Restriction map for
pFN21AE4358
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AhdI Alw44I
AocI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BcgI BciVI
BclI BcnI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI HphI
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MseI MspA1I
MunI MvnI NaeI NarI NciI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tru9I Tsp45I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcyI 832 GGCGTC G[GA]^CG[CT]C
AgeI 767 ACCGGT A^CCGGT
AlwI 16 GGATC GGATC(4/5)
AlwNI 398 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 968 AGCGCT AGC^GCT
BanII 41 GGGCTC G[GA]GC[CT]^C
BbsI 3 GTCTTC GAAGAC(2/6)
BpmI 935 CTGGAG CTGGAG(16/14)
BpuAI 3 GTCTTC GAAGAC(2/6)
BsaBI 924 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 832 GGCGTC G[GA]^CG[CT]C
BsaWI 767 ACCGGT [AT]^CCGG[AT]
BsgI 536 GTGCAG GTGCAG(16/14)
BsmI 389 GCATTC GAATGC(1/-1)
BstYI 16 GGATCT [GA]^GATC[CT]
DpnII 17 GATC ^GATC
DraII 90 GGGACCC [GA]G^G[ACGT]CC[CT]
Eco47III 968 AGCGCT AGC^GCT
EcoO109I 90 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoT14I 227 CCATGG C^C[AT][AT]GG
FauI 192 GCGGG CCCGC(4/6)
HaeII 968 AGCGCT [GA]GCGC^[CT]
HgaI 833 GCGTC GACGC(5/10)
Hin1I 832 GGCGTC G[GA]^CG[CT]C
MaeII 270 ACGT A^CGT
MamI 924 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 17 GATC ^GATC
MflI 16 GGATCT [GA]^GATC[CT]
NcoI 227 CCATGG C^CATGG
NdeII 17 GATC ^GATC
NspI 157 ACATGC [GA]CATG^[CT]
PinAI 767 ACCGGT A^CCGGT
PleI 1061 GAGTC GAGTC(4/5)
PpuMI 90 GGGACCC [GA]G^G[AT]CC[CT]
Sau3AI 17 GATC ^GATC
SfaNI 380 GCATC GCATC(5/9)
SfcI 499 CTACAG C^T[GA][CT]AG
SmlI 825 CTTGAG C^T[CT][GA]AG
StyI 227 CCATGG C^C[AT][AT]GG
TaqI 957 TCGA T^CGA
TfiI 455 GAATC G^A[AT]TC
TthHB8I 957 TCGA T^CGA
XcmI 227 CCATGGAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 16 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AspHI 295 GTGCTC G[AT]GC[AT]^C
541 GTGCTC
BbvCI 549 CCTCAGC CCTCAGC(-5/-2)
862 GCTGAGG
BfaI 100 CTAG C^TAG
1093 CTAG
BglI 893 GCCAGATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1091 GCCTAGAAGGC
BseRI 181 GAGGAG GAGGAG(10/8)
184 GAGGAG
BsiHKAI 295 GTGCTC G[AT]GC[AT]^C
541 GTGCTC
Bsp1407I 251 TGTACA T^GTACA
926 TGTACA
BsrFI 767 ACCGGT [GA]^CCGG[CT]
788 ACCGGC
BsrGI 251 TGTACA T^GTACA
926 TGTACA
BstF5I 282 CATCC GGATG(2/0)
521 GGATG
CfoI 757 GCGC GCG^C
969 GCGC
Cfr10I 767 ACCGGT [GA]^CCGG[CT]
788 ACCGGC
DsaI 227 CCATGG C^C[GA][CT]GG
700 CCACGG
EcoRI 959 GAATTC G^AATTC
1078 GAATTC
FokI 282 CATCC GGATG(9/13)
521 GGATG
HapII 768 CCGG C^CGG
789 CCGG
HhaI 757 GCGC GCG^C
969 GCGC
HinP1I 757 GCGC G^CGC
969 GCGC
HinfI 455 GAATC G^A[ACGT]TC
1061 GAGTC
HpaII 768 CCGG C^CGG
789 CCGG
MaeI 100 CTAG C^TAG
1093 CTAG
MaeIII 53 GTAAC ^GT[ACGT]AC
1085 GTTAC
MspI 768 CCGG C^CGG
789 CCGG
NdeI 287 CATATG CA^TATG
993 CATATG
PflMI 222 CCACGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1020 CCATGTCCTGG
SapI 148 GAAGAGC GCTCTTC(1/4)
637 GCTCTTC
SspBI 251 TGTACA T^GTACA
926 TGTACA
Van91I 222 CCACGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1020 CCATGTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BmyI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstNI BstXI Cac8I Cfr13I
DdeI EarI EcoRII Fnu4HI HaeIII
ItaI Ksp632I MboII MnlI MslI
MvaI MwoI NlaIII NlaIV RsaI
Sau96I ScrFI TseI Tsp509I TspRI