Restriction map for
pFN21AE1948
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII AccI AccIII AclI AflII
AflIII AgeI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
AspHI AvaI AviII AvrII BamHI
BanII BbrPI BbsI BbvCI BciVI
BclI BfrI BglI BlnI BlpI
BmyI Bpu1102I BpuAI BsaAI BsaBI
BsaI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HindIII HpaI
KspI MaeII MamI MluI MroI
NaeI NcoI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 1425 GGTACC G^GTACC
AhdI 1399 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 34 CCTGAGG CC^T[ACGT]AGG
Asp718I 1425 GGTACC G^GTACC
AspEI 1399 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1356 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 26 GGTCC G^G[AT]CC
BalI 958 TGGCCA TGG^CCA
BcgI 49 CGATTCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 29 CCGGG CC^[GC]GG
BglII 749 AGATCT A^GATCT
BspMI 123 GCAGGT ACCTGC(4/8)
BstBI 1372 TTCGAA TT^CGAA
BstXI 304 CCAGGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 34 CCTGAGG CC^T[ACGT]AGG
Eam1105I 1399 GACAGAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 34 CCTGAGG CC^T[ACGT]AGG
HapII 29 CCGG C^CGG
HgaI 1360 GCGTC GACGC(5/10)
HincII 1362 GTCAAC GT[CT]^[GA]AC
HindII 1362 GTCAAC GT[CT]^[GA]AC
HpaII 29 CCGG C^CGG
KpnI 1425 GGTACC GGTAC^C
MfeI 587 CAATTG C^AATTG
MluNI 958 TGGCCA TGG^CCA
MscI 958 TGGCCA TGG^CCA
MspI 29 CCGG C^CGG
MunI 587 CAATTG C^AATTG
NciI 29 CCGGG CC^[GC]GG
NdeI 1473 CATATG CA^TATG
NlaIII 1195 CATG CATG^
NspV 1372 TTCGAA TT^CGAA
PflFI 1356 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 913 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 994 GACTC GAGTC(4/5)
SfuI 1372 TTCGAA TT^CGAA
SspI 267 AATATT AAT^ATT
TaqI 1373 TCGA T^CGA
Tth111I 1356 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1373 TCGA T^CGA
Van91I 913 CCAATGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 34 enzymes) #
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AcsI 562 GAATTT [GA]^AATT[CT]
1390 AAATTC
AcyI 43 GGCGCC G[GA]^CG[CT]C
1241 GGCGCC
AfaI 1010 GTAC GT^AC
1426 GTAC
AlwI 301 GATCC GGATC(4/5)
910 GATCC
ApoI 562 GAATTT [GA]^AATT[CT]
1390 AAATTC
Asp700I 544 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
559 GAAGAATTTC
BbeI 43 GGCGCC GGCGC^C
1241 GGCGCC
BfaI 811 CTAG C^TAG
1006 CTAG
BpmI 76 CTGGAG CTGGAG(16/14)
514 CTGGAG
BsaHI 43 GGCGCC G[GA]^CG[CT]C
1241 GGCGCC
BsgI 57 CTGCAC GTGCAG(16/14)
665 CTGCAC
BsmFI 476 GGGAC GGGAC(10/14)
1016 GGGAC
BstF5I 1269 CATCC GGATG(2/0)
715 GGATG
Cfr13I 26 GGTCC G^G[ACGT]CC
31 GGGCC
EaeI 116 CGGCCA [CT]^GGCC[GA]
958 TGGCCA
EarI 1497 CTCTTC CTCTTC(1/4)
466 GAAGAG
FauI 64 GCGGG CCCGC(4/6)
102 GCGGG
FokI 1269 CATCC GGATG(9/13)
715 GGATG
HaeII 43 GGCGCC [GA]GCGC^[CT]
1241 GGCGCC
Hin1I 43 GGCGCC G[GA]^CG[CT]C
1241 GGCGCC
KasI 43 GGCGCC G^GCGCC
1241 GGCGCC
Ksp632I 1497 CTCTTC CTCTTC(1/4)
466 GAAGAG
MaeI 811 CTAG C^TAG
1006 CTAG
MslI 760 CATCTAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1294 CACACAGATG
MspA1I 484 CAGCTG C[AC]G^C[GT]G
836 CAGCTG
NarI 43 GGCGCC GG^CGCC
1241 GGCGCC
PvuII 484 CAGCTG CAG^CTG
836 CAGCTG
RsaI 1010 GTAC GT^AC
1426 GTAC
Sau96I 26 GGTCC G^G[ACGT]CC
31 GGGCC
SfoI 43 GGCGCC GGC^GCC
1241 GGCGCC
SmlI 729 CTTGAG C^T[CT][GA]AG
1039 CTTGAG
StuI 656 AGGCCT AGG^CCT
1035 AGGCCT
Tsp45I 1292 GTCAC ^GT[GC]AC
1407 GTCAC
XmnI 544 GAAAAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
559 GAAGAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AccII AciI AluI BanI BbvI
BsaJI BsiYI BslI BsmI BsrDI
BsrI BssKI BstNI BstUI BstYI
Cac8I CfoI DdeI DpnII EcoRII
EcoT14I Fnu4HI HaeIII HhaI HinP1I
HinfI HphI ItaI MaeIII MboI
MboII MflI MnlI MseI MvaI
MvnI MwoI NdeII NlaIV PstI
Sau3AI ScrFI SfaNI SfcI StyI
TfiI Tru9I TseI Tsp509I TspRI
XhoII