Restriction map for
pFN21AE3975
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# NO CUT ENZYMES ( 144 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AviII AvrII BalI
BamHI BanII BbeI BbrPI BcgI
BciVI BfrI BglI BglII BlnI
BlpI BmyI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsiEI BsiHKAI BsiWI
BsmBI BsmI Bsp1286I BspDI BspHI
BspLU11I BspMI BsrDI BssHII Bst1107I
BstBI BstUI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRV EcoT14I
EcoT22I FseI FspI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MluNI MscI MunI MvnI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccIII 572 TCCGGA T^CCGGA
AcsI 337 GAATTC [GA]^AATT[CT]
AlwI 228 GATCC GGATC(4/5)
AlwNI 241 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 337 GAATTC [GA]^AATT[CT]
AvaI 350 CCCGAG C^[CT]CG[GA]G
BanI 297 GGTGCC G^G[CT][GA]CC
BbsI 152 GTCTTC GAAGAC(2/6)
BclI 648 TGATCA T^GATCA
BfaI 197 CTAG C^TAG
BpuAI 152 GTCTTC GAAGAC(2/6)
BsaWI 572 TCCGGA [AT]^CCGG[AT]
BseAI 572 TCCGGA T^CCGGA
BsgI 616 GTGCAG GTGCAG(16/14)
BsmAI 235 GAGAC GTCTC(1/5)
BsmFI 348 GTCCC GGGAC(10/14)
BsoBI 350 CCCGAG C^[CT]CG[GA]G
Bsp1407I 29 TGTACA T^GTACA
BspEI 572 TCCGGA T^CCGGA
BsrBI 484 GAGCGG CCGCTC(-3/-3)
BsrFI 115 GCCGGC [GA]^CCGG[CT]
BsrGI 29 TGTACA T^GTACA
BssSI 232 CACGAG CACGAG(-5/-1)
BstAPI 200 GCAAGTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 366 GGTCACC G^GT[ACGT]ACC
BstPI 366 GGTCACC G^GT[ACGT]ACC
BstXI 372 CCAGCCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 227 AGATCC [GA]^GATC[CT]
CfoI 113 GCGC GCG^C
Cfr10I 115 GCCGGC [GA]^CCGG[CT]
DraII 578 GGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 144 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 578 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 366 GGTCACC G^GT[ACGT]ACC
EcoRI 337 GAATTC G^AATTC
FbaI 648 TGATCA T^GATCA
HaeIII 409 GGCC GG^CC
HgaI 518 GCGTC GACGC(5/10)
HhaI 113 GCGC GCG^C
HinP1I 113 GCGC G^CGC
HinfI 330 GATTC G^A[ACGT]TC
MaeI 197 CTAG C^TAG
MflI 227 AGATCC [GA]^GATC[CT]
MroI 572 TCCGGA T^CCGGA
MseI 224 TTAA T^TAA
MslI 654 CATGACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 681 CCGCTG C[AC]G^C[GT]G
NaeI 115 GCCGGC GCC^GGC
NgoMIV 115 GCCGGC G^CCGGC
NspI 612 GCATGT [GA]CATG^[CT]
PpuMI 578 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 523 CTGCAG CTGCA^G
SapI 385 GAAGAGC GCTCTTC(1/4)
ScaI 203 AGTACT AGT^ACT
SfcI 523 CTGCAG C^T[GA][CT]AG
SspBI 29 TGTACA T^GTACA
TaqI 66 TCGA T^CGA
TfiI 330 GATTC G^A[AT]TC
Tru9I 224 TTAA T^TAA
Tsp509I 338 AATT ^AATT
TthHB8I 66 TCGA T^CGA
XhoII 227 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AspI 265 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
361 GACCGGGTC
BbvCI 607 CCTCAGC CCTCAGC(-5/-2)
556 GCTGAGG
BcnI 267 CCGGG CC^[GC]GG
363 CCGGG
BpmI 19 CTCCAG CTGGAG(16/14)
481 CTGGAG
BsaJI 351 CCGAGG C^C[ACGT][ACGT]GG
405 CCTCGG
BstF5I 698 CATCC GGATG(2/0)
129 GGATG
BstNI 301 CCTGG CC^[AT]GG
480 CCTGG
EcoRII 301 CCTGG ^CC[AT]GG
480 CCTGG
FauI 42 CCCGC CCCGC(4/6)
680 CCCGC
FokI 698 CATCC GGATG(9/13)
129 GGATG
HphI 368 TCACC GGTGA(8/7)
588 TCACC
MaeIII 367 GTCAC ^GT[ACGT]AC
587 GTCAC
MvaI 301 CCTGG CC^[AT]GG
480 CCTGG
NciI 267 CCGGG CC^[GC]GG
363 CCGGG
PflFI 265 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
361 GACCGGGTC
SfaNI 130 GATGC GCATC(5/9)
597 GATGC
Tsp45I 367 GTCAC ^GT[GC]AC
587 GTCAC
Tth111I 265 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
361 GACCGGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 34 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BseRI BsiYI BslI BsrI BssKI
Cac8I Cfr13I DdeI DpnII EarI
Fnu4HI HapII HpaII ItaI Ksp632I
MboI MboII MnlI MspI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI TseI TspRI