Restriction map for
pFN21AE6286
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbvCI BbvI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaI BsaJI BsaWI
BseAI BsgI BsiEI BsiWI BsiYI
BslI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I ClaI CpoI DraII
DraIII DrdI DsaI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I FauI
FbaI Fnu4HI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
ItaI KasI KpnI KspI MaeI
MflI MluI MroI MslI MspA1I
MspI MvnI NaeI NarI NciI
NcoI NgoMIV NheI NlaIV NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TseI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 729 GTATAC GT^[AC][GT]AC
AciI 597 GCGG CCGC(-3/-1)
AcsI 797 AAATTC [GA]^AATT[CT]
AfaI 527 GTAC GT^AC
AhdI 536 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 16 GGATC GGATC(4/5)
ApoI 797 AAATTC [GA]^AATT[CT]
AspEI 536 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 236 GTGCTC G[AT]GC[AT]^C
AvaII 703 GGACC G^G[AT]CC
BalI 749 TGGCCA TGG^CCA
BbsI 469 GAAGAC GAAGAC(2/6)
BmyI 236 GTGCTC G[AGT]GC[ACT]^C
BpmI 584 CTGGAG CTGGAG(16/14)
BpuAI 469 GAAGAC GAAGAC(2/6)
BsaBI 334 GATATGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 236 GTGCTC G[AT]GC[AT]^C
BsmFI 626 GGGAC GGGAC(10/14)
BsmI 762 GAATGC GAATGC(1/-1)
Bsp1286I 236 GTGCTC G[AGT]GC[ACT]^C
BssKI 306 CCAGG ^CC[ACGT]GG
Bst1107I 729 GTATAC GTA^TAC
BstNI 306 CCAGG CC^[AT]GG
BstZ17I 729 GTATAC GTA^TAC
Cfr13I 703 GGACC G^G[ACGT]CC
DraI 115 TTTAAA TTT^AAA
EaeI 749 TGGCCA [CT]^GGCC[GA]
Eam1105I 536 GACTGAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRII 306 CCAGG ^CC[AT]GG
EcoT22I 337 ATGCAT ATGCA^T
HphI 97 TCACC GGTGA(8/7)
MaeII 663 ACGT A^CGT
MaeIII 544 GTCAC ^GT[ACGT]AC
MamI 334 GATATGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 451 CAATTG C^AATTG
MluNI 749 TGGCCA TGG^CCA
MscI 749 TGGCCA TGG^CCA
MunI 451 CAATTG C^AATTG
MvaI 306 CCAGG CC^[AT]GG
MwoI 766 GCTTGTTATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NdeI 808 CATATG CA^TATG
NsiI 337 ATGCAT ATGCA^T
PleI 542 GAGTC GAGTC(4/5)
RsaI 527 GTAC GT^AC
Sau96I 703 GGACC G^G[ACGT]CC
ScrFI 306 CCAGG CC^[ACGT]GG
TfiI 265 GAATC G^A[AT]TC
Tsp45I 544 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 13 enzymes) #
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AseI 423 ATTAAT AT^TAAT
442 ATTAAT
AsnI 423 ATTAAT AT^TAAT
442 ATTAAT
BseRI 217 CTCCTC GAGGAG(10/8)
498 CTCCTC
EarI 68 GAAGAG CTCTTC(1/4)
856 GAAGAG
HaeIII 111 GGCC GG^CC
750 GGCC
HinfI 265 GAATC G^A[ACGT]TC
542 GAGTC
Ksp632I 68 GAAGAG CTCTTC(1/4)
856 GAAGAG
NlaIII 43 CATG CATG^
148 CATG
PshBI 423 ATTAAT AT^TAAT
442 ATTAAT
SfcI 362 CTACAG C^T[GA][CT]AG
370 CTACAG
TaqI 351 TCGA T^CGA
554 TCGA
TspRI 612 CAGTG CA[GC]TG[ACGT][ACGT]^
699 CACTG
TthHB8I 351 TCGA T^CGA
554 TCGA
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# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
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AluI BsmAI BsrI BstF5I DdeI
DpnII FokI MboI MboII MnlI
MseI NdeII Sau3AI SfaNI Tru9I
Tsp509I