Restriction map for
pFN21AE3979
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AflIII AgeI AocI Aor51HI ApaI
AseI AsnI Asp718I AspI AviII
AvrII BalI BamHI BbrPI BbvCI
BcgI BclI BfrI BlnI BlpI
Bpu1102I BseAI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EarI
Eco47III Eco81I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HgaI
HincII HindII HpaI KpnI Ksp632I
MfeI MluI MluNI MroI MscI
MunI NdeI NotI NruI NsiI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
PstI PvuI PvuII RsrII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI TfiI Tth111I XbaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AclI 950 AACGTT AA^CGTT
AcyI 49 GGCGCC G[GA]^CG[CT]C
AhdI 352 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1013 GTGCAC G^TGCAC
AlwI 513 GATCC GGATC(4/5)
ApaLI 1013 GTGCAC G^TGCAC
AscI 417 GGCGCGCC GG^CGCGCC
Asp700I 81 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 352 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 49 GGCGCC G^G[CT][GA]CC
BbeI 49 GGCGCC GGCGC^C
BciVI 1137 GTATCC GTATCC(6/5)
BglII 1264 AGATCT A^GATCT
BsaAI 226 TACGTG [CT]AC^GT[GA]
BsaBI 1132 GATATGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 49 GGCGCC G[GA]^CG[CT]C
BsaI 753 GAGACC GGTCTC(1/5)
BsaWI 623 ACCGGA [AT]^CCGG[AT]
BsmFI 66 GTCCC GGGAC(10/14)
BsmI 1076 GCATTC GAATGC(1/-1)
BspHI 1248 TCATGA T^CATGA
BspMI 629 GCAGGT ACCTGC(4/8)
BsrBI 123 CCGCTC CCGCTC(-3/-3)
BstXI 843 CCAGAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 302 GGGGCCT [GA]G^G[ACGT]CC[CT]
EagI 383 CGGCCG C^GGCCG
Eam1105I 352 GACCGCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 383 CGGCCG C^GGCCG
Eco52I 383 CGGCCG C^GGCCG
EcoO109I 302 GGGGCCT [GA]G^G[ACGT]CC[CT]
Hin1I 49 GGCGCC G[GA]^CG[CT]C
HindIII 901 AAGCTT A^AGCTT
KasI 49 GGCGCC G^GCGCC
KspI 259 CCGCGG CCGC^GG
MamI 1132 GATATGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 899 TTAA T^TAA
NarI 49 GGCGCC GG^CGCC
NheI 520 GCTAGC G^CTAGC
PflMI 449 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 950 AACGTT AA^CGTT
RcaI 1248 TCATGA T^CATGA
SacII 259 CCGCGG CCGC^GG
SfaNI 707 GCATC GCATC(5/9)
SfcI 1115 CTACAG C^T[GA][CT]AG
SfoI 49 GGCGCC GGC^GCC
SmaI 68 CCCGGG CCC^GGG
SphI 467 GCATGC GCATG^C
StuI 203 AGGCCT AGG^CCT
Tru9I 899 TTAA T^TAA
Van91I 449 CCAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 68 CCCGGG C^CCGGG
XmaI 68 CCCGGG C^CCGGG
XmnI 81 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcsI 1160 GAATTC [GA]^AATT[CT]
1297 GAATTC
ApoI 1160 GAATTC [GA]^AATT[CT]
1297 GAATTC
AvaII 476 GGACC G^G[AT]CC
1044 GGACC
BbsI 923 GTCTTC GAAGAC(2/6)
982 GTCTTC
BfaI 521 CTAG C^TAG
1271 CTAG
BpuAI 923 GTCTTC GAAGAC(2/6)
982 GTCTTC
BseRI 484 GAGGAG GAGGAG(10/8)
1169 GAGGAG
BsgI 112 CTGCAC GTGCAG(16/14)
1237 GTGCAG
BsmAI 753 GAGAC GTCTC(1/5)
724 GTCTC
BssHII 314 GCGCGC G^CGCGC
418 GCGCGC
BstYI 512 AGATCC [GA]^GATC[CT]
1264 AGATCT
DdeI 871 CTTAG C^T[ACGT]AG
1022 CTCAG
EcoRI 1160 GAATTC G^AATTC
1297 GAATTC
EcoT14I 551 CCATGG C^C[AT][AT]GG
998 CCATGG
HinfI 854 GAGTC G^A[ACGT]TC
942 GACTC
MaeI 521 CTAG C^TAG
1271 CTAG
MflI 512 AGATCC [GA]^GATC[CT]
1264 AGATCT
MslI 552 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
798 CATCTCTGTG
NaeI 107 GCCGGC GCC^GGC
640 GCCGGC
NcoI 551 CCATGG C^CATGG
998 CCATGG
NgoMIV 107 GCCGGC G^CCGGC
640 GCCGGC
NspI 356 GCATGT [GA]CATG^[CT]
467 GCATGC
PaeR7I 142 CTCGAG C^TCGAG
322 CTCGAG
PleI 942 GACTC GAGTC(4/5)
854 GAGTC
SacI 271 GAGCTC GAGCT^C
1252 GAGCTC
ScaI 179 AGTACT AGT^ACT
326 AGTACT
StyI 551 CCATGG C^C[AT][AT]GG
998 CCATGG
Tsp45I 856 GTCAC ^GT[GC]AC
911 GTCAC
XcmI 449 CCAGGCGCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
491 CCACGTCGTCGCTGG
XhoI 142 CTCGAG C^TCGAG
322 CTCGAG
XhoII 512 AGATCC [GA]^GATC[CT]
1264 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaI BanII BbvI BcnI
BglI BmyI BpmI BsaJI BsiEI
BsiHKAI BsiYI BslI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DpnII DsaI EaeI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SmlI TaqI
TseI Tsp509I TspRI TthHB8I