Restriction map for
pFN21AE9535
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I ApaI
ApaLI AscI Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaWI BseAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII Bst1107I
BstEII BstPI BstYI BstZ17I ClaI
CpoI DraI DrdI Eam1105I EarI
EcoNI EcoO65I EcoRI EcoRV FauI
FbaI FseI FspI HgaI HpaI
KpnI Ksp632I KspI MamI MfeI
MflI MluI MluNI MroI MscI
MunI NaeI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tsp45I Tth111I XcmI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 57 enzymes) #
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AccI 1360 GTCTAC GT^[AC][GT]AC
AccII 76 CGCG CG^CG
AcyI 118 GGCGCC G[GA]^CG[CT]C
AocI 1083 CCTTAGG CC^T[ACGT]AGG
Aor51HI 69 AGCGCT AGC^GCT
Asp700I 426 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1028 GTGCTC G[AT]GC[AT]^C
BbeI 118 GGCGCC GGCGC^C
BbsI 430 GTCTTC GAAGAC(2/6)
BlpI 1911 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1911 GCTGAGC GC^T[ACGT]AGC
BpuAI 430 GTCTTC GAAGAC(2/6)
BsaHI 118 GGCGCC G[GA]^CG[CT]C
BsaI 148 GGTCTC GGTCTC(1/5)
BsgI 1314 GTGCAG GTGCAG(16/14)
BsiEI 143 CGGCCG CG[GA][CT]^CG
BsiHKAI 1028 GTGCTC G[AT]GC[AT]^C
BsmAI 149 GTCTC GTCTC(1/5)
BsmI 577 GCATTC GAATGC(1/-1)
BspMI 1738 ACCTGC ACCTGC(4/8)
BsrDI 2045 CATTGC GCAATG(2/0)
BsrFI 145 GCCGGT [GA]^CCGG[CT]
BssSI 65 CACGAG CACGAG(-5/-1)
BstAPI 702 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1658 TTCGAA TT^CGAA
BstUI 76 CGCG CG^CG
BstXI 1547 CCATGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1083 CCTTAGG CC^T[ACGT]AGG
CelII 1911 GCTGAGC GC^T[ACGT]AGC
Cfr10I 145 GCCGGT [GA]^CCGG[CT]
DraIII 2088 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 143 CGGCCG [CT]^GGCC[GA]
EagI 143 CGGCCG C^GGCCG
EclXI 143 CGGCCG C^GGCCG
Eco47III 69 AGCGCT AGC^GCT
Eco52I 143 CGGCCG C^GGCCG
Eco81I 1083 CCTTAGG CC^T[ACGT]AGG
EcoT22I 575 ATGCAT ATGCA^T
Hin1I 118 GGCGCC G[GA]^CG[CT]C
HincII 816 GTTGAC GT[CT]^[GA]AC
HindII 816 GTTGAC GT[CT]^[GA]AC
HindIII 200 AAGCTT A^AGCTT
KasI 118 GGCGCC G^GCGCC
MvnI 76 CGCG CG^CG
NarI 118 GGCGCC GG^CGCC
NcoI 1547 CCATGG C^CATGG
NsiI 575 ATGCAT ATGCA^T
NspI 1443 ACATGC [GA]CATG^[CT]
NspV 1658 TTCGAA TT^CGAA
PflMI 1289 CCATAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1088 GGGTCCT [GA]G^G[AT]CC[CT]
ScaI 1274 AGTACT AGT^ACT
SfoI 118 GGCGCC GGC^GCC
SfuI 1658 TTCGAA TT^CGAA
Van91I 1289 CCATAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1383 TCTAGA T^CTAGA
XmnI 426 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AseI 1105 ATTAAT AT^TAAT
1671 ATTAAT
AsnI 1105 ATTAAT AT^TAAT
1671 ATTAAT
AvaII 1089 GGTCC G^G[AT]CC
1422 GGACC
BanI 118 GGCGCC G^G[CT][GA]CC
1746 GGCACC
BcnI 133 CCCGG CC^[GC]GG
346 CCCGG
BseRI 289 CTCCTC GAGGAG(10/8)
435 CTCCTC
BsmFI 586 GGGAC GGGAC(10/14)
986 GGGAC
DraII 704 AGGCCCT [GA]G^G[ACGT]CC[CT]
1088 GGGTCCT
EcoO109I 704 AGGCCCT [GA]G^G[ACGT]CC[CT]
1088 GGGTCCT
NciI 133 CCCGG CC^[GC]GG
346 CCCGG
PleI 2079 GACTC GAGTC(4/5)
727 GAGTC
PshBI 1105 ATTAAT AT^TAAT
1671 ATTAAT
PstI 22 CTGCAG CTGCA^G
49 CTGCAG
PvuII 25 CAGCTG CAG^CTG
1772 CAGCTG
SfcI 22 CTGCAG C^T[GA][CT]AG
49 CTGCAG
SmlI 197 CTCAAG C^T[CT][GA]AG
381 CTTGAG
SspI 1108 AATATT AAT^ATT
1872 AATATT
TaqI 156 TCGA T^CGA
1659 TCGA
TthHB8I 156 TCGA T^CGA
1659 TCGA
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# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI BanII BbvI BfaI
BmyI BpmI BsaJI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TfiI Tru9I TseI Tsp509I TspRI