Restriction map for
pFN21AE5145
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# NO CUT ENZYMES ( 185 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DdeI DraI DraII
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FauI FbaI FokI FseI
FspI HgaI Hin1I HincII HindII
HindIII HpaI HphI KasI KpnI
KspI MaeI MaeII MamI MfeI
MluI MseI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NlaIII NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TaqI TfiI Tru9I Tsp509I
TspRI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccIII 231 TCCGGA T^CCGGA
AluI 224 AGCT AG^CT
AspHI 223 GAGCTC G[AT]GC[AT]^C
BalI 54 TGGCCA TGG^CCA
BamHI 192 GGATCC G^GATCC
BglI 22 GCCCTATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 199 CTGGAG CTGGAG(16/14)
BsaI 141 GAGACC GGTCTC(1/5)
BsaWI 231 TCCGGA [AT]^CCGG[AT]
BseAI 231 TCCGGA T^CCGGA
BseRI 16 CTCCTC GAGGAG(10/8)
BsiHKAI 223 GAGCTC G[AT]GC[AT]^C
BsiYI 183 CCCCTACCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 183 CCCCTACCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 141 GAGAC GTCTC(1/5)
BsmFI 181 GTCCC GGGAC(10/14)
BspEI 231 TCCGGA T^CCGGA
BspMI 61 GCAGGT ACCTGC(4/8)
BsrI 178 CCAGT ACTGG(1/-1)
BstYI 192 GGATCC [GA]^GATC[CT]
Cac8I 52 GCTGGC GC[ACGT]^[ACGT]GC
DpnII 193 GATC ^GATC
EarI 138 GAAGAG CTCTTC(1/4)
HinfI 85 GAGTC G^A[ACGT]TC
Ksp632I 138 GAAGAG CTCTTC(1/4)
MaeIII 239 GTGAC ^GT[ACGT]AC
MboI 193 GATC ^GATC
MboII 138 GAAGA GAAGA(8/7)
MflI 192 GGATCC [GA]^GATC[CT]
MluNI 54 TGGCCA TGG^CCA
MroI 231 TCCGGA T^CCGGA
MscI 54 TGGCCA TGG^CCA
MspA1I 75 CAGCGG C[AC]G^C[GT]G
NdeII 193 GATC ^GATC
PleI 85 GAGTC GAGTC(4/5)
SacI 223 GAGCTC GAGCT^C
Sau3AI 193 GATC ^GATC
SfaNI 47 GCATC GCATC(5/9)
StuI 272 AGGCCT AGG^CCT
Tsp45I 239 GTGAC ^GT[GC]AC
XhoII 192 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AciI 38 CCGC CCGC(-3/-1)
77 GCGG
AlwI 193 GATCC GGATC(4/5)
192 GGATC
BbvI 100 GCAGC GCAGC(8/12)
125 GCAGC
BcnI 189 CCCGG CC^[GC]GG
294 CCCGG
BsgI 80 GTGCAG GTGCAG(16/14)
98 GTGCAG
CfoI 150 GCGC GCG^C
282 GCGC
Cfr13I 30 GGCCC G^G[ACGT]CC
292 GGCCC
EaeI 35 TGGCCG [CT]^GGCC[GA]
54 TGGCCA
HaeII 149 GGCGCT [GA]GCGC^[CT]
281 GGCGCT
HhaI 150 GCGC GCG^C
282 GCGC
HinP1I 150 GCGC G^CGC
282 GCGC
NciI 189 CCCGG CC^[GC]GG
294 CCCGG
Sau96I 30 GGCCC G^G[ACGT]CC
292 GGCCC
TseI 100 GCAGC G^C[AT]GC
125 GCAGC
#########################################
# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
BanII BmyI BsaJI Bsp1286I BssKI
BstNI EcoRII Fnu4HI HaeIII HapII
HpaII ItaI MnlI MspI MvaI
MwoI NlaIV ScrFI