Restriction map for
pFN21AE1802
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AviII AvrII
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HincII HindII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MflI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
TthHB8I Van91I XbaI XcmI XhoI
XhoII
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# ONE CUT ENZYMES ( 56 enzymes) #
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AclI 593 AACGTT AA^CGTT
AfaI 423 GTAC GT^AC
AlwI 820 GATCC GGATC(4/5)
Asp700I 427 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 182 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 653 CCCGGG C^[CT]CG[GA]G
BalI 557 TGGCCA TGG^CCA
BbsI 726 GTCTTC GAAGAC(2/6)
BfaI 662 CTAG C^TAG
BpmI 456 CTGGAG CTGGAG(16/14)
BpuAI 726 GTCTTC GAAGAC(2/6)
BseRI 547 GAGGAG GAGGAG(10/8)
BsiYI 495 CCAGCTCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 495 CCAGCTCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 25 GAGAC GTCTC(1/5)
BsoBI 653 CCCGGG C^[CT]CG[GA]G
BsrDI 561 CATTGC GCAATG(2/0)
BstF5I 263 GGATG GGATG(2/0)
BstNI 356 CCAGG CC^[AT]GG
DdeI 779 CTCAG C^T[ACGT]AG
DraI 223 TTTAAA TTT^AAA
DraIII 686 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 436 CCATGG C^C[GA][CT]GG
EaeI 557 TGGCCA [CT]^GGCC[GA]
EcoRII 356 CCAGG ^CC[AT]GG
EcoT14I 436 CCATGG C^C[AT][AT]GG
FokI 263 GGATG GGATG(9/13)
HaeIII 558 GGCC GG^CC
HapII 654 CCGG C^CGG
HindIII 830 AAGCTT A^AGCTT
HinfI 784 GACTC G^A[ACGT]TC
HpaII 654 CCGG C^CGG
MaeI 662 CTAG C^TAG
MaeII 594 ACGT A^CGT
MluNI 557 TGGCCA TGG^CCA
MscI 557 TGGCCA TGG^CCA
MslI 604 CATCACAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 646 CAGCTG C[AC]G^C[GT]G
MspI 654 CCGG C^CGG
MvaI 356 CCAGG CC^[AT]GG
MwoI 81 GCCTAAAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 436 CCATGG C^CATGG
NlaIII 437 CATG CATG^
PflFI 182 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 784 GACTC GAGTC(4/5)
Psp1406I 593 AACGTT AA^CGTT
PvuII 646 CAGCTG CAG^CTG
RsaI 423 GTAC GT^AC
SfcI 516 CTATAG C^T[GA][CT]AG
SmaI 653 CCCGGG CCC^GGG
SmlI 827 CTCAAG C^T[CT][GA]AG
StyI 436 CCATGG C^C[AT][AT]GG
Tth111I 182 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 653 CCCGGG C^CCGGG
XmaI 653 CCCGGG C^CCGGG
XmnI 427 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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BbvI 272 GCTGC GCAGC(8/12)
648 GCTGC
BcnI 653 CCCGG CC^[GC]GG
654 CCGGG
BsmFI 491 GGGAC GGGAC(10/14)
657 GGGAC
Cac8I 129 GCAAGC GC[ACGT]^[ACGT]GC
533 GCAGGC
DpnII 71 GATC ^GATC
820 GATC
DraII 180 AGGACCT [GA]G^G[ACGT]CC[CT]
657 GGGACCT
EcoO109I 180 AGGACCT [GA]G^G[ACGT]CC[CT]
657 GGGACCT
Fnu4HI 272 GCTGC GC^[ACGT]GC
648 GCTGC
HgaI 105 GCGTC GACGC(5/10)
201 GCGTC
HphI 762 GGTGA GGTGA(8/7)
817 GGTGA
ItaI 272 GCTGC GC^[ACGT]GC
648 GCTGC
MaeIII 40 GTCAC ^GT[ACGT]AC
763 GTGAC
MboI 71 GATC ^GATC
820 GATC
NciI 653 CCCGG CC^[GC]GG
654 CCGGG
NdeII 71 GATC ^GATC
820 GATC
NlaIV 491 GGGACC GG[ACGT]^[ACGT]CC
657 GGGACC
PpuMI 180 AGGACCT [GA]G^G[AT]CC[CT]
657 GGGACCT
Sau3AI 71 GATC ^GATC
820 GATC
TseI 272 GCTGC G^C[AT]GC
648 GCTGC
Tsp45I 40 GTCAC ^GT[GC]AC
763 GTGAC
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# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AcsI AluI ApoI AvaII BsaJI
BsrI BssKI Cfr13I MboII MnlI
MseI Sau96I ScrFI Tru9I Tsp509I
TspRI