Restriction map for
pFN21AE6267
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BbeI BbrPI BcgI BclI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaI BsiEI BsiHKAI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HhaI HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MfeI MluI MseI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I Tsp509I Tth111I TthHB8I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccIII 41 TCCGGA T^CCGGA
AcyI 278 GACGCC G[GA]^CG[CT]C
AfaI 97 GTAC GT^AC
AocI 805 CCTGAGG CC^T[ACGT]AGG
BbvCI 875 CCTCAGC CCTCAGC(-5/-2)
BbvI 50 GCAGC GCAGC(8/12)
BciVI 44 GGATAC GTATCC(6/5)
BsaHI 278 GACGCC G[GA]^CG[CT]C
BsaWI 41 TCCGGA [AT]^CCGG[AT]
BseAI 41 TCCGGA T^CCGGA
BseRI 106 CTCCTC GAGGAG(10/8)
BspEI 41 TCCGGA T^CCGGA
BstYI 828 GGATCT [GA]^GATC[CT]
Bsu36I 805 CCTGAGG CC^T[ACGT]AGG
DsaI 611 CCATGG C^C[GA][CT]GG
EarI 667 CTCTTC CTCTTC(1/4)
Eco81I 805 CCTGAGG CC^T[ACGT]AGG
EcoNI 480 CCTGGATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Fnu4HI 50 GCAGC GC^[ACGT]GC
HgaI 278 GACGC GACGC(5/10)
Hin1I 278 GACGCC G[GA]^CG[CT]C
ItaI 50 GCAGC GC^[ACGT]GC
Ksp632I 667 CTCTTC CTCTTC(1/4)
MflI 828 GGATCT [GA]^GATC[CT]
MroI 41 TCCGGA T^CCGGA
MspA1I 781 CAGCTG C[AC]G^C[GT]G
NcoI 611 CCATGG C^CATGG
PflMI 780 CCAGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 551 GAGTC GAGTC(4/5)
PvuII 781 CAGCTG CAG^CTG
RsaI 97 GTAC GT^AC
SfcI 376 CTACAG C^T[GA][CT]AG
SmaI 647 CCCGGG CCC^GGG
TseI 50 GCAGC G^C[AT]GC
Van91I 780 CCAGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 302 CCATTTACATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 828 GGATCT [GA]^GATC[CT]
XmaCI 647 CCCGGG C^CCGGG
XmaI 647 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AvaI 601 CTCGGG C^[CT]CG[GA]G
647 CCCGGG
AvaII 651 GGACC G^G[AT]CC
691 GGTCC
BalI 260 TGGCCA TGG^CCA
524 TGGCCA
BanI 616 GGTGCC G^G[CT][GA]CC
729 GGTGCC
BbsI 4 GTCTTC GAAGAC(2/6)
700 GTCTTC
BpmI 565 CTGGAG CTGGAG(16/14)
889 CTGGAG
BpuAI 4 GTCTTC GAAGAC(2/6)
700 GTCTTC
BsaAI 211 TACGTG [CT]AC^GT[GA]
676 TACGTG
BsaBI 201 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
585 GATGCTGATC
BsgI 796 CTGCAC GTGCAG(16/14)
316 GTGCAG
BsmBI 225 CGTCTC CGTCTC(1/5)
664 CGTCTC
BsoBI 601 CTCGGG C^[CT]CG[GA]G
647 CCCGGG
BstXI 170 CCATGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
206 CCATCTACGTGG
MamI 201 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
585 GATGCTGATC
MluNI 260 TGGCCA TGG^CCA
524 TGGCCA
MscI 260 TGGCCA TGG^CCA
524 TGGCCA
MwoI 331 GCTTTCTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
340 GCTTCGGGAGC
PpuMI 650 GGGACCT [GA]G^G[AT]CC[CT]
690 GGGTCCT
TfiI 243 GAATC G^A[AT]TC
630 GATTC
Tsp45I 136 GTCAC ^GT[GC]AC
406 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AluI AlwI AlwNI ApaI
BanII BcnI BmyI BsaJI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII EcoT14I FauI FokI HaeIII
HapII HinfI HpaII HphI MaeII
MaeIII MboI MboII MnlI MslI
MspI MvaI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
StyI TspRI