Restriction map for
pFN21AE1979
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI ApaI ApaLI AscI
Asp700I AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BfrI BglII
BlnI BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrGI BssHII BssSI Bst1107I BstAPI
BstUI BstZ17I Bsu36I ClaI CpoI
DraII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO109I EcoRV
EcoT22I FseI FspI HgaI Hin1I
KasI KspI MaeII MamI MluI
MroI MvnI NaeI NarI NcoI
NdeI NgoMIV NotI NruI NsiI
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
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Acc65I 371 GGTACC G^GTACC
AlwNI 1305 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 122 AGCGCT AGC^GCT
AseI 1138 ATTAAT AT^TAAT
AsnI 1138 ATTAAT AT^TAAT
Asp718I 371 GGTACC G^GTACC
AspHI 742 GAGCAC G[AT]GC[AT]^C
AvaII 1461 GGACC G^G[AT]CC
BalI 1426 TGGCCA TGG^CCA
BanII 82 GAGCCC G[GA]GC[CT]^C
BclI 1086 TGATCA T^GATCA
BcnI 432 CCGGG CC^[GC]GG
BlpI 652 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 652 GCTGAGC GC^T[ACGT]AGC
BseRI 1400 GAGGAG GAGGAG(10/8)
BsgI 409 GTGCAG GTGCAG(16/14)
BsiHKAI 742 GAGCAC G[AT]GC[AT]^C
BspHI 546 TCATGA T^CATGA
BspMI 710 ACCTGC ACCTGC(4/8)
BsrBI 109 GAGCGG CCGCTC(-3/-3)
BstBI 488 TTCGAA TT^CGAA
BstEII 1443 GGTGACC G^GT[ACGT]ACC
BstPI 1443 GGTGACC G^GT[ACGT]ACC
BstXI 65 CCATTTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1665 GGATCT [GA]^GATC[CT]
CelII 652 GCTGAGC GC^T[ACGT]AGC
CfoI 123 GCGC GCG^C
DsaI 1643 CCGTGG C^C[GA][CT]GG
Eco47III 122 AGCGCT AGC^GCT
EcoO65I 1443 GGTGACC G^GT[ACGT]ACC
EcoRI 51 GAATTC G^AATTC
FauI 1565 CCCGC CCCGC(4/6)
FbaI 1086 TGATCA T^GATCA
HaeII 122 AGCGCT [GA]GCGC^[CT]
HhaI 123 GCGC GCG^C
HinP1I 123 GCGC G^CGC
HincII 997 GTTAAC GT[CT]^[GA]AC
HindII 997 GTTAAC GT[CT]^[GA]AC
HindIII 909 AAGCTT A^AGCTT
HpaI 997 GTTAAC GTT^AAC
KpnI 371 GGTACC GGTAC^C
MflI 1665 GGATCT [GA]^GATC[CT]
MluNI 1426 TGGCCA TGG^CCA
MscI 1426 TGGCCA TGG^CCA
MslI 466 CATTTGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 432 CCGGG CC^[GC]GG
NheI 840 GCTAGC G^CTAGC
NspI 746 ACATGC [GA]CATG^[CT]
NspV 488 TTCGAA TT^CGAA
PflMI 99 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1411 GACTC GAGTC(4/5)
PshBI 1138 ATTAAT AT^TAAT
PstI 712 CTGCAG CTGCA^G
PvuII 1156 CAGCTG CAG^CTG
RcaI 546 TCATGA T^CATGA
SfuI 488 TTCGAA TT^CGAA
SmlI 971 CTCAAG C^T[CT][GA]AG
SspI 1141 AATATT AAT^ATT
TaqI 489 TCGA T^CGA
TfiI 543 GAATC G^A[AT]TC
TthHB8I 489 TCGA T^CGA
Van91I 99 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1665 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 51 GAATTC [GA]^AATT[CT]
1015 AAATTT
AlwI 378 GGATC GGATC(4/5)
1665 GGATC
ApoI 51 GAATTC [GA]^AATT[CT]
1015 AAATTT
BanI 182 GGCACC G^G[CT][GA]CC
371 GGTACC
BglI 1307 GCCGCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1428 GCCATTCAGGC
BpmI 1189 CTGGAG CTGGAG(16/14)
1660 CTGGAG
BsaI 1026 GAGACC GGTCTC(1/5)
1489 GAGACC
BsmAI 1026 GAGAC GTCTC(1/5)
1489 GAGAC
BsmI 1593 GAATGC GAATGC(1/-1)
726 GCATTC
BsrDI 1548 CATTGC GCAATG(2/0)
332 GCAATG
BsrFI 368 GCCGGT [GA]^CCGG[CT]
1319 ACCGGC
Cac8I 194 GCCAGC GC[ACGT]^[ACGT]GC
840 GCTAGC
Cfr10I 368 GCCGGT [GA]^CCGG[CT]
1319 ACCGGC
Cfr13I 366 GGGCC G^G[ACGT]CC
1461 GGACC
DraIII 145 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG
1270 CACACAGTG
EaeI 1426 TGGCCA [CT]^GGCC[GA]
1640 TGGCCG
EcoT14I 71 CCTTGG C^C[AT][AT]GG
353 CCAAGG
HinfI 543 GAATC G^A[ACGT]TC
1411 GACTC
MfeI 288 CAATTG C^AATTG
591 CAATTG
MspA1I 1156 CAGCTG C[AC]G^C[GT]G
1308 CCGCTG
MunI 288 CAATTG C^AATTG
591 CAATTG
Sau96I 366 GGGCC G^G[ACGT]CC
1461 GGACC
StyI 71 CCTTGG C^C[AT][AT]GG
353 CCAAGG
XcmI 168 CCAGACAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
353 CCAAGGTTATTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI BbvI BfaI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
DdeI DpnII DraI EarI EcoRII
Fnu4HI FokI HaeIII HapII HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
RsaI SapI Sau3AI ScrFI SfaNI
SfcI Tru9I TseI Tsp45I Tsp509I
TspRI