Restriction map for
pFN21AB5794
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI Asp718I AspEI AspI
AviII AvrII BbrPI BbvCI BcgI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaI BsaWI
BseAI BsiWI BsmBI Bsp1407I BspEI
BspLU11I BspMI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I CelII CpoI DraI DrdI
Eam1105I Eco47III Eco81I EcoO65I EcoRV
FbaI FseI FspI HindIII KpnI
MluI MroI NcoI NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacI SapI
ScaI SexAI SfiI SfuI SnaBI
SpeI SphI SspBI SspI SwaI
Tth111I XcmI XhoI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AccI 473 GTCGAC GT^[AC][GT]AC
AccII 93 CGCG CG^CG
AflIII 2215 ACGTGT A^C[GA][CT]GT
ApaI 179 GGGCCC GGGCC^C
AseI 1168 ATTAAT AT^TAAT
AsnI 1168 ATTAAT AT^TAAT
Asp700I 2257 GAATCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1500 GAGCAC G[AT]GC[AT]^C
BalI 1292 TGGCCA TGG^CCA
BamHI 152 GGATCC G^GATCC
BbeI 49 GGCGCC GGCGC^C
BciVI 2219 GTATCC GTATCC(6/5)
BsaBI 2031 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 297 GAGGAG GAGGAG(10/8)
BsiEI 95 CGGCCG CG[GA][CT]^CG
BsiHKAI 1500 GAGCAC G[AT]GC[AT]^C
BsmI 1946 GCATTC GAATGC(1/-1)
BspDI 2259 ATCGAT AT^CGAT
BsrBI 217 GAGCGG CCGCTC(-3/-3)
BssSI 172 CACGAG CACGAG(-5/-1)
BstUI 93 CGCG CG^CG
CfoI 50 GCGC GCG^C
ClaI 2259 ATCGAT AT^CGAT
DraIII 1811 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 92 CCGCGG C^C[GA][CT]GG
EagI 95 CGGCCG C^GGCCG
EclXI 95 CGGCCG C^GGCCG
Eco52I 95 CGGCCG C^GGCCG
EcoNI 1690 CCTCTGCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2227 GAATTC G^AATTC
EcoT14I 62 CCAAGG C^C[AT][AT]GG
EcoT22I 1340 ATGCAT ATGCA^T
HaeII 49 GGCGCC [GA]GCGC^[CT]
HhaI 50 GCGC GCG^C
HinP1I 50 GCGC G^CGC
HpaI 669 GTTAAC GTT^AAC
KasI 49 GGCGCC G^GCGCC
KspI 92 CCGCGG CCGC^GG
MamI 2031 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 794 CAATTG C^AATTG
MluNI 1292 TGGCCA TGG^CCA
MscI 1292 TGGCCA TGG^CCA
MunI 794 CAATTG C^AATTG
MvnI 93 CGCG CG^CG
NarI 49 GGCGCC GG^CGCC
NdeI 1885 CATATG CA^TATG
NsiI 1340 ATGCAT ATGCA^T
PflMI 2097 CCACTCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 15 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 940 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1168 ATTAAT AT^TAAT
PvuII 719 CAGCTG CAG^CTG
SacII 92 CCGCGG CCGC^GG
SalI 473 GTCGAC G^TCGAC
SbfI 249 CCTGCAGG CCTGCA^GG
SfoI 49 GGCGCC GGC^GCC
SgrAI 51 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 994 CTCAAG C^T[CT][GA]AG
Sse8387I 249 CCTGCAGG CCTGCA^GG
StuI 1281 AGGCCT AGG^CCT
StyI 62 CCAAGG C^C[AT][AT]GG
Van91I 2097 CCACTCGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2257 GAATCGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 22 GGCGTC G[GA]^CG[CT]C
49 GGCGCC
AfaI 138 GTAC GT^AC
1604 GTAC
AlwNI 626 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
674 CAGATTCTG
BanI 49 GGCGCC G^G[CT][GA]CC
58 GGTGCC
BsaHI 22 GGCGTC G[GA]^CG[CT]C
49 GGCGCC
BsrFI 52 GCCGGC [GA]^CCGG[CT]
97 GCCGGC
BstAPI 159 GCACTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
649 GCAATGATTGC
Cfr10I 52 GCCGGC [GA]^CCGG[CT]
97 GCCGGC
DraII 15 GGGTCCC [GA]G^G[ACGT]CC[CT]
1686 AGGCCCT
EaeI 95 CGGCCG [CT]^GGCC[GA]
1292 TGGCCA
EcoO109I 15 GGGTCCC [GA]G^G[ACGT]CC[CT]
1686 AGGCCCT
Hin1I 22 GGCGTC G[GA]^CG[CT]C
49 GGCGCC
HincII 473 GTCGAC GT[CT]^[GA]AC
669 GTTAAC
HindII 473 GTCGAC GT[CT]^[GA]AC
669 GTTAAC
MaeII 2190 ACGT A^CGT
2215 ACGT
NaeI 52 GCCGGC GCC^GGC
97 GCCGGC
NgoMIV 52 GCCGGC G^CCGGC
97 GCCGGC
RsaI 138 GTAC GT^AC
1604 GTAC
SmaI 45 CCCGGG CCC^GGG
147 CCCGGG
XmaCI 45 CCCGGG C^CCGGG
147 CCCGGG
XmaI 45 CCCGGG C^CCGGG
147 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BsgI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspHI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RcaI Sau3AI Sau96I ScrFI SfaNI
SfcI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XhoII