Restriction map for
pFN21AE2474
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# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AocI AscI AseI AsnI Asp718I
AviII AvrII BbeI BbrPI BcgI
BclI BfrI BglII BlnI BsaAI
BsiWI BsmBI BspDI BspLU11I BspMI
BsrBI BssHII BssSI Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DraI
Eco81I EcoRV FbaI FspI HpaI
KasI KpnI KspI MluI MseI
NarI NdeI NheI NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tru9I
XbaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 2295 GTCTAC GT^[AC][GT]AC
AccII 3736 CGCG CG^CG
AflIII 1454 ACGTGT A^C[GA][CT]GT
AhdI 3263 GACAGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1524 GTGCAC G^TGCAC
Aor51HI 3247 AGCGCT AGC^GCT
ApaLI 1524 GTGCAC G^TGCAC
AspEI 3263 GACAGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3346 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 707 GGATAC GTATCC(6/5)
BsaBI 3183 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2407 GAGACC GGTCTC(1/5)
BsiEI 2460 CGGCCG CG[GA][CT]^CG
BstUI 3736 CGCG CG^CG
DraIII 3945 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 2460 CGGCCG C^GGCCG
Eam1105I 3263 GACAGGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2460 CGGCCG C^GGCCG
Eco47III 3247 AGCGCT AGC^GCT
Eco52I 2460 CGGCCG C^GGCCG
EcoRI 861 GAATTC G^AATTC
FseI 1723 GGCCGGCC GGCCGG^CC
HaeII 3247 AGCGCT [GA]GCGC^[CT]
HincII 2953 GTTGAC GT[CT]^[GA]AC
HindII 2953 GTTGAC GT[CT]^[GA]AC
MamI 3183 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2093 CAATTG C^AATTG
MunI 2093 CAATTG C^AATTG
MvnI 3736 CGCG CG^CG
NaeI 1724 GCCGGC GCC^GGC
NcoI 404 CCATGG C^CATGG
NgoMIV 1724 GCCGGC G^CCGGC
PaeR7I 3845 CTCGAG C^TCGAG
PflFI 3346 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 2238 GACTC GAGTC(4/5)
SapI 1677 GCTCTTC GCTCTTC(1/4)
SfiI 1098 GGCCAGTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1835 CCCGGG CCC^GGG
SphI 2477 GCATGC GCATG^C
Tth111I 3346 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3845 CTCGAG C^TCGAG
XmaCI 1835 CCCGGG C^CCGGG
XmaI 1835 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 383 TCCGGA T^CCGGA
479 TCCGGA
AcsI 861 GAATTC [GA]^AATT[CT]
1943 GAATTT
AcyI 760 GACGCC G[GA]^CG[CT]C
1035 GGCGTC
ApaI 1838 GGGCCC GGGCC^C
2749 GGGCCC
ApoI 861 GAATTC [GA]^AATT[CT]
1943 GAATTT
Asp700I 420 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3444 GAACCCCTTC
BamHI 612 GGATCC G^GATCC
848 GGATCC
BanI 1132 GGCACC G^G[CT][GA]CC
1689 GGTGCC
BbvCI 1536 CCTCAGC CCTCAGC(-5/-2)
648 GCTGAGG
BglI 214 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1099 GCCAGTGGGGC
BsaHI 760 GACGCC G[GA]^CG[CT]C
1035 GGCGTC
BseAI 383 TCCGGA T^CCGGA
479 TCCGGA
BspEI 383 TCCGGA T^CCGGA
479 TCCGGA
BspHI 362 TCATGA T^CATGA
2552 TCATGA
BsrFI 1724 GCCGGC [GA]^CCGG[CT]
2458 ACCGGC
BstAPI 294 GCATCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3920 GCAATGCCTGC
BstEII 1299 GGTAACC G^GT[ACGT]ACC
1824 GGTCACC
BstPI 1299 GGTAACC G^GT[ACGT]ACC
1824 GGTCACC
BstXI 2429 CCACGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3603 CCAGGGCCTTGG
CfoI 1002 GCGC GCG^C
3248 GCGC
Cfr10I 1724 GCCGGC [GA]^CCGG[CT]
2458 ACCGGC
DrdI 556 GACCACTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1116 GACACTTGTGTC
EcoO65I 1299 GGTAACC G^GT[ACGT]ACC
1824 GGTCACC
EcoT22I 2475 ATGCAT ATGCA^T
3764 ATGCAT
FauI 143 GCGGG CCCGC(4/6)
338 GCGGG
HgaI 760 GACGC GACGC(5/10)
1036 GCGTC
HhaI 1002 GCGC GCG^C
3248 GCGC
Hin1I 760 GACGCC G[GA]^CG[CT]C
1035 GGCGTC
HinP1I 1002 GCGC G^CGC
3248 GCGC
MroI 383 TCCGGA T^CCGGA
479 TCCGGA
NsiI 2475 ATGCAT ATGCA^T
3764 ATGCAT
PflMI 1424 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2897 CCAGATGCTGG
RcaI 362 TCATGA T^CATGA
2552 TCATGA
Van91I 1424 CCAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2897 CCAGATGCTGG
XcmI 209 CCAATGCCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
764 CCAAGCTGGTCGTGG
XmnI 420 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3444 GAACCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BalI BanII
BbsI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CelII Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StuI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII