Restriction map for
pFN21AE1898
##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BanII
BcgI BclI BfrI BglI BlnI
BsaBI BsaI BseRI BsiEI BsiWI
BsmBI BspDI BsrBI BsrDI BsrFI
BssHII Bst1107I BstBI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI FbaI FseI FspI
HpaI KpnI KspI MamI MfeI
MluI MluNI MscI MunI NaeI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmeI PshAI
PvuI RsrII SacI SacII SalI
SbfI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I XbaI XcmI XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 598 GTAGAC GT^[AC][GT]AC
AccII 53 CGCG CG^CG
AclI 263 AACGTT AA^CGTT
AcsI 276 AAATTT [GA]^AATT[CT]
ApoI 276 AAATTT [GA]^AATT[CT]
AseI 1612 ATTAAT AT^TAAT
AsnI 1612 ATTAAT AT^TAAT
AspHI 1155 GAGCAC G[AT]GC[AT]^C
BbsI 2290 GAAGAC GAAGAC(2/6)
BbvCI 976 GCTGAGG CCTCAGC(-5/-2)
BciVI 1305 GTATCC GTATCC(6/5)
BglII 174 AGATCT A^GATCT
BpuAI 2290 GAAGAC GAAGAC(2/6)
BsiHKAI 1155 GAGCAC G[AT]GC[AT]^C
Bsp1407I 1888 TGTACA T^GTACA
BspHI 1212 TCATGA T^CATGA
BspLU11I 192 ACATGT A^CATGT
BsrGI 1888 TGTACA T^GTACA
BssSI 1330 CACGAG CACGAG(-5/-1)
BstEII 2397 GGTCACC G^GT[ACGT]ACC
BstPI 2397 GGTCACC G^GT[ACGT]ACC
BstUI 53 CGCG CG^CG
DrdI 2389 GACCATCCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1080 CCATGG C^C[GA][CT]GG
EaeI 1824 TGGCCG [CT]^GGCC[GA]
EcoNI 1182 CCTTACAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2397 GGTCACC G^GT[ACGT]ACC
EcoRV 511 GATATC GAT^ATC
EcoT14I 1080 CCATGG C^C[AT][AT]GG
EcoT22I 1731 ATGCAT ATGCA^T
FauI 1665 CCCGC CCCGC(4/6)
HgaI 2436 GCGTC GACGC(5/10)
HincII 2449 GTTGAC GT[CT]^[GA]AC
HindII 2449 GTTGAC GT[CT]^[GA]AC
HindIII 100 AAGCTT A^AGCTT
MvnI 53 CGCG CG^CG
NcoI 1080 CCATGG C^CATGG
NdeI 2311 CATATG CA^TATG
NsiI 1731 ATGCAT ATGCA^T
PshBI 1612 ATTAAT AT^TAAT
Psp1406I 263 AACGTT AA^CGTT
RcaI 1212 TCATGA T^CATGA
ScaI 256 AGTACT AGT^ACT
SmaI 69 CCCGGG CCC^GGG
SspBI 1888 TGTACA T^GTACA
StyI 1080 CCATGG C^C[AT][AT]GG
XmaCI 69 CCCGGG C^CCGGG
XmaI 69 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 60 TCCGGA T^CCGGA
1248 TCCGGA
AcyI 48 GGCGCC G[GA]^CG[CT]C
2241 GGCGCC
AlwNI 1509 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
1756 CAGCAGCTG
BanI 48 GGCGCC G^G[CT][GA]CC
2241 GGCGCC
BbeI 48 GGCGCC GGCGC^C
2241 GGCGCC
BbrPI 352 CACGTG CAC^GTG
669 CACGTG
BcnI 69 CCCGG CC^[GC]GG
70 CCGGG
BlpI 1974 GCTGAGC GC^T[ACGT]AGC
2145 GCTGAGC
Bpu1102I 1974 GCTGAGC GC^T[ACGT]AGC
2145 GCTGAGC
BsaAI 352 CACGTG [CT]AC^GT[GA]
669 CACGTG
BsaHI 48 GGCGCC G[GA]^CG[CT]C
2241 GGCGCC
BseAI 60 TCCGGA T^CCGGA
1248 TCCGGA
BsmFI 1221 GGGAC GGGAC(10/14)
1814 GGGAC
BspEI 60 TCCGGA T^CCGGA
1248 TCCGGA
BspMI 1498 ACCTGC ACCTGC(4/8)
808 GCAGGT
CelII 1974 GCTGAGC GC^T[ACGT]AGC
2145 GCTGAGC
DraII 1814 GGGACCT [GA]G^G[ACGT]CC[CT]
2336 AGGACCC
EcoO109I 1814 GGGACCT [GA]G^G[ACGT]CC[CT]
2336 AGGACCC
HaeII 48 GGCGCC [GA]GCGC^[CT]
2241 GGCGCC
Hin1I 48 GGCGCC G[GA]^CG[CT]C
2241 GGCGCC
KasI 48 GGCGCC G^GCGCC
2241 GGCGCC
MroI 60 TCCGGA T^CCGGA
1248 TCCGGA
MslI 1081 CATGGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1311 CATAGCTGTG
NarI 48 GGCGCC GG^CGCC
2241 GGCGCC
NciI 69 CCCGG CC^[GC]GG
70 CCGGG
NspI 192 ACATGT [GA]CATG^[CT]
1774 GCATGT
PaeR7I 94 CTCGAG C^TCGAG
1440 CTCGAG
PflMI 2073 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2268 CCAGAGAATGG
PmaCI 352 CACGTG CAC^GTG
669 CACGTG
PmlI 352 CACGTG CAC^GTG
669 CACGTG
PpuMI 1814 GGGACCT [GA]G^G[AT]CC[CT]
2336 AGGACCC
SapI 616 GAAGAGC GCTCTTC(1/4)
641 GAAGAGC
SexAI 876 ACCTGGT A^CC[AT]GGT
1199 ACCTGGT
SfoI 48 GGCGCC GGC^GCC
2241 GGCGCC
Van91I 2073 CCATTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2268 CCAGAGAATGG
XhoI 94 CTCGAG C^TCGAG
1440 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AvaI AvaII BbvI BfaI BmyI
BpmI BsaJI BsaWI BsgI BsiYI
BslI BsmAI BsmI BsoBI Bsp1286I
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI EarI EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII