Restriction map for
pFN21AE6506
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# NO CUT ENZYMES ( 184 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstUI BstXI BstYI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DpnII DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MaeI MaeII MamI MboI
MfeI MflI MluI MluNI MroI
MscI MseI MslI MunI MvnI
NaeI NarI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
Sau3AI SbfI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TaqI TfiI Tru9I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AciI 271 GCGG CCGC(-3/-1)
AcsI 40 GAATTC [GA]^AATT[CT]
AfaI 93 GTAC GT^AC
AlwNI 154 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 40 GAATTC [GA]^AATT[CT]
AvaII 141 GGACC G^G[AT]CC
BcnI 303 CCGGG CC^[GC]GG
BmyI 307 GGGCAC G[AGT]GC[ACT]^C
BsaI 67 GAGACC GGTCTC(1/5)
BsiYI 298 CCTGTCCGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 298 CCTGTCCGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 115 GAGACG CGTCTC(1/5)
Bsp1286I 307 GGGCAC G[AGT]GC[ACT]^C
BsrDI 221 GCAATG GCAATG(2/0)
BstAPI 25 GCAGAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 147 GGTCACC G^GT[ACGT]ACC
BstF5I 30 GGATG GGATG(2/0)
BstPI 147 GGTCACC G^GT[ACGT]ACC
DraII 140 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 98 CCGTGG C^C[GA][CT]GG
EaeI 272 CGGCCA [CT]^GGCC[GA]
EarI 193 GAAGAG CTCTTC(1/4)
EcoO109I 140 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 147 GGTCACC G^GT[ACGT]ACC
EcoRI 40 GAATTC G^AATTC
FokI 30 GGATG GGATG(9/13)
HapII 303 CCGG C^CGG
HgaI 117 GACGC GACGC(5/10)
HinfI 226 GACTC G^A[ACGT]TC
HpaII 303 CCGG C^CGG
Ksp632I 193 GAAGAG CTCTTC(1/4)
MaeIII 148 GTCAC ^GT[ACGT]AC
MboII 193 GAAGA GAAGA(8/7)
MspI 303 CCGG C^CGG
NciI 303 CCGGG CC^[GC]GG
NlaIII 171 CATG CATG^
NlaIV 140 GGGACC GG[ACGT]^[ACGT]CC
PleI 226 GACTC GAGTC(4/5)
PpuMI 140 GGGACCT [GA]G^G[AT]CC[CT]
RsaI 93 GTAC GT^AC
ScaI 92 AGTACT AGT^ACT
SexAI 143 ACCTGGT A^CC[AT]GGT
SfaNI 31 GATGC GCATC(5/9)
StuI 295 AGGCCT AGG^CCT
Tsp45I 148 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AspI 5 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
142 GACCTGGTC
BpmI 235 CTGGAG CTGGAG(16/14)
289 CTGGAG
BsmAI 67 GAGAC GTCTC(1/5)
115 GAGAC
BsmFI 3 GGGAC GGGAC(10/14)
140 GGGAC
BsrI 234 ACTGG ACTGG(1/-1)
90 CCAGT
Cfr13I 59 GGGCC G^G[ACGT]CC
141 GGACC
DdeI 35 CTCAG C^T[ACGT]AG
131 CTGAG
HphI 87 TCACC GGTGA(8/7)
149 TCACC
MspA1I 157 CAGCTG C[AC]G^C[GT]G
216 CAGCTG
PflFI 5 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
142 GACCTGGTC
PvuII 157 CAGCTG CAG^CTG
216 CAGCTG
Sau96I 59 GGGCC G^G[ACGT]CC
141 GGACC
Tsp509I 41 AATT ^AATT
201 AATT
TspRI 7 CAGTG CA[GC]TG[ACGT][ACGT]^
45 CAGTG
Tth111I 5 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
142 GACCTGGTC
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
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AluI BbvI BsaJI BssKI BstNI
EcoRII Fnu4HI HaeIII ItaI MnlI
MvaI MwoI ScrFI TseI