Restriction map for
pFN21AE3883
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# NO CUT ENZYMES ( 190 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BlnI BlpI BmyI Bpu1102I
BpuAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CfoI
Cfr10I Cfr13I ClaI CpoI DraI
DraII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI Sau96I SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI Tsp45I Tth111I TthHB8I Van91I
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AfaI 73 GTAC GT^AC
AlwNI 179 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 628 ATTAAT AT^TAAT
AsnI 628 ATTAAT AT^TAAT
BbvI 181 GCTGC GCAGC(8/12)
BglII 24 AGATCT A^GATCT
BpmI 281 CTGGAG CTGGAG(16/14)
BsaAI 74 TACGTG [CT]AC^GT[GA]
BsgI 310 GTGCAG GTGCAG(16/14)
BsmBI 605 GAGACG CGTCTC(1/5)
BspMI 312 GCAGGT ACCTGC(4/8)
BsrI 280 ACTGG ACTGG(1/-1)
BstF5I 235 GGATG GGATG(2/0)
BstXI 404 CCATCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 660 GCAAGC GC[ACGT]^[ACGT]GC
DdeI 302 CTGAG C^T[ACGT]AG
DraIII 480 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 495 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI 181 GCTGC GC^[ACGT]GC
FokI 235 GGATG GGATG(9/13)
HphI 216 GGTGA GGTGA(8/7)
ItaI 181 GCTGC GC^[ACGT]GC
MaeII 75 ACGT A^CGT
MaeIII 371 GTTAC ^GT[ACGT]AC
NlaIII 378 CATG CATG^
PshBI 628 ATTAAT AT^TAAT
RsaI 73 GTAC GT^AC
SapI 10 GAAGAGC GCTCTTC(1/4)
SfcI 16 CTACAG C^T[GA][CT]AG
TseI 181 GCTGC G^C[AT]GC
XbaI 27 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcsI 590 AAATTT [GA]^AATT[CT]
679 GAATTT
AlwI 556 GATCC GGATC(4/5)
240 GGATC
ApoI 590 AAATTT [GA]^AATT[CT]
679 GAATTT
BsaJI 500 CCCAGG C^C[ACGT][ACGT]GG
638 CCTGGG
BsiYI 495 CCTGACCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
559 CCTAGTGAGGG
BslI 495 CCTGACCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
559 CCTAGTGAGGG
BssKI 501 CCAGG ^CC[ACGT]GG
638 CCTGG
BstNI 501 CCAGG CC^[AT]GG
638 CCTGG
BstYI 24 AGATCT [GA]^GATC[CT]
555 AGATCC
EcoRII 501 CCAGG ^CC[AT]GG
638 CCTGG
HinfI 354 GAATC G^A[ACGT]TC
646 GAATC
MflI 24 AGATCT [GA]^GATC[CT]
555 AGATCC
MseI 277 TTAA T^TAA
629 TTAA
MslI 378 CATGATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
405 CATCTCTGTG
MspA1I 89 CAGCTG C[AC]G^C[GT]G
179 CAGCTG
MvaI 501 CCAGG CC^[AT]GG
638 CCTGG
MwoI 82 GCCCTTACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
184 GCTGTGGTTGC
PvuII 89 CAGCTG CAG^CTG
179 CAGCTG
ScrFI 501 CCAGG CC^[ACGT]GG
638 CCTGG
SfaNI 79 GATGC GCATC(5/9)
129 GATGC
TfiI 354 GAATC G^A[AT]TC
646 GAATC
Tru9I 277 TTAA T^TAA
629 TTAA
TspRI 300 CACTG CA[GC]TG[ACGT][ACGT]^
546 CACTG
XhoII 24 AGATCT [GA]^GATC[CT]
555 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
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AluI BfaI BsmAI DpnII EarI
Ksp632I MaeI MboI MboII MnlI
NdeII Sau3AI Tsp509I