Restriction map for
pFN21AE4363
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# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AocI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AviII BbeI
BbrPI BbsI BbvCI BcgI BfrI
BglII BpuAI BsaAI BsaBI BsaHI
BsaI BseAI BseRI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HgaI Hin1I HincII HindII HindIII
HpaI HphI KasI Ksp632I KspI
MamI MboII MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfoI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI SwaI XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 711 GGTACC G^GTACC
AfaI 712 GTAC GT^AC
AflIII 183 ACATGT A^C[GA][CT]GT
Asp718I 711 GGTACC G^GTACC
AspHI 828 GTGCTC G[AT]GC[AT]^C
AvrII 153 CCTAGG C^CTAGG
BalI 245 TGGCCA TGG^CCA
BamHI 650 GGATCC G^GATCC
BciVI 1124 GTATCC GTATCC(6/5)
BclI 641 TGATCA T^GATCA
BlnI 153 CCTAGG C^CTAGG
BlpI 748 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 748 GCTGAGC GC^T[ACGT]AGC
BsaWI 824 TCCGGT [AT]^CCGG[AT]
BsgI 144 GTGCAG GTGCAG(16/14)
BsiHKAI 828 GTGCTC G[AT]GC[AT]^C
BsmAI 884 GAGAC GTCTC(1/5)
BspLU11I 183 ACATGT A^CATGT
CelII 748 GCTGAGC GC^T[ACGT]AGC
DrdI 520 GACAAGCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 833 CCATGG C^C[GA][CT]GG
EaeI 245 TGGCCA [CT]^GGCC[GA]
EcoNI 285 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 641 TGATCA T^GATCA
KpnI 711 GGTACC GGTAC^C
MaeII 302 ACGT A^CGT
MluNI 245 TGGCCA TGG^CCA
MscI 245 TGGCCA TGG^CCA
MseI 236 TTAA T^TAA
NcoI 833 CCATGG C^CATGG
NheI 678 GCTAGC G^CTAGC
PpuMI 494 GGGACCC [GA]G^G[AT]CC[CT]
RsaI 712 GTAC GT^AC
SfiI 246 GGCCAAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 544 CCCGGG CCC^GGG
SphI 791 GCATGC GCATG^C
TfiI 663 GATTC G^A[AT]TC
Tru9I 236 TTAA T^TAA
XmaCI 544 CCCGGG C^CCGGG
XmaI 544 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 706 CGCG CG^CG
881 CGCG
Aor51HI 752 AGCGCT AGC^GCT
844 AGCGCT
ApaI 102 GGGCCC GGGCC^C
548 GGGCCC
AspI 220 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1101 GACCATGTC
AvaI 544 CCCGGG C^[CT]CG[GA]G
1087 CTCGGG
BfaI 154 CTAG C^TAG
679 CTAG
BglI 247 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
550 GCCCTCCAGGC
BsoBI 544 CCCGGG C^[CT]CG[GA]G
1087 CTCGGG
BspMI 14 ACCTGC ACCTGC(4/8)
1075 ACCTGC
BstAPI 21 GCAGTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
132 GCAAGCATTGC
BstUI 706 CGCG CG^CG
881 CGCG
BstYI 582 GGATCT [GA]^GATC[CT]
650 GGATCC
Eco47III 752 AGCGCT AGC^GCT
844 AGCGCT
FauI 492 GCGGG CCCGC(4/6)
724 GCGGG
HaeII 752 AGCGCT [GA]GCGC^[CT]
844 AGCGCT
MaeI 154 CTAG C^TAG
679 CTAG
MaeIII 9 GTCAC ^GT[ACGT]AC
913 GTGAC
MflI 582 GGATCT [GA]^GATC[CT]
650 GGATCC
MspA1I 802 CAGCTG C[AC]G^C[GT]G
1033 CAGCTG
MvnI 706 CGCG CG^CG
881 CGCG
PflFI 220 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1101 GACCATGTC
PstI 19 CTGCAG CTGCA^G
630 CTGCAG
PvuII 802 CAGCTG CAG^CTG
1033 CAGCTG
StuI 562 AGGCCT AGG^CCT
968 AGGCCT
TaqI 371 TCGA T^CGA
773 TCGA
Tsp45I 9 GTCAC ^GT[GC]AC
913 GTGAC
Tsp509I 599 AATT ^AATT
1010 AATT
Tth111I 220 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
1101 GACCATGTC
TthHB8I 371 TCGA T^CGA
773 TCGA
XhoII 582 GGATCT [GA]^GATC[CT]
650 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MboI
MnlI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TseI TspRI
Van91I XcmI