249 CTCCAG
Bsp1286I 355 GTGCAC G[AGT]GC[ACT]^C
504 GGGCCC
Bsp1407I 260 TGTACA T^GTACA
273 TGTACA
BsrGI 260 TGTACA T^GTACA
273 TGTACA
BstF5I 277 CATCC GGATG(2/0)
113 GGATG
CfoI 488 GCGC GCG^C
581 GCGC
DraII 461 AGGACCT [GA]G^G[ACGT]CC[CT]
504 GGGCCCC
EaeI 383 TGGCCG [CT]^GGCC[GA]
546 CGGCCA
EcoO109I 461 AGGACCT [GA]G^G[ACGT]CC[CT]
504 GGGCCCC
FauI 369 GCGGG CCCGC(4/6)
528 GCGGG
FokI 277 CATCC GGATG(9/13)
113 GGATG
HaeII 487 GGCGCC [GA]GCGC^[CT]
580 AGCGCT
HhaI 488 GCGC GCG^C
581 GCGC
HinP1I 488 GCGC G^CGC
581 GCGC
HphI 143 TCACC GGTGA(8/7)
467 TCACC
MboII 170 TCTTC GAAGA(8/7)
289 TCTTC
MspA1I 392 CAGCGG C[AC]G^C[GT]G
534 CAGCGG
NlaIII 8 CATG CATG^
456 CATG
SspBI 260 TGTACA T^GTACA
273 TGTACA
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
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AciI AfaI BanI BbvI BsaJI
BsiYI BslI BsmAI BssKI BstNI
Cac8I Cfr13I DdeI EcoRII Fnu4HI
HaeIII ItaI MnlI MvaI MwoI
NlaIV RsaI Sau96I ScrFI TseI