Restriction map for
pFN21AE5460
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI AvaI AvaII AviII
AvrII BamHI BanI BbeI BbvCI
BcgI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I Cfr13I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoT22I
FauI FbaI FseI FspI HapII
HgaI Hin1I HindIII HpaI HpaII
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MroI MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflMI PinAI PmeI PpuMI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TfiI Van91I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AfaI 63 GTAC GT^AC
AseI 913 ATTAAT AT^TAAT
AsnI 913 ATTAAT AT^TAAT
BalI 248 TGGCCA TGG^CCA
BanII 261 GGGCTC G[GA]GC[CT]^C
BbrPI 926 CACGTG CAC^GTG
BbsI 220 GAAGAC GAAGAC(2/6)
BciVI 573 GTATCC GTATCC(6/5)
BpmI 29 CTGGAG CTGGAG(16/14)
BpuAI 220 GAAGAC GAAGAC(2/6)
BsaAI 926 CACGTG [CT]AC^GT[GA]
BsaI 904 GGTCTC GGTCTC(1/5)
BsmAI 905 GTCTC GTCTC(1/5)
BsmFI 764 GGGAC GGGAC(10/14)
Bsp1407I 62 TGTACA T^GTACA
BsrGI 62 TGTACA T^GTACA
BstXI 251 CCATTCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 650 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 692 GCGC GCG^C
DsaI 803 CCATGG C^C[GA][CT]GG
EaeI 248 TGGCCA [CT]^GGCC[GA]
EcoRV 124 GATATC GAT^ATC
HaeII 691 GGCGCT [GA]GCGC^[CT]
HaeIII 249 GGCC GG^CC
HhaI 692 GCGC GCG^C
HinP1I 692 GCGC G^CGC
HincII 583 GTCAAC GT[CT]^[GA]AC
HindII 583 GTCAAC GT[CT]^[GA]AC
MluNI 248 TGGCCA TGG^CCA
MscI 248 TGGCCA TGG^CCA
NcoI 803 CCATGG C^CATGG
PmaCI 926 CACGTG CAC^GTG
PmlI 926 CACGTG CAC^GTG
PshBI 913 ATTAAT AT^TAAT
RsaI 63 GTAC GT^AC
SmlI 655 CTCAAG C^T[CT][GA]AG
SspBI 62 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcsI 664 GAATTT [GA]^AATT[CT]
985 GAATTT
ApoI 664 GAATTT [GA]^AATT[CT]
985 GAATTT
Asp700I 318 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
554 GAAACCATTC
AspI 151 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
754 GACCACGTC
BmyI 179 GTGCCC G[AGT]GC[ACT]^C
261 GGGCTC
BsgI 338 GTGCAG GTGCAG(16/14)
679 GTGCAG
Bsp1286I 179 GTGCCC G[AGT]GC[ACT]^C
261 GGGCTC
BsrI 28 ACTGG ACTGG(1/-1)
722 CCAGT
BstYI 79 GGATCT [GA]^GATC[CT]
939 GGATCT
DdeI 345 CTTAG C^T[ACGT]AG
569 CTCAG
HphI 597 GGTGA GGTGA(8/7)
128 TCACC
MflI 79 GGATCT [GA]^GATC[CT]
939 GGATCT
MspA1I 35 CAGCTG C[AC]G^C[GT]G
648 CAGCTG
NlaIV 262 GGCTCC GG[ACGT]^[ACGT]CC
778 GGAACC
PflFI 151 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
754 GACCACGTC
PvuII 35 CAGCTG CAG^CTG
648 CAGCTG
TaqI 565 TCGA T^CGA
711 TCGA
TspRI 56 CACTG CA[GC]TG[ACGT][ACGT]^
723 CAGTG
Tth111I 151 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
754 GACCACGTC
TthHB8I 565 TCGA T^CGA
711 TCGA
XhoII 79 GGATCT [GA]^GATC[CT]
939 GGATCT
XmnI 318 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
554 GAAACCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AluI AlwI BbvI BsaJI BsiYI
BslI BssKI BstF5I BstNI DpnII
EcoRII EcoT14I Fnu4HI FokI HinfI
ItaI MaeII MaeIII MboI MboII
MnlI MseI MslI MvaI MwoI
NdeII NlaIII PleI Sau3AI ScrFI
StyI Tru9I TseI Tsp45I Tsp509I