Restriction map for
pFN21AE6255
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcsI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmFI BspEI BspHI
BspMI BsrBI BsrDI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I CelII CfoI CpoI
DraI DraIII DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HhaI HinP1I
HincII HindII HindIII HpaI HphI
KasI KpnI KspI MaeI MaeII
MaeIII MamI MfeI MluI MluNI
MroI MscI MslI MunI MvnI
NarI NciI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcyI 128 GACGCC G[GA]^CG[CT]C
AflIII 483 ACATGT A^C[GA][CT]GT
AlwI 600 GGATC GGATC(4/5)
AspHI 358 GTGCTC G[AT]GC[AT]^C
AvaI 457 CCCGAG C^[CT]CG[GA]G
BanII 254 GAGCCC G[GA]GC[CT]^C
BbvCI 100 GCTGAGG CCTCAGC(-5/-2)
BsaHI 128 GACGCC G[GA]^CG[CT]C
BseRI 421 CTCCTC GAGGAG(10/8)
BsiHKAI 358 GTGCTC G[AT]GC[AT]^C
BsmAI 300 GAGAC GTCTC(1/5)
BsmI 525 GAATGC GAATGC(1/-1)
BsoBI 457 CCCGAG C^[CT]CG[GA]G
Bsp1407I 441 TGTACA T^GTACA
BspDI 88 ATCGAT AT^CGAT
BspLU11I 483 ACATGT A^CATGT
BsrFI 407 GCCGGC [GA]^CCGG[CT]
BsrGI 441 TGTACA T^GTACA
BssSI 133 CTCGTG CACGAG(-5/-1)
BstAPI 615 GCAAAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 453 CATCC GGATG(2/0)
BstYI 600 GGATCT [GA]^GATC[CT]
Cac8I 407 GCCGGC GC[ACGT]^[ACGT]GC
Cfr10I 407 GCCGGC [GA]^CCGG[CT]
ClaI 88 ATCGAT AT^CGAT
DpnII 601 GATC ^GATC
DraII 320 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 322 GACCCAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 409 CGGCCA [CT]^GGCC[GA]
EarI 106 GAAGAG CTCTTC(1/4)
EcoNI 426 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 320 AGGACCC [GA]G^G[ACGT]CC[CT]
FokI 453 CATCC GGATG(9/13)
HapII 408 CCGG C^CGG
HgaI 128 GACGC GACGC(5/10)
Hin1I 128 GACGCC G[GA]^CG[CT]C
HpaII 408 CCGG C^CGG
Ksp632I 106 GAAGAG CTCTTC(1/4)
MboI 601 GATC ^GATC
MflI 600 GGATCT [GA]^GATC[CT]
MspA1I 530 CAGCTG C[AC]G^C[GT]G
MspI 408 CCGG C^CGG
NaeI 407 GCCGGC GCC^GGC
NdeII 601 GATC ^GATC
NgoMIV 407 GCCGGC G^CCGGC
NspI 483 ACATGT [GA]CATG^[CT]
PleI 302 GACTC GAGTC(4/5)
PpuMI 320 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 530 CAGCTG CAG^CTG
Sau3AI 601 GATC ^GATC
SfcI 549 CTACAG C^T[GA][CT]AG
SspBI 441 TGTACA T^GTACA
TfiI 228 GAATC G^A[AT]TC
Tsp509I 471 AATT ^AATT
XhoII 600 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
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AccI 331 GTCTAC GT^[AC][GT]AC
547 GTCTAC
AluI 296 AGCT AG^CT
531 AGCT
AlwNI 337 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
364 CAGGGTCTG
AvaII 321 GGACC G^G[AT]CC
492 GGTCC
BmyI 254 GAGCCC G[AGT]GC[ACT]^C
358 GTGCTC
BpmI 361 CTCCAG CTGGAG(16/14)
533 CTGGAG
Bsp1286I 254 GAGCCC G[AGT]GC[ACT]^C
358 GTGCTC
BsrI 354 ACTGG ACTGG(1/-1)
597 ACTGG
EcoT14I 95 CCAAGG C^C[AT][AT]GG
584 CCTTGG
HinfI 228 GAATC G^A[ACGT]TC
302 GACTC
MseI 35 TTAA T^TAA
113 TTAA
NlaIV 321 GGACCC GG[ACGT]^[ACGT]CC
384 GGAACC
SfaNI 91 GATGC GCATC(5/9)
621 GATGC
StyI 95 CCAAGG C^C[AT][AT]GG
584 CCTTGG
TaqI 17 TCGA T^CGA
89 TCGA
Tru9I 35 TTAA T^TAA
113 TTAA
TspRI 285 CAGTG CA[GC]TG[ACGT][ACGT]^
552 CAGTG
TthHB8I 17 TCGA T^CGA
89 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AfaI BbvI BsaJI BsiYI BslI
BssKI BstNI Cfr13I DdeI EcoRII
Fnu4HI HaeIII ItaI MboII MnlI
MvaI MwoI NlaIII RsaI Sau96I
ScrFI TseI