Restriction map for
pFN21AE4130
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI AlwNI AocI Aor51HI
ApaI AscI Asp718I AspI AviII
AvrII BbeI BbrPI BciVI BfrI
BglI BlnI BsaAI BsaHI BsaI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraIII DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoT14I EcoT22I FseI
FspI HaeII Hin1I HincII HindII
HpaI KasI KpnI KspI MluI
MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 998 GTCTAC GT^[AC][GT]AC
AhdI 1522 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1277 GTGCAC G^TGCAC
ApaLI 1277 GTGCAC G^TGCAC
AspEI 1522 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1277 GTGCAC G[AT]GC[AT]^C
BalI 1086 TGGCCA TGG^CCA
BanII 1398 GGGCTC G[GA]GC[CT]^C
BbvCI 655 GCTGAGG CCTCAGC(-5/-2)
BbvI 663 GCTGC GCAGC(8/12)
BcgI 1499 GCAGAGAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 982 CTAG C^TAG
BglII 771 AGATCT A^GATCT
BsaBI 796 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 410 GTGCAG GTGCAG(16/14)
BsiHKAI 1277 GTGCAC G[AT]GC[AT]^C
BsmBI 1054 GAGACG CGTCTC(1/5)
BspMI 824 ACCTGC ACCTGC(4/8)
BsrDI 519 CATTGC GCAATG(2/0)
CfoI 1060 GCGC GCG^C
DraI 175 TTTAAA TTT^AAA
DraII 99 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 92 CCGTGG C^C[GA][CT]GG
EaeI 1086 TGGCCA [CT]^GGCC[GA]
Eam1105I 1522 GACCTAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 35 GAAGAG CTCTTC(1/4)
EcoO109I 99 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 1228 GAATTC G^AATTC
EcoRV 954 GATATC GAT^ATC
FauI 676 GCGGG CCCGC(4/6)
Fnu4HI 663 GCTGC GC^[ACGT]GC
HgaI 1098 GACGC GACGC(5/10)
HhaI 1060 GCGC GCG^C
HinP1I 1060 GCGC G^CGC
HindIII 1160 AAGCTT A^AGCTT
ItaI 663 GCTGC GC^[ACGT]GC
Ksp632I 35 GAAGAG CTCTTC(1/4)
MaeI 982 CTAG C^TAG
MamI 796 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 314 CAATTG C^AATTG
MluNI 1086 TGGCCA TGG^CCA
MscI 1086 TGGCCA TGG^CCA
MunI 314 CAATTG C^AATTG
PpuMI 99 AGGACCC [GA]G^G[AT]CC[CT]
SexAI 932 ACCTGGT A^CC[AT]GGT
SfaNI 886 GATGC GCATC(5/9)
SmlI 706 CTTGAG C^T[CT][GA]AG
StuI 687 AGGCCT AGG^CCT
TfiI 1116 GATTC G^A[AT]TC
TseI 663 GCTGC G^C[AT]GC
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# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 675 CGCG CG^CG
1061 CGCG
AccIII 882 TCCGGA T^CCGGA
1203 TCCGGA
AseI 789 ATTAAT AT^TAAT
843 ATTAAT
AsnI 789 ATTAAT AT^TAAT
843 ATTAAT
Asp700I 602 GAACGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
863 GAATTTCTTC
AvaI 130 CCCGAG C^[CT]CG[GA]G
1123 CCCGAG
AvaII 100 GGACC G^G[AT]CC
280 GGACC
BamHI 199 GGATCC G^GATCC
1200 GGATCC
BanI 185 GGCACC G^G[CT][GA]CC
680 GGTGCC
BbsI 1095 GAAGAC GAAGAC(2/6)
1439 GTCTTC
BclI 525 TGATCA T^GATCA
1368 TGATCA
BcnI 1396 CCGGG CC^[GC]GG
1495 CCGGG
BlpI 375 GCTGAGC GC^T[ACGT]AGC
1141 GCTGAGC
Bpu1102I 375 GCTGAGC GC^T[ACGT]AGC
1141 GCTGAGC
BpuAI 1095 GAAGAC GAAGAC(2/6)
1439 GTCTTC
BseAI 882 TCCGGA T^CCGGA
1203 TCCGGA
BseRI 437 GAGGAG GAGGAG(10/8)
1126 GAGGAG
BsmAI 334 GAGAC GTCTC(1/5)
1054 GAGAC
BsoBI 130 CCCGAG C^[CT]CG[GA]G
1123 CCCGAG
BspEI 882 TCCGGA T^CCGGA
1203 TCCGGA
BstUI 675 CGCG CG^CG
1061 CGCG
CelII 375 GCTGAGC GC^T[ACGT]AGC
1141 GCTGAGC
Cfr13I 100 GGACC G^G[ACGT]CC
280 GGACC
FbaI 525 TGATCA T^GATCA
1368 TGATCA
HphI 427 GGTGA GGTGA(8/7)
1419 GGTGA
MroI 882 TCCGGA T^CCGGA
1203 TCCGGA
MvnI 675 CGCG CG^CG
1061 CGCG
NciI 1396 CCGGG CC^[GC]GG
1495 CCGGG
NspI 57 ACATGC [GA]CATG^[CT]
451 GCATGT
PshBI 789 ATTAAT AT^TAAT
843 ATTAAT
Sau96I 100 GGACC G^G[ACGT]CC
280 GGACC
TaqI 33 TCGA T^CGA
1508 TCGA
Tsp45I 5 GTGAC ^GT[GC]AC
1434 GTCAC
TthHB8I 33 TCGA T^CGA
1508 TCGA
XmnI 602 GAACGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
863 GAATTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI BmyI BpmI BsaJI BsaWI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
DdeI DpnII EcoRII FokI HaeIII
HapII HinfI HpaII MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PleI PvuII RsaI
Sau3AI ScrFI Tru9I Tsp509I TspRI
XhoII