Restriction map for
pFN21AE2089
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI Asp700I AspEI AviII
AvrII BbeI BbvCI BciVI BfrI
BglII BlnI BsaBI BsaWI BseAI
BsgI BspDI BspEI BspHI BspLU11I
BsrBI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV EcoT22I FseI FspI HindIII
HpaI KasI KspI MamI MfeI
MluI MroI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SbfI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 1395 GGTACC G^GTACC
AccII 1711 CGCG CG^CG
AcyI 1303 GACGCC G[GA]^CG[CT]C
Aor51HI 1914 AGCGCT AGC^GCT
Asp718I 1395 GGTACC G^GTACC
AspI 838 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 114 CACGTG CAC^GTG
BcgI 1541 GCAAGGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 932 TGATCA T^GATCA
BsaAI 114 CACGTG [CT]AC^GT[GA]
BsaHI 1303 GACGCC G[GA]^CG[CT]C
BsiEI 2007 CGACCG CG[GA][CT]^CG
BsiWI 1469 CGTACG C^GTACG
BsmBI 219 CGTCTC CGTCTC(1/5)
Bsp1407I 494 TGTACA T^GTACA
BspMI 19 ACCTGC ACCTGC(4/8)
BsrDI 288 CATTGC GCAATG(2/0)
BsrGI 494 TGTACA T^GTACA
BstAPI 1732 GCAGACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1711 CGCG CG^CG
Eco47III 1914 AGCGCT AGC^GCT
EcoT14I 2204 CCAAGG C^C[AT][AT]GG
FbaI 932 TGATCA T^GATCA
Hin1I 1303 GACGCC G[GA]^CG[CT]C
KpnI 1395 GGTACC GGTAC^C
MseI 1214 TTAA T^TAA
MvnI 1711 CGCG CG^CG
NspI 2045 GCATGT [GA]CATG^[CT]
PflFI 838 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 114 CACGTG CAC^GTG
PmlI 114 CACGTG CAC^GTG
SalI 2005 GTCGAC G^TCGAC
SapI 6 GCTCTTC GCTCTTC(1/4)
SexAI 1595 ACCTGGT A^CC[AT]GGT
SfiI 2259 GGCCGCCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 494 TGTACA T^GTACA
StyI 2204 CCAAGG C^C[AT][AT]GG
Tru9I 1214 TTAA T^TAA
Tth111I 838 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccI 415 GTCTAC GT^[AC][GT]AC
2005 GTCGAC
BamHI 887 GGATCC G^GATCC
1800 GGATCC
BbsI 739 GAAGAC GAAGAC(2/6)
34 GTCTTC
BglI 2215 GCCGAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2260 GCCGCCGAGGC
BlpI 297 GCTGAGC GC^T[ACGT]AGC
633 GCTCAGC
Bpu1102I 297 GCTGAGC GC^T[ACGT]AGC
633 GCTCAGC
BpuAI 739 GAAGAC GAAGAC(2/6)
34 GTCTTC
BsaI 2170 GAGACC GGTCTC(1/5)
1920 GGTCTC
BsmI 471 GAATGC GAATGC(1/-1)
693 GAATGC
BstEII 441 GGTGACC G^GT[ACGT]ACC
1599 GGTGACC
BstPI 441 GGTGACC G^GT[ACGT]ACC
1599 GGTGACC
BstYI 887 GGATCC [GA]^GATC[CT]
1800 GGATCC
CelII 297 GCTGAGC GC^T[ACGT]AGC
633 GCTCAGC
DrdI 406 GACACTAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
838 GACGTGGTCGTC
EcoO65I 441 GGTGACC G^GT[ACGT]ACC
1599 GGTGACC
HgaI 1303 GACGC GACGC(5/10)
1735 GACGC
MflI 887 GGATCC [GA]^GATC[CT]
1800 GGATCC
PstI 997 CTGCAG CTGCA^G
1664 CTGCAG
PvuII 295 CAGCTG CAG^CTG
994 CAGCTG
ScaI 158 AGTACT AGT^ACT
1145 AGTACT
SmlI 1390 CTCAAG C^T[CT][GA]AG
1432 CTCAAG
XcmI 903 CCAGGACAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2081 CCAATGACAACATGG
XhoII 887 GGATCC [GA]^GATC[CT]
1800 GGATCC
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# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstXI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I