Restriction map for
pFN21AE6066
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvaII AviII AvrII
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmBI BsmFI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BsrI BssHII BssSI
BstBI BstUI BstYI Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HinfI HpaI KasI KpnI Ksp632I
KspI MaeII MfeI MflI MluI
MroI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TaqI
TfiI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 895 GTATAC GT^[AC][GT]AC
AlwNI 239 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
BalI 404 TGGCCA TGG^CCA
BanI 549 GGTGCC G^G[CT][GA]CC
BciVI 455 GTATCC GTATCC(6/5)
BmyI 550 GTGCCC G[AGT]GC[ACT]^C
BpmI 279 CTCCAG CTGGAG(16/14)
BsaBI 187 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 933 GTGCAG GTGCAG(16/14)
BsmAI 241 GAGAC GTCTC(1/5)
BsmI 272 GAATGC GAATGC(1/-1)
Bsp1286I 550 GTGCCC G[AGT]GC[ACT]^C
BssKI 468 CCTGG ^CC[ACGT]GG
Bst1107I 895 GTATAC GTA^TAC
BstAPI 316 GCAGGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 468 CCTGG CC^[AT]GG
BstXI 305 CCATGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 895 GTATAC GTA^TAC
DraII 472 GGGGCCT [GA]G^G[ACGT]CC[CT]
DrdI 601 GACACCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 404 TGGCCA [CT]^GGCC[GA]
EcoO109I 472 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRII 468 CCTGG ^CC[AT]GG
EcoT14I 75 CCAAGG C^C[AT][AT]GG
HapII 729 CCGG C^CGG
HpaII 729 CCGG C^CGG
MamI 187 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 404 TGGCCA TGG^CCA
MscI 404 TGGCCA TGG^CCA
MseI 142 TTAA T^TAA
MspI 729 CCGG C^CGG
MvaI 468 CCTGG CC^[AT]GG
NspI 224 GCATGC [GA]CATG^[CT]
PstI 477 CTGCAG CTGCA^G
ScrFI 468 CCTGG CC^[ACGT]GG
SphI 224 GCATGC GCATG^C
StyI 75 CCAAGG C^C[AT][AT]GG
Tru9I 142 TTAA T^TAA
Tsp509I 183 AATT ^AATT
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# TWO CUT ENZYMES ( 17 enzymes) #
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AciI 396 CCGC CCGC(-3/-1)
573 CCGC
AfaI 210 GTAC GT^AC
655 GTAC
BfaI 86 CTAG C^TAG
542 CTAG
BsaJI 75 CCAAGG C^C[ACGT][ACGT]GG
468 CCTGGG
BsrDI 798 CATTGC GCAATG(2/0)
382 GCAATG
BstEII 527 GGTTACC G^GT[ACGT]ACC
855 GGTGACC
BstPI 527 GGTTACC G^GT[ACGT]ACC
855 GGTGACC
Cfr13I 473 GGGCC G^G[ACGT]CC
726 GGGCC
EcoO65I 527 GGTTACC G^GT[ACGT]ACC
855 GGTGACC
HphI 855 GGTGA GGTGA(8/7)
107 TCACC
MaeI 86 CTAG C^TAG
542 CTAG
MspA1I 786 CAGCTG C[AC]G^C[GT]G
844 CAGCTG
PvuII 786 CAGCTG CAG^CTG
844 CAGCTG
RsaI 210 GTAC GT^AC
655 GTAC
Sau96I 473 GGGCC G^G[ACGT]CC
726 GGGCC
SfcI 477 CTGCAG C^T[GA][CT]AG
783 CTACAG
SmlI 706 CTCAAG C^T[CT][GA]AG
808 CTCAAG
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AluI BbvI BsiYI BslI BstF5I
Cac8I DdeI DpnII Fnu4HI FokI
HaeIII ItaI MaeIII MboI MboII
MnlI MslI MwoI NdeII NlaIII
NlaIV Sau3AI SfaNI TseI Tsp45I
TspRI