Restriction map for
pFN21AE1217
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI AlwI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BcgI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DpnII
DraI DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HpaI HpaII
HphI KasI KpnI Ksp632I KspI
MaeIII MamI MboI MflI MluI
MroI MseI MslI MspA1I MspI
MvnI MwoI NaeI NarI NciI
NcoI NdeI NdeII NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcsI 222 GAATTC [GA]^AATT[CT]
AflIII 461 ACATGT A^C[GA][CT]GT
Alw44I 289 GTGCAC G^TGCAC
ApaLI 289 GTGCAC G^TGCAC
ApoI 222 GAATTC [GA]^AATT[CT]
AspHI 289 GTGCAC G[AT]GC[AT]^C
BalI 185 TGGCCA TGG^CCA
BbvCI 490 GCTGAGG CCTCAGC(-5/-2)
BmyI 289 GTGCAC G[AGT]GC[ACT]^C
BsaJI 389 CCAAGG C^C[ACGT][ACGT]GG
BsiHKAI 289 GTGCAC G[AT]GC[AT]^C
BsiYI 385 CCTCCCAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 385 CCTCCCAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 581 GAGAC GTCTC(1/5)
Bsp1286I 289 GTGCAC G[AGT]GC[ACT]^C
BspLU11I 461 ACATGT A^CATGT
BsrDI 453 CATTGC GCAATG(2/0)
BstF5I 208 GGATG GGATG(2/0)
DraII 392 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 178 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 185 TGGCCA [CT]^GGCC[GA]
EcoO109I 392 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 222 GAATTC G^AATTC
EcoT14I 389 CCAAGG C^C[AT][AT]GG
EcoT22I 282 ATGCAT ATGCA^T
FokI 208 GGATG GGATG(9/13)
HindIII 355 AAGCTT A^AGCTT
MfeI 53 CAATTG C^AATTG
MluNI 185 TGGCCA TGG^CCA
MscI 185 TGGCCA TGG^CCA
MunI 53 CAATTG C^AATTG
NlaIV 369 GGGACC GG[ACGT]^[ACGT]CC
NsiI 282 ATGCAT ATGCA^T
StyI 389 CCAAGG C^C[AT][AT]GG
TaqI 425 TCGA T^CGA
TfiI 474 GATTC G^A[AT]TC
TthHB8I 425 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AfaI 402 GTAC GT^AC
550 GTAC
AvaII 370 GGACC G^G[AT]CC
379 GGACC
BbsI 34 GAAGAC GAAGAC(2/6)
276 GAAGAC
BbvI 150 GCAGC GCAGC(8/12)
253 GCTGC
BfaI 85 CTAG C^TAG
97 CTAG
BpuAI 34 GAAGAC GAAGAC(2/6)
276 GAAGAC
BseRI 12 CTCCTC GAGGAG(10/8)
493 GAGGAG
BsmFI 212 GGGAC GGGAC(10/14)
369 GGGAC
BsrI 339 ACTGG ACTGG(1/-1)
545 CCAGT
BssKI 218 CCTGG ^CC[ACGT]GG
373 CCAGG
BstNI 218 CCTGG CC^[AT]GG
373 CCAGG
Cac8I 3 GCTTGC GC[ACGT]^[ACGT]GC
508 GCAAGC
EcoRII 218 CCTGG ^CC[AT]GG
373 CCAGG
Fnu4HI 150 GCAGC GC^[ACGT]GC
253 GCTGC
HaeIII 186 GGCC GG^CC
394 GGCC
ItaI 150 GCAGC GC^[ACGT]GC
253 GCTGC
MaeI 85 CTAG C^TAG
97 CTAG
MaeII 116 ACGT A^CGT
532 ACGT
MboII 34 GAAGA GAAGA(8/7)
276 GAAGA
MvaI 218 CCTGG CC^[AT]GG
373 CCAGG
NspI 280 ACATGC [GA]CATG^[CT]
461 ACATGT
RsaI 402 GTAC GT^AC
550 GTAC
ScrFI 218 CCTGG CC^[ACGT]GG
373 CCAGG
SfaNI 334 GATGC GCATC(5/9)
498 GATGC
TseI 150 GCAGC G^C[AT]GC
253 GCTGC
Tsp509I 54 AATT ^AATT
223 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AluI Cfr13I DdeI HincII HindII
HinfI MnlI NlaIII PleI Sau96I
TspRI