Restriction map for
pFN21AE1607
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# NO CUT ENZYMES ( 204 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AfaI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbsI BbvCI BbvI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsiYI BslI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I ClaI
CpoI DdeI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI Fnu4HI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HpaI HpaII
ItaI KasI KpnI Ksp632I KspI
MaeI MamI MboII MfeI MflI
MluI MluNI MroI MscI MseI
MspI MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflMI PinAI
PmeI PshAI PshBI Psp1406I PstI
PvuI RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I TseI Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AluI 324 AGCT AG^CT
AlwNI 112 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 235 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 89 GGACC G^G[AT]CC
BbrPI 222 CACGTG CAC^GTG
BsaAI 222 CACGTG [CT]AC^GT[GA]
BsaJI 24 CCTTGG C^C[ACGT][ACGT]GG
BsmBI 233 GAGACG CGTCTC(1/5)
BssKI 15 CCTGG ^CC[ACGT]GG
BstF5I 12 CATCC GGATG(2/0)
BstNI 15 CCTGG CC^[AT]GG
DraII 88 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO109I 88 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRII 15 CCTGG ^CC[AT]GG
EcoT14I 24 CCTTGG C^C[AT][AT]GG
FokI 12 CATCC GGATG(9/13)
HphI 228 GGTGA GGTGA(8/7)
MaeIII 328 GTGAC ^GT[ACGT]AC
MslI 222 CACGTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 323 CAGCTG C[AC]G^C[GT]G
MvaI 15 CCTGG CC^[AT]GG
PflFI 235 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 330 GACTC GAGTC(4/5)
PmaCI 222 CACGTG CAC^GTG
PmlI 222 CACGTG CAC^GTG
PpuMI 88 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 323 CAGCTG CAG^CTG
ScrFI 15 CCTGG CC^[ACGT]GG
SfaNI 29 GCATC GCATC(5/9)
SmlI 162 CTTGAG C^T[CT][GA]AG
StyI 24 CCTTGG C^C[AT][AT]GG
TaqI 313 TCGA T^CGA
TfiI 131 GAATC G^A[AT]TC
Tsp45I 328 GTGAC ^GT[GC]AC
Tsp509I 174 AATT ^AATT
TspRI 42 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 235 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 313 TCGA T^CGA
XcmI 214 CCACAAGCCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 12 enzymes) #
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AlwI 211 GATCC GGATC(4/5)
282 GATCC
BsrI 337 ACTGG ACTGG(1/-1)
121 CCAGT
Cfr13I 18 GGCCC G^G[ACGT]CC
89 GGACC
DpnII 211 GATC ^GATC
282 GATC
HaeIII 18 GGCC GG^CC
193 GGCC
HinfI 131 GAATC G^A[ACGT]TC
330 GACTC
MboI 211 GATC ^GATC
282 GATC
NdeII 211 GATC ^GATC
282 GATC
NlaIII 249 CATG CATG^
279 CATG
NlaIV 157 GGCTCC GG[ACGT]^[ACGT]CC
301 GGAACC
Sau3AI 211 GATC ^GATC
282 GATC
Sau96I 18 GGCCC G^G[ACGT]CC
89 GGACC
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# 3 OR MORE CUT ENZYMES ( 3 enzymes) #
#########################################
BsmAI MaeII MnlI